Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"Respiratory Complex I"”.
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Pharmacological aspects of the inhibition of mammalian respiratory complex I
Mitochondrial complex I, a large respiratory enzyme located in the inner mitochondrial membrane, catalyses electron transfer from NADH to ubiquinone while concomitantly translocating protons across the membrane to sustain ATP synthesis. A crucial aspect of the pharmacology of complex I is …
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Studies on assembly and genetic variation in mitochondrial respiratory complex I
Complex I (NADH:ubiquinone oxidoreductase) couples electron transfer to proton translocation across the inner mitochondrial membrane, to drive the synthesis of ATP. Its distinctive L-shaped structure comprises 45 subunits, encoded by both the mitochondrial and nuclear genomes, which are assembled …
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Structural Studies into the Mechanism and Organisation of Mammalian Respiratory Complex I
Respiratory complex I (NADH:ubiquinone oxidoreductase) is the major entry point of electrons into the electron transport chain and couples its redox activity with proton pumping across the inner mitochondrial membrane, thus contributing to the proton motive force which drives ATP synthesis. With …
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Mutagenesis investigations into proton transfer pathways and control points in respiratory complex I
Respiratory complex I (NADH:ubiquinone oxidoreductase) is a large multi-subunit membrane protein that uses the energy from electron transfer from NADH to ubiquinone to transport four protons across an energy transducing membrane. The protons contribute to building the proton-motive force that …
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Cryo-EM studies of substrate and inhibitor binding to mammalian respiratory complex I
Mammalian respiratory complex I (NADH:ubiquinone oxidoreductase) is an intricate multi-subunit, energy-transducing membrane protein that is essential for aerobic energy metabolism and NADH/NAD⁺ homeostasis. It couples the energy released from NADH oxidation and ubiquinone (Q) reduction to pump four …
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Structure-function studies of respiratory complex I from Paracoccus denitrificans using membrane mimetics
Respiratory complex I (NADH:ubiquinone oxidoreductase) is a crucial metabolic enzyme that couples the free energy released from NADH oxidation and ubiquinone reduction to translocate four protons across energy-transducing membranes, contributing to the proton motive force that powers oxidative …
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Paracoccus denitrificans as a model system for studying the mechanism of respiratory complex I
Respiratory complex I (NADH:ubiquinone oxidoreductase) is a crucial metabolic enzyme that couples the free energy released from NADH oxidation and ubiquinone reduction to the translocation of four protons across an energy-transducing membrane, contributing to the proton motive force used to …
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Electron cryomicroscopy structures of respiratory supercomplexes from alphaproteobacteria suggest mechanisms to enhance catalysis and to prevent deactivation of respiratory complex I
In mitochondria, the electron transport chain complexes that generate the proton motive force to drive ATP synthesis form oligomeric membrane assemblies known as supercomplexes. Even though they are found throughout nature and several physiological consequences are associated with their depletion, …
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Computer-Aided Drug Discovery for Helicobacter pylori
… novel Hp thienopyrmidine inhibitors that target respiratory complex I, an essential enzyme in respiration. Respiratory complex I is a large asymmetric multidomain and membrane bound enzyme and due to these innate features, it is not practical for biophysical or biochemical enzyme screening making …
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Structural and functional studies of mitochondrial NADH:ubiquinone oxidoreductase (complex I)
NADH:ubiquinone oxidoreductase (complex I) is the largest and most complicated enzyme in the mitochondrial electron transfer chain. It catalyses the oxidation of NADH and the reduction of ubiquinone, coupled to the translocation of protons across the mitochondrial inner membrane, maintaining the …
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The Discovery and Development of Thienopyrimidines as Inhibitors of Helicobacter pylori
… These experiments identified H. pylori’s respiratory Complex I as the putative target of the series, with the amino acid changes found in the NuoD subunit. Lead compounds demonstrated efficacy in an ex vivo model but not in the in vivo studies in a H. pylori murine infection model, …
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A Multi-Factorial Assessment of Partial Mitochondrial Complex I Impairment in Colorectal Cancer
… metabolism, particularly the function of respiratory complex I, has emerged as a key determinant of tumor bioenergetics and signaling. This dissertation investigates how partial complex I dysfunction influences CRC tumor growth and whether the impact of this partial disruption depends on …
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Cryo-electron microscopy studies on ovine mitochondrial complex I
… to determine the atomic structure of mammalian respiratory complex I. Mitochondrial complex I (also known as NADH:ubiquinone oxidoreductase) is one of the central enzymes in the oxidative phosphorylation pathway. It couples electron transfer between NADH and ubiquinone to proton translocation …
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Mechanism of Metformin Action in Dermal Fibroblasts
… is dysregulated AMPK signaling and mitochondrial complex activity, which regulates both glucose uptake and cellular energy status. Current therapeutic methods to counter hyperglycemia include tight euglycemic control and/or intensive insulin treatment, which are associated with increases in …
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Metabolic downregulation during diapause in embryos of Artemia franciscana
… of <i>Artemia franciscana</i> undergo a dramatic respiratory depression upon release from the adult female as they enter a state of hypometabolism termed diapause. The mechanisms by which such a respiratory depression is achieved remain unexplained. Evidence presented here shows that strategic …
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Investigating [NiFe]-hydrogenases in γ-Proteobacteria
… the [NiFe]-hydrogenases, which all harbour a complex NiFe(CN-)2CO active site in the ‘large’ catalytic subunit and usually have three iron-sulfur clusters within a ‘small’ electron transferring partner subunit. [NiFe]-hydrogenases have undergone massive diversification, with four major …
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Investigating the rate-limiting step of mitochondrial complex I catalysis
Respiratory complex I (NADH:ubiquinone oxidoreductase) is a key enzyme in metabolism and is the least understood protein in the mitochondrial electron transfer chain (ETC). It couples the energy released from NADH oxidation and ubiquinone (Q) reduction to the translocation of four protons across …
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Investigating the anti-leukaemic effect of tamoxifen in the myeloproliferative neoplasms
… (4OH-TAM) directly inhibited mitochondrial respiratory complex I and decreased adenosine triphosphate (ATP) synthesis. This reduction of intracellular ATP significantly suppressed the cytokine-induced pathogenic activation of JAK2 and STAT5 in JAK2<sup>V617F+</sup> human cell lines, which …