Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 22 for “"Reporter mice"”.
-
Characterization of Fasting-Induced p21 Expression and Protection of Intestinal Stem Cells
… forms of stress, we generated knock-in imaging reporter mice that express firefly luciferase (<italic>FLuc</italic>) under the control of the endogenous p21 promoter. I demonstrated that FLuc expression and bioluminescence detection mirrored endogenous p21 protein levels and promoter activity in …
-
Sources of Pulmonary GM-CSF and Its Roles in Myeloid Cell Homeostasis
… Utilizing newly developed transgenic Csf2-reporter mice, we aimed to profile GM-CSF expression in various barrier tissues, including the lung and skin. In the lung, we detected Csf2-reporter expression in immune cells such as group 2 innate lymphoid cells and γδ T cells, as well as in …
-
Characterization of Tyrosine Hydroxylase-Positive Neurons Projections from the Paraventricular Nucleus of Hypothalamus to Hindbrain Autonomic Regulatory Centers
… our goal, Cre recombinase-dependent TIT2L-eGFP reporter mice were microinjected with adeno-associated virus encoding the Cre recombinase driven by rat TH promoter (AAV2-rTHP-Cre, 6.8 x 1011 gc) into the PVN using a stereotaxic apparatus. One month after injection, mice were transcardially …
-
Wnt Secretion Proteins Modulate RANKL-Induced Expression of Aire in Thymic Epithelial Cells
… various TEC populations in TCF/Lef:H2B/GFP Wnt reporter mice. Here we show that previously uncharacterized populations of postnatal mTECs undergoing canonical Wnt signaling differentially express Wnt proteins and we further identify Wnt-5a and Wnt-5b as possible candidates for regulating Wnt …
-
Paneth Cell-Dependent Intestinal Homeostasis During T. Gondii Infection
… a number of fluorescent Paneth cell-specific reporter mice to demonstrate that acute T. gondii infection leads to Paneth cell death by an IFN-g-dependent pathway. Our results also revealed that constitutive elimination of Paneth cells does not enhance susceptibility to T. gondii infection, …
-
Identification of novel regulators of lymphocyte development and differentiation using CRISPR-Cas9 screens
… in TH2 cell differentiation. The generation of mice with a conditional deletion of αv in T cells corroborated this essential role in TH2 cell differentiation. To screen for novel regulators of ILC2 development, a new ILC culture system was optimised in which purified common lymphoid progenitors …
-
Targeting transcriptional and translational mechanisms to enhance utrophin A expression as a therapy for Duchenne muscular dystrophy
… program. Treatment of dystrophin-deficient mdx mice with GW501516 stimulated utrophin A expression at the sarcolemma and mitigated the dystrophic phenotype, as evidenced by increased sarcolemmal stability and decreased susceptibility of skeletal muscles to lengthening contractions. Under …
-
Role of the ARF Tumor Suppressor in Osteoclasts
… Surprisingly, we generated Arf -/- mice as demonstrated by genotyping, loss of ARF expression in the testis, and a phenotype comparable to traditional Arf -/- mice. Furthermore, both Cathepsin K and Cre are expressed in reproductive tissues, which results in Cre activity within …
-
Rgs16 is a Pancreatic Reporter of Chronic Hyperglycemia in Diabetes
… Rgs16::GFP expression. We investigated diabetic mice to determine if Rgs16::GFP would reactivate during beta cell expansion in adulthood. The type 1 diabetic models of beta cell death were streptozotocin (STZ) treatment and PANIC-ATTAC mice. Type 2 diabetic models consisted of ob/ob mice and diet …
-
Delineating the molecular mechanisms regulating chondrogenesis
… within the Sox9 promoter. Luciferase-based reporter assays in mouse revealed a proximal promoter spanning – 2 kb from the transcriptional start site, while mobility shift assays demonstrated that a CCAAT motif is involved in the transactivation of Sox9. Moreover, luciferase-based reporter …
-
Effects of Binge Alcohol Exposure on Canonical Wnt Signaling During Fracture Repair
… test this, C57BL/6 or beta-catenin/TCF-reporter male mice were exposed to intraperitoneal alcohol or saline for 3 consecutive days for either 1 week or 2 week cycles. One hour after the final injection, mice received a stabilized tibial fracture and were sacrificed 1,2,3, 6, 9, or 14 …
-
Immunoregulation of the central response to peripheral nerve injury: motoneuron survival and relevance to ALS
Facial nerve axotomy (FNA) in immunodeficient mice causes significantly more facial motoneuron (FMN) loss relative to wild type (WT), indicating that the immune system is neuroprotective. Further studies reveal that both CD4+ T cells and interleukin 10 (IL-10) act centrally to promote neuronal …
-
Investigating the role of macrophage-epithelial cell interaction in salivary gland regeneration
… occur via the CX3CL1-CX3CR1 axis. Using CX3CL1 reporter mice I confirm that KRT14+ cells produce CX3CL1, the sole ligand for CX3CR1, providing robust evidence that macrophages directly communicate with KRT14+ cells during the repair process. Indeed, upon the depletion of macrophages …
-
Tetracycline-dependent gene expression in cholinergic neurons of transgenic mice : a tool to study Neuregulin-1 function in vivo
… by oocyte injection. The ChAT-tTA2S transgenic mice were subsequently characterized by in situ hybridization and by mating them to reporter mice carrying a tTA-inducible ?-galactosidase gene. In two independent transgenic founder lines, a faithful cholinergic expression was observed. In …
-
Mechanisms for Osteoblast and Osteocyte Initiation and Sustainment of Bone Formation in Young-Adult and Aged Mice
… any processes that are disrupted in aged mice can aide in the development of new anabolic drugs for treating diseases like osteoporosis. This thesis begins by investigating the genes expressed by osteocytes following loading at an early mechanosensitive (4-hr) timepoint, and later at a …
-
Fibroblasts: Key Cells in Inflammation and Fibrosis
… II (Ang II) infusion into normolipidimic mice leads to the development of aortic dissections and abdominal aortic aneurysm (AAA). IL-6 is a pro-inflammatory cytokine that is secreted by fibroblasts and macrophages in the aortic adventitia and promotes the development of aortic dissections. …
-
Deconstructing G Protein-Coupled Receptor Dimer Pharmacology: Case Studies in Dopamine D1 and D2 Receptors
… using bacterial artificial chromosome-D1 and D2 reporter mice that D1 and D2 receptors are co-expressed in a small number of brain regions, we failed to observe D1-D2 receptor colocalization, suggesting the possibility that in neurons the receptors are somehow segregated.
-
The impact of diet-induced obesity and exercise on adipose-resident stem cells
… were conducted. In the first, male C57BL/6J mice were placed on control or high fat diet (HFD) for 8 wk prior to and following initiation of a 16 wk treadmill exercise program. ADSCs (Sca-1+CD45-) were isolated from epididymal adipose tissue and mRNA was evaluated using high throughput qPCR. …
-
The role of the bone marrow niche and G-CSF in osteochondral repair
… in the typically poor healing C57Bl/6 strain of mice. Further, cells known to respond to G-CSF were seen to have specific spatial profiles in the osteochondral repair tissue over time. For example at 24 hours post-surgery, defect sites were filled with a blood clot within which neutrophils (anti …
-
PTEN regulates the BMI1-mediated self-renewal of long-term hematopoietic stem cells in developmental and stress conditions
… in the LT-HSCs of VE-cadherin-cre+;Ptenfl/fl mice does not lead to the loss of HSC self-renewal, raising the possibility that PTEN-mediated self-renewal regulation may be more complicated than previously reported. To understand the molecular regulation of HSC homeostasis, I crossed Bmi1GFP/+ …
Page 1 of 2