Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 6 of 6 for “"Reporter cell lines"”.
-
Role of cancer stem cells in breast and prostate cancer
Cancer stem cells (CSC) are thought to be responsible for the initiation, propagation and tumour re-occurrence. CSC have been identified in most solid and haematological cancers and account for ~1% of the total cell population. Culturing cancer cell lines in monolayers enriches for the most …
-
Investigation of the SCN 2.2 Cell Line as a Model of the Central Mammalian Circadian Pacemaker
The SCN 2.2 cell line, derived by immortalization of fetal rat suprachiasmatic nucleus (SCN) tissue, was investigated for characteristics of the SCN, which is the central mammalian circadian pacemaker. Extensive characterization of differentiated SCN 2.2 cultures revealed that this cell line …
-
Development and validation of a cell based model of insulin resistance and investigation into the intracellular molecular defects induced by diabetes and obesity
… this thesis establishes that culturing liver cells in sera from obese diabetic patients reduces the ability of insulin to repress the key gluconeogenic gene, phosphoenolpyruvatecarboxykinase (PEPCK). Cells cultured in serum from obese diabetic human subjects exhibited defective PEPCK mRNA …
-
PROTEINi screens identify ORP9 as a novel regulator of KRAS-driven signalling
… alongside additional pathways that promote cellular proliferation, survival and migration. KRAS traffics to the plasma membrane in order to be activated and bind to its effector proteins. Despite almost 40 years of research, there has been limited success in direct targeting of KRAS, as well …
-
Understanding the intracellular signalling pathways activated by lipid nanoparticle delivery of mRNA
… to transfect exogenously transcribed mRNA into cells for prophylactic or therapeutic use. LNP delivered mRNA (LNP/mRNA) in the clinic can induce a transient, innate immune response resulting in local and systemic inflammation, which is a tolerability issue for therapeutic applications. The …
-
Investigating Wnt3-mediated symmetry breaking and cell fate in engineered gastruloids
… 2005). In contrast, aggregates of embryonic stem cells (ESCs) termed gastruloids are able to display symmetry breaking and develop an AP axis in the absence of extraembryonic tissues or localised exogenous positional signalling (Baillie-Johnson, van den Brink, Balayo, Turner, & Arias, 2015; …