Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 250 for “"Reporter Gene"”.
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Photoacoustic Reporter Gene Imaging And Optical Coherence Computed Tomography
… we established a new paradigm to visualize gene expression in vivo based on optical absorption. In the post-genomic era, we are now being challenged to develop novel molecular imaging methods to identify the functions of genes. PAI can detect specific molecules according to their …
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Rapid, Quantitative Assessment of Antimycobacterial Water Disinfection based on the Firefly Luciferase Reporter Gene
… of mycobacteria using firefly luciferase as a reporter gene for disinfection survival studies. Derivatives of M. avium strains MD1 and A5, Mycobacterium smegmatis strain VT307 and Mycobacterium bovis BCG strain Pasteur carrying the firefly luciferase gene (pLUC10) were constructed. In …
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Use of bgaH as a reporter gene for studying translation initiation in the archaeon Haloferax volcanii
The bgaH gene isolated from Haloferax lucentensis codes for P-galactosidase. To study the function of initiator tRNAs in translation initiation in Haloferax volcanii, the initiator AUG codon of the bgaH gene was mutated to UAG, UAA, UGA, and GUC. Four different H. volcanii initiator tRNA derived …
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DEVELOPMENT OF AN IN VIVO MULTITASKING BIOLUMINESCENT REPORTER GENE PLATFORM FOR THE PRECLINICAL EVALUATION OF RNA THERAPEUTICS
… of medicines, enabling precise modulation of gene expression through sequence-defined mechanisms and offering novel therapeutic opportunities for previously intractable diseases. The potential of these drugs to address unmet pharmacological needs, together with their novelty and …
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The effect of different sample preparatory protocols on the induction of the aryl hydrocarbon receptor (AhR) in the H4IIE–luc reporter gene bio–assay
… of dioxins using the H4IIE-luc-reporter gene assay. The protocols evaluated were either Soxhlet or pressurised liquid extraction (PLE) combined with a manual acid digestion, gel permeation chromatography (GPC) and Florisil fractionation clean-up procedure as well as the automated …
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Transcriptional Regulation of Neurogenic Atrophy-Induced Gene Expression by Muscle Ring Finger-1 and Myogenic Regulatory Factors
… a transcriptional modulator of atrophy-induced gene activity, including the regulation of MAFbx and MuRF1 expression. To characterize the transcriptional regulation of MuRF1 and MAFbx, reporter gene constructs containing fragments of the proximal promoter regions of these genes were developed, …
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Cγ- and Cα-Subunits Provide the Specificity for cAMP/PKA Function
… and bacteria, respectively, were purified to homogeneity. Kinetic analysis showed that Cγ and Cα shared primary substrate phosphorylation specificity. Cγ exhibited similar or lower Km values, but lower Vmax values in substrate phosphorylation than Cα. Different isoforms of PKI and R-subunit …
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Role of an Adenylyl Cyclase Isoform in Alcohol's Effect on Cyclic AMP Regulated Gene Expression in Mammalian Cells
… CREB-CRE regulated expression of various genes is implicated in the predisposition to and development of alcoholism in humans. Alcohol also induces changes in inflammatory and immune responses; these changes increase the incidence of pneumonias and other infections, which can negatively …
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Expression of Gus Gene in Tobacco (Nicotiana tabacum) and Corn (Zea mays) Using Dimboa-Resistant Agrobacterium Strains
… microbial infection. Agrobacteriummediated genetic transformation technique can be employed to genetically alter most dicotyledonous plant species. However, such transformation has been limited in monocotyledonous plants, especially corn. One of the factors responsible for this recalcitrance …
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In Vitro Regeneration and Genetic Transformation of Sesbania Drummondii: A Medicinally and Environmentally Important Plant
… found suitable for root induction. In vitro regenerated plants were acclimatized in greenhouse conditions. This report is a first report on studies of in vitro regeneration of S. drummondii. grobacterium tumefaciens, bearing the plasmid pCAMBIA 1305.1, was used to develop a genetic …
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Controlling Gene Expression with Synthetic Molecules
Aberrant gene expression patterns have been implicated in several pathological states. Synthetic molecules capable of functionally mimicking native transcription factors and regulating gene expression in a specific and predictable manner may represent a new paradigm in drug development. Native …
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I. Identification of thecis-regulatory elements of the Arabidopsis thaliana TCH3 gene. II. Developmental and molecular responses to touch stimulation in Arabidopsis mutants defective in ethylene responses
… by upregulation of expression of the TCH genes. The TCH3 transcription unit is 1522 base pairs (bp) and contains three 367-bp repeats which are highly identical. TCH3 encodes a 324 amino acid calcium-binding protein which is 58-60% identical to the Arabidopsis calmodulins and contains six …
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The Association of the Human Cytomegalovirus Genome with the Cellular Nuclear Matrix
… regulatory region of the major immediate early gene (MIE) of the human cytomegalovirus (HCMV) has been analyzed for its capability of fulfilling criteria defining nuclear scaffold/matrix attachment regions (S/MARs). These criteria are: 1) binding the nuclear matrix in an in vitro nuclear matrix …
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TAXI : a new vehicle for the transfer of genes into monocotyledonous plants
The transfer of foreign genes into cereals followed by their correct expression in a tissue specific, developmentally regulated manner has become an important research focus. Based on the mechanism of Agrobacterium tumefaciens mediated transformation in dicots, a modified method to transform the …
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Cloning and characterization of glycogen synthase from Dictyostelium discoideum
… In order to clone the 3’ portion of the gene it was necessary to develop a new technique, enrichment-PCR, that relies on the base composition of the Dictyostelium genome. Due to the high A+T content of the Dictyostelium genome, a polyT primer and a gene specific primer were used to …
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Human Estrogen Receptor(alpha) Activity on Estrogen Responsive, Integrated Chloramphenicol Acetyltransferase (Cat) Reporter Constructs in Chinese Hamster Ovary (Cho) Cells
… the first report of activation of an integrated reporter gene by this activation function.
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Promoter analysis in transgenic sugar beet
Crop improvement by gene manipulation will often require subtle regulation of expression of foreign genes in the transgenic plants. A strategy has been defined for production of high sucrose yield ! high juice purity / low environmental impact varieties of sugar beet. This requires modification of …
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Development of a novel imaging system for cell therapy in the brain.
… the human sodium-iodide symporter (NIS) as a reporter gene system for in vivo imaging of neural stem cells in the brain. My data show that the NIS, in combination with SPECT/CT imaging, allows for the repeated visualization of neural stem cells in vivo, and validate the use of the NIS as a …
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Characterisation of an Arabidopsis mutant with altered greening characteristics
… show expression of nuclear photosynthesis genes in the presence of norflurazon, which results in chloroplast photooxidation, or in the presence of lincomycin, an inhibitor of plastid translation. The aim of this study was to understand the role of plastid protein synthesis in …
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