Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"Replicons"”.
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Conjugation of extrachromosomal replicons of Rhodococcus erythropolis AN12
Bacteria belonging to the Gram-positive actinomycete species, Rhodococcus erythropolis, are diverse not only in terms of metabolic potentials but the plasmids they encode. Pulsed-field gel electrophoresis (PFGE) revealed three previously uncharacterized megaplasmids in the genome of Rhodococcus …
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Identification and characterization of cell-adapted mutations in West Nile Virus and replicons
Flavivirus persistence in cell culture is achieved by altering the interaction between the viral genome and the host cell. To identify some of the factors that contribute to a persistent West Nile virus (WNV) infection, WNV subgenomic replicon (WNR) genomes were modified to contain neomycin …
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Molecular and genetic analysis of RepA from the P307 RepFIB replicon
… placed RepFIB into the Step function class of replicons. The placement of RepFIB within this group, as well as a strong homology between RepFIB and mini-P1, has resulted in a series of predictions concerning the control elements of RepFIB replication. The aim of this work was to test some of …
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Viral and Host Genetic Determinants of Hepatitis C Virus Persistence and Interferon Resistance
… We also demonstrated that HCV subgenomic RNA replicons can be used to model the early events of HCV infection. We found that HCV RNA replicons rapidly induce the cellular antiviral response upon their transfection into host Huh7 cells and we determined that intracellular HCV double stranded …
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Genomic diversity of Lactobacillus salivarius
… by sequencing to harbour four extrachromosomal replicons, a circular megaplasmid (pMP1046A), a putative chromid (pMP1046B), a linear megaplasmid (pLMP1046) and a smaller circular plasmid (pCTN1046) which contains an integrated Tn916-like element (Tn6224), which carries the tetracycline …
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Investigating the genome complexity of Streptomyces clavuligerus
… Additionally, the telomeres of the five replicons were purified by a self ligation method, and identification of the sequences confirmed that the five replicons carry archetypal telomeres, with the chromosome and megaplasmid sharing identical telomeric sequences. Streptomycetes telomeres …
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Inhibition of Cell Proliferation through Regulated Intramembrane Proteolysis of CREB3L1
… be silenced in proliferating cells that harbor replicons of HCV or WNV. Our results indicate that CREB3L1 may play an important role in limiting virus spread by inhibiting proliferation of virus-infected cells. Doxorubicin is used extensively for chemotherapy of diverse types of cancer, yet the …
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Post-Replicative Resolution of Under-Replication
… binds to chromatin associated with large replicons, and functions synergistically with dormant origins to protect the stability of the genome. Additional candidates, inspired by common fragile site research, have also been characterised as responders to spontaneous under-replication, and …
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The Multifunctional Capsid Gene of Hepatitis C Virus
… dispensable for the viability of subgenomic replicons, strong selective pressure for the integrity of these structures, both in vivo and in vitro, highlights their importance for the HCV lifecycle.</p>
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Deoxyribonucleic acid restriction/modification systems and gene transfer strategies in Ruminococcus albus 8 and Ruminococcus flavefaciens FD-1
… the consequences of incompatible plasmid replicons and failure in the expression of selectable markers cannot be overlooked. Fluorescent labelled dextrans were used in place of plasmid DNA and indicated that electroporation resulted in the uptake of macromolecules. Type-II endonuclease …
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Comparative microbial genome analysis of lactobacilli
… and proteases vary considerably on all replicons while two exopolysaccharide clusters show a lot of variation on the chromosome. In Chapter 4, I focussed on the evolutionary rates of lactobacilli, divided into 10 phylogenetic sub-clades based on an ongoing study (Salvetti et al; in …
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Investigations into the use of maize streak virus as a gene vector
… a gene vector. These involved testing MSV-based replicons in transgenic cell lines, in transient expression assays in maize cells and in an infectious gene expression system in plants. MSV vectors which contained three different versions of a bar (bialaphos resistance) expression cassette in …
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Modulation of the immune response by current and novel treatments for hepatitis C virus
… expression, inhibition of replication of HCV replicons in Huh 7 cells, enhanced secretion of anti-viral and pro-inflammatory cytokines, up-regulation of NK cell activation. These findings suggest that SM-360320 might provide complementary and additional mechanisms of action to current HCV …
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Immunogenic assessment of plant-produced Human papillomavirus type 16 chimaeric L1:L2 virus-like particles and the production of an encapsidated therapeutic DNA vaccine candidate
… of infecting mammalian cells and encapsidated replicons expressed E7SH showing the promise of this candidate vaccine as a future combination prophylactic and therapeutic vaccine.
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Identification of host cellular factors that regulate human norovirus replication
The human norovirus (HuNoV) is one of the most predominant causes of gastroenteritis, yet no suitable therapeutics are available for its control. Currently, our knowledge of the precise cellular processes that control its replication is limited. In this study, we used the virus replicon system in …
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Improvements in codon usage analysis for a more detailed understanding of genome content and horizontal gene transfer
… bacterial genomes that contain multiple replicons. In A. tumefaciens we discover that the two plasmids are closely related, despite being independently conjugative. By using the mode algorithm on the B. burgdorferi genome, we are able to demonstrate a higher resolution of codon usage …
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The interaction of hepatitis C virus and intracellular lipid metabolism
… controlled expression of the NS proteins. RNA replicons were transcribed and electroporated into HuH-7 cell lines. A transiently expressing replicon was made by replacing the neomycin gene with a firefly luciferase gene. Cells expressing neither the constitutively nor the transiently genotype 3 …
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Epidemiology of antimicrobial resistance at the livestock-human interface in an urban environment: a One Health approach
… found a diversity of known plasmids and plasmid replicons that were associated with the distribution of acquired AMR genes. To conclude, I discuss the findings of this thesis in the context of the current epidemiology of AMR pathogens at the human-livestock interface and highlight future …
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