Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 45 for “"Replication initiation"”.
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Developmental regulation of DNA replication initiation in Drosophila
… a powerful system for the study of metazoan DNA replication. Amplification produces 100kb gradients of amplified DNA through repeated rounds of origin firing and bidirectional movement of replication forks from these origins. The Drosophila Follicle Cell Amplicon at the cytological location 62D, …
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The Roles of the Mcm2-7 Tails in Replication Initiation
DNA replication is complex biological reaction that spans two stages of the eukaryotic cell cycle. In G1 phase, preparation for replication begins when two copies of the replicative helicase, the Mcm2-7 complex, are loaded onto DNA. However, these helicases are loaded in an inactive state, and are …
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Mechanism and importance of Mcm2-7 double-hexamer formation during DNA replication initiation
… and accurately in each cell cycle. Thus, DNA replication is a highly-regulated multi-step process that ensures the genome is duplicated only once per cell cycle. In eukaryotic cells, initiation of DNA replication begins with loading of two heterohexameric Mcm2-7 helicases around origin DNA …
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Analysis of metazoan DNA replication initiation using Drosophila gene amplification as a model system
… and developmental regulation of DNA replication in vivo. We mapped all follicle cell amplicons using a comparative genomic hybridization strategy and identified two new amplicons. We determined the precise localization of the origin recognition complex (ORC) on a genome-wide level and …
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Multiple roles of the replication initiation protein Cdtl during helicase loading in S. cerevisiae
… propagation. In eukaryotic cells, chromosomal replication is carefully coordinated with the cell cycle to ensure that the entire genome is replicated exactly once prior to cell division. Underpinning this coordination is the tightly regulated loading and activation of the eukaryotic replicative …
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The effect of mutant replication initiation proteins and antisense RNA to the gene on maize streak virus replication
Maize (Zea mays L) is a major crop plant belonging to the family Poaceae, which includes other important cereals such as wheat, rice, barley and oats (Kellogg, 1998). Other grass crops are rye, common millet, finger millet, teff, sugarcane and sorghum. Many of these are important food crops in most …
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Delineation of the molecular mechanisms underlying DNA replication initiation and changes in gene copy number during Drosophila development
The study of differential DNA replication programs in Drosophila has provided important insight into the molecular control of replication initiation and fork progression during development. We investigated the mechanisms by which binding of the origin recognition complex (ORC) and replication fork …
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Study of mechanism of genetic death in Escherichia coli in response to A) dTTP starvation and B) combination of temperature sensitive defects in replication initiation and double strand break repair
At the cellular level life can be described as replication of metabolism. Cells first grow by continuous synthesis of RNA, protein and lipids. Once they reach a certain dimension, they duplicate their genomes, which is followed by the division of the mother cell into two daughter cells, capable of …
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Investigation of Cdc45 function during helicase activation
Eukaryotic DNA replication requires the stepwise loading of Mcm2-7 helicase complexes at origins of replication. These loaded helicases need to be activated during DNA replication initiation to unwind the DNA double helix and provide a ssDNA template for replicative DNA polymerases. Eukaryotic …
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Regulation of DNA replication and the replication initiator, DnaA, in Bacillus subtilis
DNA replication is a highly regulated process across all organisms. Improper regulation of DNA replication can be detrimental. I identified an overinitiating, conditional synthetic lethal mutant of Bacillus subtilis. I isolated suppressors of this mutant and uncovered novel genes associated with …
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Regulation of DnaA as a transcription factor by modulation of cooperative binding, and by arrA, an antisense RNA
DnaA is both the bacterial replication initiator and a transcription factor. Both activities are highly conserved and closely regulated. The mechanisms regulating its replication initiation activity have been well studied. Most organisms regulate both the abundance DnaA protein and its activity …
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Origins of non-coding Y RNA family and its role in DNA replication
… RNAs that are required for chromosomal DNA replication initiation in vertebrates. Specifically, Y RNAs play an essential role in the establishment of new DNA replication forks during G1 to S phase transition. However, the mechanism by which Y RNAs contribute to initiation of DNA replication, …
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Regulation of DNA replication by PP2A family phosphatases
… and only once per cell cycle from hundreds of replication initiation sites (origins) across the genome. This process is strictly controlled as mis-regulation can cause replication stress and genome instability, characteristics of cancer. S phase Cyclin-dependent kinase (S-CDK) plays a dual role …
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Identifying a role for Ciz1 in the DNA damage response
… protein previously shown to stimulate DNA replication initiation and facilitate cyclin exchange at pre-replication complexes. Ciz1 contains several SQ/TQ motif clusters, suggesting it is a PIKK substrate. This thesis investigates the potential role of Ciz1 in the DDR. A cell free system …
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Molecular Characterization of Mth203 protein
… available concerning eukaryotic and bacterial replication, less is known about the molecular basis of replication initiation in archaea. In general, the archaeal proteins are simplified versions of their eukaryotic counterparts. Therefore, they are potentially simple model systems to understand …
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Molecular characterization of Mth203 protein
… available concerning eukaryotic and bacterial replication, less is known about the molecular basis of replication initiation in archaea. In general, the archaeal proteins are simplified versions of their eukaryotic counterparts. Therefore, they are potentially simple model systems to understand …
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The genome-wide analysis of the specification and activation of human chromosomal DNA replication origin sites
Background: Human DNA replication is a critical cellular process that initiates at tens of thousands of DNA replication origins. Next-generation sequencing (NGS) enabled the elucidation of some characteristics that define human replication origins. Initiation-site sequencing (iniSeq) utilises the …
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Termofilinių bakterijų genetinė transformacija /
… on Dam and Dcm methylation as well as different replication initiation proteins. After the transfer of methylated and non-methylated plasmids containing various replication initiation proteins, no colonies were obtained indicating these factors do not have an impact on transformation efficiency …
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Genetic and biochemical interactions between the DNA replication initiator and a chromosome architecture protein in Bacillus subtilis
… and epistatic relationship between the DNA replication initiator DnaA and the nucleoid-associated protein Rok in Bacillus subtilis. Using ChIP-seq, I characterized the genome-wide association of DnaA and its regulator YabA. I found that DnaA and YabA associated with over 30 chromosomal …
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Genetic and molecular characterization of origins of replication from beta-lactamase-producing plasmids of Neisseria gonorrhoeae.
… characterization of the multiple origins of replication of the gonococcal beta-lactamase-producing plasmids. The Asia-plasmid, pJD4, a prototype gonococcal beta-lactamase-producing plasmid, is 7,426 bp and contains two large, direct repeats (DR-30A, 507 bp and DR-30B, 509 bp) which are …
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