Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 3 of 3 for “"Rep helicase"”.
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Single molecule force spectroscopy of single stranded DNA binding protein and Rep helicase
… time study on the conformational control of Rep helicase using an optical trap. We found that crosslinking-mediated conformational arrest of a dynamic subdomain in so-called “closed” orientation converted the Superfamily I (SFI) helicase, Rep, from a very poor DNA helicase into a powerful …
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Uncovering vital molecular contacts within the replisome and characterizing fail-safe mechanisms ensuring genomic stability in E. coli.
To study replisome coupling interactions and gain a better understanding of enzymatic communication, we modeled interfacial residues between α-Pol III and τ-CLC. A critical C-terminal linker in the τ-subunit tethers α-Pol III to the DnaB helicase for efficient DNA replication in E. coli. Therefore, …
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Exploring Novel Techniques for the Single Molecule Toolkit: Vesicle Encapsulation and Immobilization
… on a glass surface. However, there have been reports of variability of surface environment, and suspicion that observed heterogeneity of dynamic properties in single molecules might be an artifact of the local surface. A striking example is the hairpin ribozyme which was shown---in our …