Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"Regulatory RNA"”.
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Discovery and Validation of New Regulatory RNA Elements in Chlamydia trachomatis
… Relatively little is known about the regulatory mechanisms that control temporal gene expression during the developmental cycle or the control of persistence and reactivation. Here we have examined the expression of a newly defined family of non-coding RNAs (ncRNAs) that are …
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Characterization of an Orphan Riboswitch: Identification of a Metal-Sensing Regulatory RNA
Riboswitches are RNA-based genetic control elements found in untranslated regions of the mRNA transcript that they regulate. These RNA motifs are highly structured and bind metabolites to elicit control of gene expression. Typically, the metabolite sensed by these RNAs is a component of the …
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Characterizing a Small Regulatory RNA in Brucella abortus Linked to Outer Membrane Stress Resistance
… macrophages and dendritic cells. Numerous small regulatory RNAs (sRNAs) were found to be expressed by B. abortus, and it was hypothesized that they may be important for virulence. One sRNA, when deleted, was shown to be linked to outer membrane stress resistance and was named MssR (membrane …
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Investigating Hfq-dependent Small Regulatory RNA, Bsr34, and Its Roles in Brucella abortus Physiology and Pathogenicity
… regulation for survival within host cells. Small regulatory RNAs (sRNAs), often chaperoned by Hfq, are key post-transcriptional regulators of bacterial virulence and stress adaptation, yet remain largely uncharacterized in Brucella. Following the identification of Hfq-associated sRNA candidates in …
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Tools for engineering multicellular systems through cell sorting and cell state detection
… endogenous gene itself. We show that placing a regulatory RNA downstream of a terminator allows for expression of the regulatory RNA, and demonstrate this method for miRNAs and gRNAs. Together, this thesis develops methods to create synthetic cell states that can be used to control …
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EXPANDING THE TOOLKIT: STRUCTURE, DYNAMICS, AND DRUG INTERACTIONS OF THE “PRIMING LOOP” FROM HEPATITIS B VIRUS PRE-GENOMIC RNA BY SOLUTION NMR SPECTROSCOPY
RNAs are dynamic macromolecules that function as essential components of biological pathways that result in human disease, making them attractive therapeutic targets. Yet, RNA structural biology lags significantly behind that of proteins, limiting mechanistic understanding of RNA chemical biology. …
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Regulatory Genes of the Bacteroides Conjugative Transposon, CTnDOT
… of rteC. In order to determine whether CTnDOT regulatory genes can affect the expression of chromosomal genes in B. thetaiotaomicron , I used GeneChip and quantitative RT-PCR analyses. I showed that RteA and RteB are global regulators that induce the expression of various genes, in particular, …
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RNA-based portable genetic circuits and synthetic plasmids
… logic gates. Recently, the development of novel RNA regulators poses an opportunity to overcome this challenge. In this thesis work, we aim to use RNA-based strategies to improve the modularity of biological systems on three different levels: chassis modularity, circuit modularity, and part …
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Identification of RBPMS as a smooth muscle master splicing regulator via association of its gene with super-enhancers
Alternative splicing (AS) is primarily regulated by regulatory RNA-binding proteins (RBPs). It has been suggested that a small number of master splicing regulators might control cell-specific programs and these regulators could be identified via the association of their genes with transcriptional …
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Robustness and tunability in biological networks/ by Shankar Mukherji.
… counterparts. To explore how a simple regulatory element can be both robust and tunable, we turn our attention to gene regulation by microRNA (miRNA). MiRNAs are short regulatory RNA molecules that repress gene expression in a sequence-dependent manner. By observing the strength of …
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Regulatory RNAs at the Heart of Sugar Metabolism: New Mechanisms and Novel Discoveries
… regulate gene expression. Specifically, various RNA-mediated genetic control elements have been discovered in the past decade through a combination of genetics, bioinformatics, and transcriptomic approaches. Together, these RNA elements control the expression of many genes involved in diverse …
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INVESTIGATING THE COMPETITION BETWEEN COMPONENTS OF DUAL-FUNCTION RNA
Non-coding RNAs (ncRNAs) and small proteins have both emerged as important regulators of gene expression. Dual-function RNAs encode a small protein and have a separate function as a regulatory RNA. Although first discovered in bacteria, dual-function RNAs have now been identified and characterized …
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Redefining endothelial progenitor cells using a proteomics approach
… serve as potential markers for EPCs, an alternative proteomic method was adopted to obtain sufficient membrane material for proteomic analysis. Microparticles (MPs), the intact vesicles formed from the plasma membrane, were harvested from the conditioned medium of EPC cultures and their …
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Outer Membrane Porin Regulation among Different Sequence Types of Escherichia coli Clinical Isolates
… coli sequence types by comparing steady-state mRNA levels, mRNA half-life, small regulatory RNA (sRNA) expression, and protein production. It was hypothesized that the regulation of porin production in clinical isolates of E. coli would involve sRNAs and be dependent upon sequence type, …
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Transposon Mutagenesis Facilitates Discovery Of Genotype-Phenotype Associations and Functional Interrogation of the <i>Mycobacterium Kansasii</i> Genome
… a gene encoding a conserved mycobacterial small regulatory RNA for which we established a previously unrecognized role in (macro)colony morphology and biofilm formation. Additionally, we showed <em>Galleria mellonella</em> larva are susceptible to <em>Mk</em> infection, demonstrating the …
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Mechanistic and physiological insights into post-transcriptional regulation by small RNAs SgrS and DicF
Base pairing small regulatory RNAs (sRNAs) are important post-transcriptional regulators of gene expression in bacteria. These sRNAs deploy novel mechanisms to regulate mRNA targets leading to various physiological outcomes during stress conditions including, but not limited to, iron starvation, …
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The evolution, modifications and interactions of proteins and RNAs
Proteins and RNAs are two of the most versatile macromolecules that carry out almost all functions within living organisms. In this thesis I have explored evolutionary and regulatory aspects of proteins and RNAs by studying their structures, modifications and interactions. In the first chapter of …
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Viengrandines mažąsias RNR modifikuojančių gyvūnų Hen1 metiltransferazių taikymas RNR žymėjimui ir sekoskaitai /
… nucleotide in a subset of small non-coding RNAs. This modification protects small RNAs from degradation, meanwhile the deficiency of the Hen1 protein causes detrimental phenotypic changes to particular organisms. Current work presents the first detailed characterization of the enzymatic …
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A Census of Human RNA-Binding Proteins and Characterization of the DEDDh RNA Exonuclease NEF-sp in the 3' End Maturation of 28S Ribosomal RNA
… acting directly upon coding and non-coding RNAs, regulating and executing their maturation, ribonucleoprotein assembly, transport, stability and translation. RNA-binding proteins (RBPs) and ribonucleoprotein (RNP) complexes coordinate these processes. RBPs are central to cellular metabolism …
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Systems metabolic engineering through application of genome-scale metabolic flux modeling
… to implement metabolic engineering strategies, regulatory RNA molecules (antisense RNAs) were designed to help target desired mRNA molecules in the metabolic network. Thermodynamic binding calculations were found to correlate with the efficiency of asRNA-mRNA binding and inhibition of mRNA …
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