Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 46 for “"Redox regulation"”.
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Redox Regulation of Cell Fate
To further study the intracellular redox environment, two novel technologies were developed: first, a conductive substrate cell culture system to control the intracellular redox environment via electrical potentials; and second, a FRET (fluorescence resonance energy transfer)-based redox biosensor …
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Akt2 Redox-regulation in Breast Cancer and Diabetes
… pathway, the Akt isoforms exhibit divergent regulation of downstream processes. The molecular basis for these differences is unknown. Of the three isoforms, Akt2 was found to be uniquely regulated by hydrogen peroxide (H2O2), an established secondary messenger molecule. Akt2 kinase activity …
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Redox-regulation of starch and lipid synthesis in leaves
Post-translational redox-regulation is a well-known mechanism to regulate enzymes of the Calvin cycle, oxidative pentose phosphate cycle, NADPH export and ATP synthesis in response to light. The aim of the present thesis was to investigate whether a similar mechanism is also regulating carbon …
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Redox Regulation of Type Iα PKA in the Heart
… enhancing their binding. I assessed whether thioredoxin (Trx) redox recycles disulfide RIα back to its reduced state. In adult rat ventricular myocytes (ARVMs) pre-treatment with diamide (inducing disulfide) potentiated isoprenaline-induced cardiac Troponin I phosphorylation. Inhibition of …
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Redox Regulation of Akt Phosphorylation in PTEN-/- Mouse Embryonic Fibroblasts
… the PTEN-independent pathways involved in the regulation of Akt phosphorylation by O2.- in MEFPTEN-/- cells is investigated. Using diphenyleneiodonium chloride (DPI), the inhibitor for the O2.--producing NADPH oxidases and silencing of the Nox4 isoform by small interference RNA, we show that …
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Investigations into the chemistry of protein tyrosine phosphatase redox regulation
… PTPs, therefore, play a central role in the regulation of diverse cellular processes including glucose metabolism, cell cycle control and immune responses. Accordingly, small molecules capable of inactivating PTPs through reversible oxidation of their active site cysteine thiolate may find …
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Redox Regulation of T cell Activation and Function During Viral Infection
… T cell activation and the importance of their regulation during an immune response. ROI generated in response to receptor stimulation play an important role in mediating cellular responses. We have examined the importance of reversible cysteine sulfenic acid formation in naïve CD8+ T cell …
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Direct Redox Regulation of F-Actin Assembly and Disassembly by MICAL
… phosphate (NADPH) in oxidationreduction (Redox) reactions. Although MICALs have no known substrate/s, my in vivo and in vitro results revealed that Mical employs its Redox region to bind F-actin and disassembles filaments in an NADPH-dependent manner. Moreover, this Mical-treated actin …
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Direct Redox Regulation of F-Actin Assembly and Disassembly by MICAL
… phosphate (NADPH) in oxidationreduction (Redox) reactions. Although MICALs have no known substrate/s, my in vivo and in vitro results revealed that Mical employs its Redox region to bind F-actin and disassembles filaments in an NADPH-dependent manner. Moreover, this Mical-treated actin …
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Redox Regulation of Haemostasis; Modulation by Inspiratory Hypoxia and Physical Exercise
… suggests compelling evidence of in-vivo regulation of coagulation by ROS. But the direction of change was completely contrary to the original hypothesis. The confirmation that hypoxia does not activate coagulation is important, especially given the controversy surrounding long-haul flight …
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DNA Repair, Redox Regulation and Modulation of Estrogen Receptor Alpha Mediated Transcription
… protein, Apurinic/apyrimidinic endonuclease 1 or redox factor-1 (Ape1/Ref-1), interacts with ERalpha, promotes the ERalpha-ERE interaction, influences ERalpha-mediated transactivation, and selectively associates with endogenous, estrogen-responsive genes in MCF-7 cells. Our findings suggest that …
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REDOX REGULATION OF B CELL ACTIVATION AND THE ANTI-VIRAL HUMORAL IMMUNE RESPONSE
Reactive oxygen intermediates (ROI) are often regarded as toxic byproducts whose uncontrolled production induces oxidative stress damaging macromolecules and have been implicated in a wide variety of disorders. However, recent studies suggest that ROI facilitate normal cellular physiology by acting …
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Ribonucleotide reduction and redox regulation in Archaea : surprising twists on a common theme
… dual substrate selectivity. Our investigation of redox regulation focused on thioredoxin reductase (TrR) and thioredoxin (Tr). In addition to providing the reducing equivalents for many RNRs, TrRs are involved in maintaining the intracellular redox environment. This process typically involves the …
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The redox regulation of alpha 5 beta 1 integrin stimulated MMP production in articular chondrocytes
Elevated levels of reactive oxygen species (ROS) have been observed in the cartilage of osteoarthritis (OA) patients and may contribute to the degradation of the articular cartilage extracellular matrix (ECM). ROS have been shown to serve as secondary messengers in integrin and cytokine signaling …
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Redox regulation of vascular NO bioavailability during hypoxia: implications for oxygen transport and exercise performance
The reduction in O2peak at altitude is well documented. Maximal exercise in hypoxia is accelerated through a reduction in O2 supply with contributions from central and peripheral origins of fatigue. Changes in cerebral and muscle oxygenation have not been well characterised during incremental …
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AN INVESTIGATION OF THE REDOX REGULATION OF THE DEOXYNUCLEOTIDE TRIPHOSPHOHYDROLASE SAMHD1 AND ITS ROLE IN NUCLEOTIDE HOMEOSTASIS
The purpose of this study was to investigate the regulation of SAMHD1 and its relation to nucleotide metabolism and cell cycle progression. Phosphorylation of SAMHD1 is the most extensively documented means of regulation. While questions still remain regarding regulation of SAMHD1 by …
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Computational modeling of the IL-4 pathway to understand principles of systemic redox regulation in cell signaling
… Therefore, a mechanistic basis of ROS-mediated regulation of cell signaling must be established to enable rational design of antioxidant-based therapies. The challenges in quantification of transient changes mediated by ROS during cell signaling have impeded investigation of redox-regulated …
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Effects of Changes of Redox Properties of the Chloroplast ATP Synthase in Arabidopsis
… to serine substitutions caused loss of the redox regulation and the ATP synthases were locked in reduced configuration. The ""permanently reduced"" ATP synthases could not be fully deactivated in the dark. There was no significant difference in photosynthesis rate and growth rate suggesting …
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UNDERSTANDING SULFUR BASED REDOX BIOLOGY THROUGH ADVANCEMENTS IN CHEMICAL BIOLOGY
… formation is crucial to understanding cellular redox regulation.
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Broccoli Components as Antioxidants: The Role of Isothiocyanates as Redox Modifiers via Glutathiolation
… of isothiocyanate-induced oxidative stress in redox regulation by glutathiolation has yet to be evaluated. The objective of this project was to elucidate the role of broccoli components in antioxidant activity, both direct and indirect. Using 22 broccoli genotypes, the variation in …
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