Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 4 of 4 for “"Recombinase-mediated cassette exchange"”.
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A Genetic Test of a Model for Two Activities of Fushi Tarazu Protein in Drosophila Melanogaster
… alleles for intragenic complementation. CRISPR-mediated homology directed repair (HDR) was used to introduce engineered ftz alleles into the ftz locus. Subsequently, four ftz engineered alleles were constructed in vectors for reintroduction by Recombinase-mediated cassette exchange (RMCE). …
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Developing a novel Drosophila model to study Menin biological function
… Mnn1 isoform (C-08), produced by CRISPR/Cas9-mediated insertion via the ExTaSy recombinase-mediated cassette exchange system. Anti-HA immunofluorescence revealed that endogenous Menin is broadly expressed across the larval central nervous system with predominantly nuclear localisation and is …
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Artificial neural network and precision genome engineering frameworks for genetic system engineering in mammalian cells
… biology. The genome engineering framework is a recombinase-mediated cassette exchange (RMCE) landing pad. I apply this framework to address challenges associated with organoid engineering, such as the lack of ability to reliably engineer organoid formation and maturation. In particular, I …
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Investigating "difficult-to-express" monoclonal antibody frameworks in transient and site-specific integration-based Chinese hamster ovary cell expression systems
… of these regulatory elements in mAb expression cassettes increased productivity up to 7 – 11-fold across conditions by simultaneously increasing transcription and modulating the ratio of heavy chain and light chain transcripts. Finally, we used this optimized SSI system to systematically compare …