Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 5 of 5 for “"Recombinase polymerase amplification (RPA)"”.
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Development of a Multi-Component Biosensor for Recombinase Polymerase Amplification-CRISPR/Cas12a-Based Detection of Fusarium oxysporum f. sp. cubense tropical race 4
… combines microneedle-based DNA extraction, Recombinase Polymerase Amplification (RPA), Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas12a-mediated detection, and electrochemical square wave voltammetry (SWV) with targeted integration into a microfluidic system for …
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Isothermal Nucleic Acid Assays for the Detection of HIV Drug Resistance and Sickle Cell Disease in Low-Resource Settings
… transcriptase is isothermally amplified using a recombinase polymerase amplification (RPA) assay. Next, an oligonucleotide ligation assay (OLA) is used to selectively label the mutant and wild type amplified sequences. Finally, a lateral flow ELISA differentiates between OLA-labeled products with …
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Development of a rapid molecular method for detection of C. perfringens and other pathogens in water sources
… methods. Molecular tests using nucleic amplification and detection via the polymerase chain reaction (PCR) method needs thermal cycling instruments and thus is limited in resource-poor environments and spaces lacking infrastructure such as electricity. A solution is to use isothermal …
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Immunological and Molecular Techniques for Point-of-Care Diagnostics
… buffer. Also, a panel of rapid assays based on Recombinase Polymerase Amplification, an isothermal nucleic acid amplification technique, were developed for the detection of urinary tract infection (UTI) causing bacteria. The five assays developed as part of this panel detected 100 genomes per …
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Novel approaches to the low-cost, portable and rapid detection of bacterial pathogens in foods and food-processing environments
… has been previously combined with real-time polymerase chain reaction (qPCR) for the rapid detection of E. coli. While use of qPCR obviates the need for time-consuming steps such as gel electrophoresis, its inherent complexity and cost may prohibit its use in point-of-care or resource-limited …