Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"Recombinase Polymerase Amplification"”.
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RAPID DETECTION OF THE IDH1 R132H MUTATION IN GLIOMAS USING RECOMBINASE POLYMERASE AMPLIFICATION
… an assay to detect the IDH1-R132H mutation using recombinase polymerase amplification in 8 minutes. Our assay is practical for cancer diagnostics because of its simplicity, specificity, and sensitivity. The isothermal nature of the reaction adds to its simplicity. We have designed highly specific …
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Development of a Multi-Component Biosensor for Recombinase Polymerase Amplification-CRISPR/Cas12a-Based Detection of Fusarium oxysporum f. sp. cubense tropical race 4
… combines microneedle-based DNA extraction, Recombinase Polymerase Amplification (RPA), Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas12a-mediated detection, and electrochemical square wave voltammetry (SWV) with targeted integration into a microfluidic system for …
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Development of a Smartphone Based Point of Care Device for Multiplexed Infectious Disease Diagnosis
… capabilities. Quantum dot microbead barcoding, Recombinase Polymerase Amplification and fluorescent microscopy are three technological platforms that have been shown through the work of Chan et al. to be ideal for multiplexed point of care detection when integrated with a smartphone. When …
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Investigation and application of methods for ancient DNA research
… long-range PCR amplicons, b) demonstrates that recombinase polymerase amplification is a less biased alternative to PCR in hybridization capture of aDNA, c) develops an analytical approach that improves phylogenies generated with data from the Illumina BovineSNP50 BeadChip (a commercially …
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Immunological and Molecular Techniques for Point-of-Care Diagnostics
… buffer. Also, a panel of rapid assays based on Recombinase Polymerase Amplification, an isothermal nucleic acid amplification technique, were developed for the detection of urinary tract infection (UTI) causing bacteria. The five assays developed as part of this panel detected 100 genomes per …
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Isothermal Nucleic Acid Assays for the Detection of HIV Drug Resistance and Sickle Cell Disease in Low-Resource Settings
… transcriptase is isothermally amplified using a recombinase polymerase amplification (RPA) assay. Next, an oligonucleotide ligation assay (OLA) is used to selectively label the mutant and wild type amplified sequences. Finally, a lateral flow ELISA differentiates between OLA-labeled products with …
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Development of a rapid molecular method for detection of C. perfringens and other pathogens in water sources
… methods. Molecular tests using nucleic amplification and detection via the polymerase chain reaction (PCR) method needs thermal cycling instruments and thus is limited in resource-poor environments and spaces lacking infrastructure such as electricity. A solution is to use isothermal …
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Novel approaches to the low-cost, portable and rapid detection of bacterial pathogens in foods and food-processing environments
… has been previously combined with real-time polymerase chain reaction (qPCR) for the rapid detection of E. coli. While use of qPCR obviates the need for time-consuming steps such as gel electrophoresis, its inherent complexity and cost may prohibit its use in point-of-care or resource-limited …