Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 59 for “"Recombinants"”.
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Small RNA and genome interactions in Chlamydomonas reinhardtii recombinants
… both the genomes and sRNAomes of parents and recombinants, I was able catalogue both genetic and epigenetic variation in the parental strains providing unique insight into the inheritance of small RNAs in this alga. In this thesis, I first compare the genomes of the parental strains, …
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Generation of Baculovirus-Brucella Abortus Heat Shock Protein Recombinants; Mice Immune Responses Against the Recombinants, and B. Abortus Superoxide Dismutase and L7/L12 Recombinant Proteins
… proteins is currently unresolved. Baculovirus recombinants were generated in <i>Sf9</i> insect cells for <i>B. abortus</i> HSPs and the protein expression was optimized. Humoral (Western blot), cell mediated (CMI, IFN-g- release by splenocytes, and CD3+CD4+, CD3+CD8+ T cell/ total splenocytes …
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Non-parental-type Recombinants in Crosses between Different Restriction-Modification Systems in SALMONELLA
… In PI transduction studies with these systems, recombinants were isolated which fell into one of three classes with reference to their specific restriction phenotype. Recombinants that restricted like the donor, recombinants that restricted like the recipient and recombinants that had lost the …
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Induction of Protection, Antibodies and Cell Mediated Immune Responses by Brucella Abortus Strain RB51, Ochrobactrum Anthropi and Recombinants Thereof
Although it is known that cell-mediated immunity (CMI) plays a key role in protection against brucellosis, the exact immune mechanisms leading to protection are still not fully understood. Better understanding of the mechanisms would help in the development of a human Brucella vaccine and help in …
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Identification of a chromosomal region possibly involved in O-side chain biosynthesis in Brucella abortus
… and pNW-3) and Brucella chromosomal DNA. The recombinants were checked for their O-side chain by crystal violet uptake and immunoblotting with monoclonal antibody specific for the O-side chain. The locations of Tn5 and the flanking region in the genome of these recombinants were characterized …
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Isolation and Lipid Binding Properties of the Low Molecular Weight Protein Components of Bovine High Density Lipoprotein
… a variety of initial lipid: protein ratios. Recombinants with D(,4) were less discrete, with the size and stoichiometry of complexes being dependent on the initial lipid: protein ratio used. The complexes formed are of a size (2 to 3 x 10('5) daltons) and weight percentage protein composition …
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Tissue-Specific Mediation of Estrogen-Induced Epithelial Events by the Estrogen Receptor in Female Reproductive Tract Organs
… fractions were recombined to produce tissue recombinants that contained ERalpha in both epithelium and stroma or lacked ERalpha in one or both tissue compartments. Recombined tissues were grafted to host animals and grown in vivo. By monitoring the extent of epithelial mitogenesis and …
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Applicability of vaccinia virus as cloning and expression vector for bacterial genes: mice immune responses to vaccinia virus expressing Brucella abortus and Listeria monocytogenes antigens
… studies by our group showed that vaccinia virus recombinants expressing Brucella abortus (BA) antigens heat shock protein GroEL, 18 kDa protein and Cu/Zn SOD, were unable to induce protective immune responses against Brucella challenge. This dissertation analyzes the possible reasons for this …
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Clonage du génome l'herpesvirus cyprin-3 en tant que chromosome artificiel bactérien
… l’utilité du BAC KHV pour la production de recombinants a été démontrée par la production d’une souche délétée pour l’ORF16 grâce au principe de recombinaison en système procaryote. L’obtention du BAC KHV est une avancée importante qui permettra l’étude des gènes viraux impliqués dans la …
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Functional variation in the mitochondrial genome of the yeast saccharomyces cerevisiae
… and likely due to multiple loci. Mitochondrial recombinants showed poor growth in ecologically relevant conditions consistent with negative epistasis between mitochondrial loci (mito-mito epistasis). I generated recombinants between mitotypes from various populations and found significant …
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Μελέτη επιπολασμού υπότυπων και ανασυνδυασμένων τύπων HIV-1 στις περιοχές της Ευρώπης, Αφρικής και Αμερικής, την περίοδο 2007-2024 / Prevalence Study of HIV-1 subtypes and recombinants in Europe, Africa and America, during the period 2007-2024
Εισαγωγή: Ο ιός της ανθρώπινης ανοσοανεπάρκειας (HIV) παρουσιάζει μια πανδημική δυναμική άνω των τεσσάρων δεκαετιών. Διακρίνεται σε δύο τύπους, εκ των οποίων ο τύπος 1 (HIV-1) αποτελεί τον επικρατέστερο παγκοσμίως. Σκοπός: Διερεύνηση των τάσεων του επιπολασμού των HIV-1 υπότυπων και ανασυνδυασμένων …
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Molecular analysis of the salmon pathogen Piscirickettsia salmonis
… appeared to contain approximately 1.79 x 105 recombinants/g DNA, and the recombinant library was screened with rabbit antiserum to purified P. salmonis and antisera raised in fish. No reactive clones were detected in screening with antisera, or with oligonucleotide probes against both P. …
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The role of resistance to varroa destructor and deformed wing virus in the European honey bee (apis mellifera)
… these colonies. Finally, the role of DWV viral recombinants were investigated on a landscape scale using samples from England and Wales, and the USA. This provided further evidence that viral recombinants are not as fit as parent genomes. Together these results deepen our understanding of DWV …
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Evolution of viral populations in individuals infected with single and multiple HIV subtypes : a study of HIV-1 dual infection in a high risk cohort of female bar workers from Tanzania
… population (n=57). Subtypes A, C, D and their recombinants forms were detected. Subtype A accounted for 5%, C 33%, D 7% and recombinants 44%. Dual infection was detected in 11 % of individuals. AC recombinants accounted for 60% of all recombinant viral strains, ACD and CD strains accounted for …
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Food Allergens and allergenicity of glycine max (L.) merr. (soy)
… dehydrogenase) soy allergens were produced as recombinants, and used in an immunoassay to screen 84 soy-specific sera. The three putative allergens were verified to be antigenic, together with four of the known allergens.
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Evaluation of arthropod-borne virus recombination potential and phenotype: In vitro and in vivo analysis of flavivirus and alphavirus model systems
… the potential for the emergence of virulent recombinants either post-vaccination or following co-infection with two distinct wild-type viruses. In order to characterize the conditions and sequences that favor RNA arthropod-borne virus recombination I designed, constructed, and evaluated …
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Design of a Tetracycline Operon Inducible System for the Control of Vaccinia Virus Replication: Implications for Vaccine Development
… elements. Of the seven VACV genes tested, recombinants utilizing the A6L, A7L, D6R, and F17R genes were successful in regulating viral replication with tetracyclines.</p>
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Utilising machine learning techniques on simulated viral evolution datasets to improve viral recombinant identification
… demonstrated modest performance in detecting recombinants (precision 0.627-0.687, recall 0.652-0.686). These improvements, though incremental, represent progress in automated recombination detection. The successful preliminary integration of the logistic regression model into RDP5 demonstrates …
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Physical Map between Marker 8O7 and 146O17 on the Medicago truncatula Linkage Group 1 that Contains the NIP Gene
… on M. truncatula Linkage Group 1. Informative recombinants showed crossovers that localize the NIP gene between markers 146O17 and 23C16D. Marker 164N9 co-segregates with the NIP gene, and the location of marker 164N9 is between markers 146O17 and 23C16D. Based upon data from the Medicago …
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