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Showing 1 to 20 of 180 for “"Recombinant proteins"”.
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Purification of recombinant proteins
Thesis (M.S.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 1997.
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Recombinant Proteins for Biomedical Applications
… leaps in areas such nucleic acid, antibody, and recombinant protein technologies. Here we focus on the use of recombinant proteins as molecular recognition motifs, wound healing biomaterials, and agents for cell cycle pathway elucidation are discussed. The author's primary project is described in …
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Purification of recombinant proteins with magnetic nanoclusters
… a new class of magnetic fluids for recovery of recombinant proteins from fermentation broth. Magnetic fluids are colloidally stable dispersions of magnetic nanoclusters in water that do not settle gravitational and moderate magnetic fields due to their small size. The magnetic nanoclusters …
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Oxidative damage to recombinant proteins during production
Since the introduction of recombinant human insulin nearly two decades ago, recombinant proteins have increasingly been utilized as therapeutic agents. In addition, expression of recombinant proteins is now a common tool used in basic research. Recombinant proteins are subject to many subtle …
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Mechanistic studies on chemical instabilities of recombinant proteins
Protein molecules are being widely used as pharmaceuticals for treating diseases ranging from diabetes and haemophilia to various types of cancers due to their great potency and specificity. However, these macromolecules are intrinsically unstable in aqueous solutions, due to the existence of …
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Bacterial expression of radio-labeled recombinant proteins for studying AHR signalling
… to AHR and ARNT there are at least three other proteins involved in AHR signaling. These proteins are the co-chaperone p23, Ara-9 and two molecules of Heat Shock Protein-90 (HSP-90). This study documents the production of Ara-9 and C∆418 (an ARNT deletion construct) in a modified thioredoxin …
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Construction of an efficient secretion system for recombinant proteins in Bacillus subtilis
All proteins being translocated through the cytoplasmic membrane of bacteria cells as well as some proteins that are inserted into the cytoplasmic membrane contain a signal sequence at their N-terminus that is recognized by and targeted to the translocation machinery. Three translocation pathways …
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Characterization of components that increase secretion of recombinant proteins in pichia pastoris
… its ability to express and secrete heterologous proteins. However, some proteins are not readily secreted in P pastoris and so adjustments in the secretion pathway must be made in order to achieve secretion. The Lin-Cereghino lab previously developed mutant strains using restriction …
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Presentation of Recombinant Proteins in Modified Vaccinia Virus Ankara Extracellular Enveloped Virions
… the smallpox eradication campaign. Nowadays recombinant attenuated poxviruses gain importance as live carrier vaccines against different infectious diseases and in cancer therapy. The aim of this work was to develop recombinant viral vectors, for presentation of a foreign antigen on the …
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Process-scale renaturation of recombinant proteins from inclusion bodies / by Nicholas Kotlarski.
Scale-up of a biochemical process involving expression of an Insulin-like Growth Factor-I analogue (LongR3IGF-I) as inclusion bodies within the bacterium Escherichia coli has been investigated. The principal focus was directed to the operation of refolding wherein the biological potency of the …
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Characterizing potential secretion components that increase secretion of recombinant proteins in Pichia Pastoris
… to produce and readily secrete heterologous proteins. Nonetheless, there are obstacles in making this useful yeast into a more efficient secretion system that readily secretes problem proteins. In the Lin-Cereghino lab, mutant strains were developed by the method of restriction enzyme …
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Characterizing phenotypes of Pichia pastoris mutants that show enhanced secretion of recombinant proteins
… ability to produce and secrete various types of recombinant proteins. The mutants showed various population doubling times, which ranged from 1.7 to 2.4 hours. Generally, the mutants with severely diminished growth rates had much lower secretion of the reporter proteins. The mutants also showed …
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Engineering functional recombinant proteins based on antibody domains and fragments of C. botulinum neurotoxin
… variable) and single domain antibodies. Recombinant botulinum neurotoxin fragments derived from serotype A, B, C and D are selected as backbones for an investigation into the feasibility and properties of novel recombinant targeted multidomain proteins. Single chain Fv’s consist of a VH …
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Identification of MRSA-specific peptides and expression of peptide containing recombinant proteins for targeted photodynamic therapy
… format, into an E. coli based expression vector. Recombinant protein expression was optimised and the protein extracted, purified and characterised by Western blotting. The porphyrin photosensitiser (5-(4-isothiocyanatophenyl)-10,15,20-tris-(4-N-methylpyridiniumyl) porphyrin trichloride) was …
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Generation of Baculovirus-Brucella Abortus Heat Shock Protein Recombinants; Mice Immune Responses Against the Recombinants, and B. Abortus Superoxide Dismutase and L7/L12 Recombinant Proteins
… in macrophages by producing heat shock proteins (HSPs) which are induced by environmental stresses. Bacterial HSPs are very immunogenic, eliciting both cellular and humoral immune responses in the infected host. The significance of host cellular and protective immune responses directed …
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The study of cell surface components on Porphyromonas gingivalis
… raised to P. gingivalis whole cells. The recombinant clones Escherichia coli BA1 and E. coli BA3 were identified and chosen for further study. E. coli BA1 exhibited enhanced haemagglutinating activity compared to an E. coliJM83/pUC18 control strain. The increase in haemagglutinating …
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Mammalian Cell Line Development Platform for Recombinant Protein Production: Expanding the Protein Expression Toolbox for Research and Drug Discovery Applications
<p>Recombinant proteins have revolutionized the biomedical industry, providing therapeutics for life-threatening diseases and protein reagents for research applications. BioMarin Pharmaceutical Inc. develops recombinant protein therapeutics to treat rare diseases including lysosomal storage …
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Characterication of aggregate gland silk factor 1
… We also overexpress and purify two different recombinant AgSF1 fusion proteins, named AgSF1G and AgSF1G+GXPXP. These recombinant proteins encompass different regions within the AgSF1 amino acid sequence. Using wet-spinning methodology we also demonstrate that these proteins can be spun into …
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Engineering and Characterization of a Single Chain Antibody Specific for Murine CD28
… favorably to other methods relying on soluble proteins and will facilitate the engineering and characterization recombinant proteins in the future.
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