Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"Recombinant protein production"”.
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Physiological aspects underpinning recombinant protein production in Escherichia coli
Many biopharmaceutical projects require the production of recombinant protein in a bacterial host. Conventional procedures used for recombinant protein production (RPP) involve the rapid synthesis of the target protein. This results in the accumulation of unfolded protein, the induction of the heat …
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A directed evolution approach to engineering recombinant protein production in S. cerevisiae
The continued success of protein therapeutics has put a strain on industry's ability to meet the large demand. Creating a more productive expression host for the manufacture of these proteins is a potential solution. Although heterologous proteins are frequently made in organisms as disparate as E. …
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Recombinant Protein Production in Bacillus Species, Isolation and Methionine Production in Methionine-producing Microorganisms
… in developing commercial processes for the production of single isomers of CLA by biotransformation of LA using microbial cells and enzymes. However, the evaluation of these strains suggest that growth and LA isomerase production levels by these anaerobes are insufficient to support economic …
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Active hypothermic growth : a novel means for increasing total recombinant protein production by CHO cells
Recombinant human glycoproteins produced by Chinese Hamster Ovary (CHO) cells are an important class of therapeutic molecules and investigating means of improving the production rate and product quality of these glycoproteins is therefore of great interest. Culturing CHO cells under mild …
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Application of metabolic and biochemical engineering techniques for the enhancement of recombinant protein production in Escherichia coli
… acetate excretion, as a means to enhance recombinant protein productivities. The recombinant model system is based on the formation of plasmid encoded cadA-LacZ fusion protein with $\beta$-Galactosidase activity under the control of the pH-regulated promoter. This recently developed …
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Lactogenesis Induction in Transgenic Virgin Pigs as a Model for Identifying Transgene Expression and Recombinant Protein Production
The porcine mammary gland can be used for the production of recombinant proteins by directing a transgene to the mammary gland with a milk protein gene promoter. In order to determine whether or not the protein will be expressed, the animals must be maintained at least through their first …
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Mammalian Cell Line Development Platform for Recombinant Protein Production: Expanding the Protein Expression Toolbox for Research and Drug Discovery Applications
<p>Recombinant proteins have revolutionized the biomedical industry, providing therapeutics for life-threatening diseases and protein reagents for research applications. BioMarin Pharmaceutical Inc. develops recombinant protein therapeutics to treat rare diseases including lysosomal storage …
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Global transcriptional analysis of an Escherichia coli recombinant protein process during hypoxia and hyperoxia
(cont.) The effects of recombinant protein production were observed through expression analysis of induced, uninduced, and Empty-Vector cultures. As expected, recombinant α₁AT production led to increased expression of heat-shock genes, including proteases and chaperones that are known to be …
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Expression of Nanobodies in Marchantia polymorpha chloroplasts
The production of recombinant proteins, such as antibodies and nanobodies, is essential for biomedical and other practical applications. Plants possess many advantages for recombinant protein production, including lower costs, larger scale production compared to other systems and low risk of …
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Increasing IFN-[gamma] productivity in CHO cells through CDK inhibition
Approximately 60-70% of all recombinant human glycoproteins are produced in Chinese Hamster Ovary (CHO) cells. Production in CHO cells, however, is often plagued by low productivity when compared with other host cell lines, including bacteria and yeast. For this reason, investigating ways of …
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Influência das condições de cultivo na produção de antígenos recombinantes de Leishmania i. chagasi utilizando Escherichia coli M15 cultivada em incubador rotativo e biorreator
… has been one of the most widely used hosts in recombinant protein production, in both laboratory and industrial scale since the advent of recombinant DNA technology. Despite the substantial progress of studies on the molecular biology and immunology of infections, there is currently no …
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The co-modulation of apoptosis and the cell cycle
… reliant on mammalian cell culture for the production of biopharmaceuticals; therefore research aimed at improving the characteristics of the cell-lines being used for recombinant protein production is essential. In this study I have examined the hypothesis that the creation of a CHO …
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High-throughput tools for decoding T cell receptor specificity
… by major histocompatibility complex (pMHC) proteins, driving immune responses in cancer, infection, and autoimmunity. Understanding how TCRs recognize antigens is crucial for developing cancer immunotherapies and identifying therapeutic targets in autoimmunity, infectious disease, and …
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Upstream and downstream issues in the production of recombinant vitamin K-dependent proteins in the milk of transgenic pigs
… safety concerns for plasma-derived therapeutic proteins, such as protein C and Factor IX, have spurred the development of recombinant DNA technology for large-scale, economically feasible production of therapeutic proteins. The mammary gland is unique in its ability to synthesize and secrete …
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Printing protein-engineered paper for biosensors
… of the paper properties and reduce the cost of production and immobilisation of bioreagents onto paper. This thesis explores whether introducing digital printing into papermaking in combination with the recombinant protein production of paper-binding bioreagents could provide an accessible, …
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Plant production of Gonococcal peptide vaccine, candidate peptide display with HPVs
… explores the innovative approach of plant-based production for a dual vaccine against HPV16 and gonorrhea, utilizing N. benthamiana as the expression host. The vaccine design involves the incorporation of a gonorrhea peptide, mimicking the 2C7 epitope of the surface molecule LOS on gonorrhea, …
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The effect of dissolved oxygen on recombinant protein degradation in Escherichia coli
The production of proteins in recombinant host cells has become an invaluable tool to obtain significant amounts of protein for therapeutic and other applications. Efforts to increase expression of foreign proteins in Escherichia coli, however, put an increasing burden on protein synthesis and …
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Maintaining the balance: persistent baculovirus infection in insect cells
… cultures. Presence of BV and the major membrane protein GP64 confirmed C20 harboured a persistent rather than latent infection. Analysis indicated that only a small proportion (<7%) of cells were producing BV whilst superinfection studies demonstrated that the majority of cells were resistant to …
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Assessment of potential antibacterial drug targets
… Once ten different targets were selected, recombinant protein production was carried out to support structural and biochemical characterisation. Seven proteins were successfully purified and four of them prioritised for further studies. These are kynurenine formamidase (KynB), …
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Applications of yeast display in synthetic biology with a focus on biological synthesis and recycling of plastics
… tool in synthetic biology, particularly in protein engineering. This work sought to improve the flexibility and ease-of-use of yeast display for synthetic biologists, as well as applying the yeast display system in several contexts – with a particular focus on biological synthesis and …
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