Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 192 for “"Recombinant protein"”.
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Overcoming long-standing challenges in recombinant protein expression
<p>The market for proteins has been constantly increasing for many different applications, such as protein-based drugs. However, protein production in E. coli, the most widely used recombinant host, has remained fundamentally the same since its inception. Here, we developed from the bottom up, a …
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Physiological aspects underpinning recombinant protein production in Escherichia coli
… projects require the production of recombinant protein in a bacterial host. Conventional procedures used for recombinant protein production (RPP) involve the rapid synthesis of the target protein. This results in the accumulation of unfolded protein, the induction of the heat shock …
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Purification of an acidic recombinant protein from transgenic tobacco
Tobacco has been studied as a host for producing recombinant therapeutic proteins on a large-scale, commercial basis. However, the proteins expressed in tobacco usually need to be purified to high yield and purity from large amounts of biomass in order for their production to be commercially …
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THERMOSTABLE EXOSHELLS AS TOOLS FOR RECOMBINANT PROTEIN EXPRESSION AND IN VIVO DELIVERY
We have engineered a thermostable protein nanoparticle (tES) to improve both expression and stabilization of recombinant proteins using a single technology. tES provides steric accommodation and charge complementation to GFPuv, horseradish peroxidase (HRP), and Renilla luciferase, and Photinus …
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A directed evolution approach to engineering recombinant protein production in S. cerevisiae
The continued success of protein therapeutics has put a strain on industry's ability to meet the large demand. Creating a more productive expression host for the manufacture of these proteins is a potential solution. Although heterologous proteins are frequently made in organisms as disparate as E. …
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The effect of dissolved oxygen on recombinant protein degradation in Escherichia coli
The production of proteins in recombinant host cells has become an invaluable tool to obtain significant amounts of protein for therapeutic and other applications. Efforts to increase expression of foreign proteins in Escherichia coli, however, put an increasing burden on protein synthesis and …
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Novel recombinant protein constructs for improved insulin-like growth factor-1 delivery
Four novel recombinant protein constructs were designed to improve the delivery of insulin-like growth factor-1 (IGF-1). The IGF-1 sequence was subcloned into a HisMax plasmid and expressed at low levels by transient transfection in 293 cells. Site-specific mutagenesis was used to insert all four …
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Evaluation of drying technologies for storage and shipment of recombinant protein drug substance
… using economically feasible methods that protect protein integrity. Adequate storage and shipment of drug substance is an important operation and product quality issues are dependent upon success at this stage of the manufacturing process. While cryopreservation technologies are widely in use …
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Recombinant Protein Production in Bacillus Species, Isolation and Methionine Production in Methionine-producing Microorganisms
… and to generate new production strains using recombinant technology. <em>Bifidobacterium<em> LAI genes were cloned into pET24a(+) as an expression vector and transferred into <em>E. coli<em> BL21 (DE3) as the expression host while <em>Propionibacterium acnes<em> LAI were cloned into …
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Global transcriptional analysis of an Escherichia coli recombinant protein process during hypoxia and hyperoxia
(cont.) The effects of recombinant protein production were observed through expression analysis of induced, uninduced, and Empty-Vector cultures. As expected, recombinant α₁AT production led to increased expression of heat-shock genes, including proteases and chaperones that are known to be …
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The effects of cis- and trans-acting mutations on recombinant protein secretion in Pichia pastoris
… in both research and industrial settings for recombinant protein expression due to the ease of genetically modifying its genome, its ability to grow to large densities in inexpensive media, and its capability to perform posttranslational modifications. Multiple tools such as the cis -acting …
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Active hypothermic growth : a novel means for increasing total recombinant protein production by CHO cells
Recombinant human glycoproteins produced by Chinese Hamster Ovary (CHO) cells are an important class of therapeutic molecules and investigating means of improving the production rate and product quality of these glycoproteins is therefore of great interest. Culturing CHO cells under mild …
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Molecular cloning, recombinant protein expression and structural characterization of a universal stress G4LZI3 protein from Schistosoma mansoni
… has been predicted to encode different stress proteins including the Universal Stress Protein (USP). It has been previously hypothesized that the USP of schistosome parasites have shared protein sequence features which can be useful for inferring their biochemical and environmental regulation. …
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Application of metabolic and biochemical engineering techniques for the enhancement of recombinant protein production in Escherichia coli
… acetate excretion, as a means to enhance recombinant protein productivities. The recombinant model system is based on the formation of plasmid encoded cadA-LacZ fusion protein with $\beta$-Galactosidase activity under the control of the pH-regulated promoter. This recently developed …
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Lactogenesis Induction in Transgenic Virgin Pigs as a Model for Identifying Transgene Expression and Recombinant Protein Production
… mammary gland can be used for the production of recombinant proteins by directing a transgene to the mammary gland with a milk protein gene promoter. In order to determine whether or not the protein will be expressed, the animals must be maintained at least through their first lactation. An …
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Developability assessments and analytical characterization of recombinant protein antigens formulated as low-cost, multi-dose, aluminum-adjuvanted vaccines
… towards developing vaccines consisting of recombinant subunit proteins antigens. This newer approach not only offers advantages of improved safety and efficacy, but also enhance manufacturing scalability and lower costs compared to traditional approaches. This Ph.D. dissertation work …
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The structural characterization of the Saccharomyces cerevisiae alpha mating factor secretion signal for recombinant protein secretion in Pichia pastoris
… has been used extensively for expressing recombinant proteins because it combines the ease of genetic manipulation with rapid growth to high cell densities and provides complex posttranslational modifications. The most successful and commonly used secretion signal leader in Pichia pastoris …
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SELECTION/CONSTRUCTION AND CHARACTERIZATION OF NOVEL PEPTIDE AND RECOMBINANT PROTEIN AGENTS TARGETING INTERLEUKIN 13 RECEPTOR ALPHA 2 IN GLIOBLASTOMA MULTIFORME
Glioblastoma multiforme (GBM) is a primary brain tumor of dismal prognosis. Patients with GBM have a median survival rate of 14.5 months inspite of multimodality treatment involving surgery, radio- and chemotherapy. This warrants an intensive search for efficient new therapies. Interleukin 13 …
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