Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"Recombinant antibodies"”.
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CHARACTERIZATION OF DENGUE NEUTRALIZING ANTIBODIES USING RECOMBINANT ANTIBODIES TECHNOLOGY
… EXPRESSION, AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES OBTAINED FROM TWO NATURALLY INFECTED DENGUE PATIENTS. THIS LED TO THE SUBSEQUENT DISCOVERY AND FURTHER CHARACTERIZATION OF AN ANTIBODY THAT POSSESSES POTENT NEUTRALIZATION CAPACITY AGAINST ALL FOUR DENGUE VIRUS SEROTYPES. IN PARALLEL, AN …
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Recombinant antibodies for the study of livestock infection: from basic genetics to single-chain Fvs
… information for the construction of specific antibodies against a wide variety of antigens including the pathogens of humans and animals. In spite of the economic importance of cattle, many aspects of the immunology of this animal remain uncharacterised and tools to understand better bovine …
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Generation and characterisation of plant produced recombinant antibodies specific to LHRH for treatment of sex hormone dependent diseases
… LHRH analogues or by neutralising anti-LHRH antibodies. While the LHRH analogues require frequent dose administration and are expensive, active vaccination against LHRH is limited by the variation in the immune response from patient to patient and the lag period in the build-up of effective …
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Molecular intervention in Pierce's Disease
… on the X. fastidiosa surface. Blocking MopB with antibodies may prevent or reduce transmission of the pathogen. The main goal of this research was to generate recombinant antibodies to be used to investigate the interaction of MopB with the plant and GWSS. Towards this goal, recombinant MopB has …
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Development of a Versatile Antibody Cloning and Expression System
Over the last three decades, recombinant monoclonal antibodies (mAbs) have become the key tool for basic research, diagnosis and treatment of human diseases. This has required the selection of recombinant antibodies with high affinity for appropriate epitopes on the target antigen and other …
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Exploiting novel antibodies for the early detection of cardiac disease
… a specific targeting strategy, a panel of novel antibodies were successfully generated against key epitopes in cTnI that are of paramount importance for specific cardiovascular disease detection. Panels of both monoclonal and recombinant antibodies were generated and fully characterised. This …
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Antibody phage-displayed libraries derived from chicken immunoglobulin genes : a source of highly specific diagnostic antibodies
In meeting the high demand for monoclonal antibodies, the chicken immunoglobulin system was exploited to generate recombinant antibodies against multiple target antigens. Following simultaneous immunisation of two chickens with a mixture of Plasmodium falciparum recombinant lactate dehydrogenase …
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Antikörper vermittelte Fusarien-Resistenz in transgenen Pflanzen
… and the consumers as well as costs. The use of recombinant antibodies in transgenic plant to generate fungal pathogen resistant plants is a promising way to solve these problems in a consumer-friendly way. The goal of this work was to generate scFv antibody fragments against surface structures …
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Generierung, Charakterisierung und funktionaler Assay von Antikörpern und Antikörperfragmenten gegen die HCV Strukturproteine core, E1 und E2
… carcinoma. In the dissertation on hand, antibodies against the Hepatitis C (subtype 1b) structure proteins core, E1 and E2 were synthesised. The coding sequences of full-length and deleted versions of the protein mentioned above served as starting material. In a first step sufficient …
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Development of high throughput screening systems for the efficient production of antibody fragments in Escherichia coli
Recombinant antibodies and antibody fragments have become powerful tools for therapy, in vivo and in vitro diagnostics, and laboratory research. However, the production of antibody fragments in high yield for preclinical and clinical trials can be a serious bottleneck in drug discovery. This …
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Engineering of recombatinant antimalria antibodies for application in paratransgenesis
… symbionts to express anti-parasite molecules. Recombinant antibodies that target specific antigens expressed on the parasites’ surface could be used as anti-parasite molecules, especially if they could not only bind but agglutinate the target. The murine antibody 4B7 binds to Pfs25 epitope …
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Engineering and characterization of single chain antibody fragments (scFvs) specific to key enzymes in polyamine biosynthesis and manipulation of polyamine pathway by constitutive expression of recombinant ODC and SDE enzymes in transgenic tobacco
… as a target for generation of specific scFv recombinant antibody fragments by phage display technology. Mice were immunized with the bacterial produced and affinity purified ADC, ODC-GST fusion protein and PAO-GST inclusion bodies. High antibody titers showed successful immunization. Three …
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Identifikation und Charakterisierung neuer immunogener Proteine und anschließende Generierung rekombinanter Antikörper mittels Phage Display
Seit der Einführung von Antibiotika in die medizinische Behandlung von bakteriellen Infektionskrankheiten existiert ein Wettlauf zwischen der Evolution von Bakterienresistenzen und der Entwicklung wirksamer Antibiotika. Während bis in die 80er Jahre verstärkt an neuen Antibiotika geforscht wurde, …