Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"Recombinant Fusion Proteins"”.
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Streptavidin-cytokine fusion proteins for use as adjuvants in cancer vaccines
… describes the production and characterisation of recombinant streptavidin-cytokine fusion proteins. Immunoadjuvants can augment the weak or non-existent antitumour response of a patient with cancer to immunotherapy. The response of breast cancer patients to cancer vaccines directed against tumour …
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Establishment of recombinant antibody technologies allowing for the generation of SNAP-tag fusion proteins
… have provided a means of developing homogenised recombinant immunotherapeutics. Snaptag is a modified version of a human DNA repair enzyme, O6 - alkylguanine-DNA-alkyltransferase (AGT) which naturally removes alkyl residues from damaged DNA. The enzyme reacts specifically with benzylguanine (BG) …
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Characterization of the Non-Proteolytic Mechanism and Cellular Site of Action of PCSK9-Mediated Degradation of the Low-Density Lipoprotein Receptor
… the PCSK9:LDLR interaction at the cell surface. Recombinant LDLR subfragments were synthesized and added to the medium of cells that overexpressed PCSK9. These sub-fragments restored LDLRs to levels found in the control cells. These experiments confirmed that the disruption of PCSK9:LDLR at the …
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R-RAS41A fusion protein purification and the cloning of a new RAS GEF like gene
This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).
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Novel cell-penetrating peptide-based vectors for siRNA delivery
… aimed to design, purify and characterize novel recombinant fusion proteins, that would have broad applicability as carrier molecules for non-covalent siRNA delivery. The fusion proteins were comprised of a cell penetrating peptide sequence (either AntpHD, Penetratin, HIV-Tat or EB1) fused to …
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Glutathione metabolism of Plasmodium falciparum
… the localization of γGCS and GS, GFP-tagged recombinant fusion proteins were expressed in the parasites and showed that GSH biosynthesis is cytosolic. Apart form its other functions GSH has previously been suggested to be involved in resistance to the antimalarial drug chloroquine (CQ). CQ …
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Antigen-specific depletion of autoreactive B lymphocytes in Multiple Sclerosis
… In this thesis, novel autoantigen-specific fusion proteins for the targeting and elimination on one hand and the staining of autoreactive B cell populations on the other hand were successfully generated. The membrane-bound B cell receptors of the target cell populations and their specificity …
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Development Of Recombinant Immunotherapeutics for Triple-Negative Breast Cancer (TNBC)
… types. Antibody-Drug-Conjugates (ADCs) are proteins that combine an antibody and a toxin designed to selectively target and kill cancerous cells respectively. These are promising alternatives to conventional cancer treatment. This approach takes advantage of the specific cell surface …
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Ricin B Chain-Insulin Fusion Protein Immunomodulation of Type 1 Diabetes
… human proinsulin (INS) ricin toxin B chain (RTB) fusion protein was expressed in <em>Escherichia coli </em>and purified for use in an animal inoculation experiment to test for the suppression of diabetes symptoms. Non-obese diabetic (NOD) mice were orally inoculated and boosted with purified …
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The Role of TGF-β in CTB-Insulin Regulated Human Dendritic Cell Tolerance
<p>Cholera toxin B subunit fusion to autoantigen vaccines such as proinsulin (CTB-INS) down regulates dendritic cell (DC) activation and induces the synthesis of DC immunosuppressive cytokines. Recent studies of CTB-INS induction of immune tolerance in human DCs indicate that increased IDO1 …