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Showing 1 to 16 of 16 for “"Recombinant Escherichia coli"”.
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Overproduction of xylose isomerase in recombinant Escherichia coli
Aspects of recombinant enzyme production were characterized in genetically engineered Escherichia coli. Five strains transformed with the xylose isomerase overproduction system (pRK248/pTXI-1) were compared, based on parameters of cell metabolism and inducible enzyme activity in shake flask …
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Solution properties and molecular size of polyhydroxybutyrate from recombinant Escherichia coli
Polyhydroxybutyrate molecules produced by recombinant Escherichia coli were evaluated in terms of their size in solution to develop and test a hypothesis concerning the regulation of the molecular weight of PHB during in vivo polymer production. Two recombinant strains of E. coli were used to …
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Process integration in the optimisation of amidase production from recombinant Escherichia coli
… 3.5.1.4) from Geobacillus pallidus RAPc8 using recombinant E.coli BL21 (DE3). The choice of growth medium and induction strategy were optimised under bioreactor conditions to enhance amidase productivity. Further, expanded bed adsorption (EBA) was assessed as a tool for minimising the unit …
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Optimization of Fermentation Conditions for the Production of Legionaminic Acid in Recombinant Escherichia Coli
… In this work, fed-batch cultivations of recombinant Escherichia coli BRL04 were carried out in shake flasks and 5-L bioreactors. The final process was optimized by determining the effects of different carbon sources, induction temperatures, pH, dissolved oxygen (DO) content, induction …
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Improvement of engineered thermostable xylanase production via immobilization of recombinant escherichia coli onto graphene oxide
Escherichia coli is the preference host system for enzyme production by recombinant DNA technology. This was due to its promising trait that is suitable for genetically modify and the obtainability of a difference E. coli strains. But, the main downside when utilizing E. coli as a host is bacterial …
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Investigation of CNT-induced Escherichia coli Lysis and Protein Release
… the permeability of a bacterial cell wall. Recombinant Escherichia coli BL21 (DE3) containing a plasmid that expressed Green Fluorescent Protein (GFP) and -lactamase were exposed to CNTs under various levels of agitation for different times. Fluorescence assay for GFP, optical absorbance …
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Physiological studies on bacterial fermentations using multi-parameter flow cytometry
… this work in order to assess the quality of recombinant Escherichia coli populations present within an agitated bioreactor. It was demonstrated in shake-flask culture that the cells could be grown to moderate cell densities (OD600nm 7 25) whilst producing measurable levels of antibody …
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Engineering of an efficient and enantioselective biocatalyst for the preparation of chiral pharmaceutical intermediates
… stutzeri together with the P450pyr system in a recombinant Escherichia coli.
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Ganzzellbiotransformationen mit rekombinanten Escherichia coli zur Synthese chiraler Alkohole
… addition of external cofactors. In this study recombinant Escherichia coli cells were applied as biocatalysts for the reduction of ketones. By the expression of suitable alcohol dehydrogenases (adh) both enantiomeres of chiral alcohols can be produced. The syntheses of enantiopure …
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Bioproduction Optimization and Characterization of Hyaluronic Acid in Escherichia coli and Nicotiana benthamiana
… microbial and plant-based expression systems. Recombinant Escherichia coli strains were engineered to express human hyaluronic acid synthase 2 (hHAS2) with and without the co-expression of hyaluronidase and were evaluated based on total HA yield and molecular weight. The engineered strains …
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Transcriptional Control during Quorum Sensing by LuxR and LuxR Homologues
… the RNAP alpha subunit, RpoA. Starting with a recombinant Escherichia coli strain encoding the lux operon and LuxR variant I206E, a random chemical mutagenesis was performed on a vector encoding RpoA. Following transformation of the mutated plasmids encoding RpoA, high throughput luminescence …
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Molecular Level Interaction of Human Fibroblast Growth Factor-1 (hFGF-1) With Phloridzin
… hFGF-1 needed for the study was expressed in recombinant Escherichia coli cells. The expressed protein was then purified using heparin sepharose affinity chromatography. Both expression and purification were monitored using SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis). …
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Experimental Methods in Support of the Development of a Computational Model for Quorum Sensing in Vibrio fischeri
… genetic constructs were transformed in a recombinant Escherichia coli strain to specifically identify genetic and biochemical elements involved in the regulation of the lux genes under diverse conditions. Then the quantitative data extracted from batch culture and microfluidic assays were …
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Analysis of the Regulons Controlled by Transcriptional Regulators LuxR and LitR in Vibrio fischeri
… were directly activated via LuxR-3-oxo-C6-HSL in recombinant Escherichia coli. The sites of transcription initiation were established via primer extension analysis. Based on the position of the lux box-binding site near position â 40, all three promoters appear to have a class II-type promoter …
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Structure/Function Analysis of the Quorum-sensing Regulator EsaR from the Plant Pathogen Pantoea stewartii
… as a weak activator of the lux operon in recombinant Escherichia coli. This thesis research further characterized the role of EsaR as an activator. Variant forms of EsaR with deletions or single residue substitutions were generated and their ability to regulate transcription was examined …
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Influência das condições de cultivo na produção de antígenos recombinantes de Leishmania i. chagasi utilizando Escherichia coli M15 cultivada em incubador rotativo e biorreator
Escherichia coli has been one of the most widely used hosts in recombinant protein production, in both laboratory and industrial scale since the advent of recombinant DNA technology. Despite the substantial progress of studies on the molecular biology and immunology of infections, there is …