Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 13 of 13 for “"Recombinant E. Coli"”.
-
Improving the recovery of "difficult to release" periplasmically-expressed products from recombinant E. Coli
The periplasm of E. coli has been recognized a suitable location for the production of large quantities of many industrially important soluble recombinant proteins, and offers some important advantages over both intracellular and extracellular production. The oxidative environment of the periplasm …
-
Ganzzellbiotransformationen mit rekombinanten Escherichia coli zur Synthese chiraler Alkohole
… addition of external cofactors. In this study recombinant Escherichia coli cells were applied as biocatalysts for the reduction of ketones. By the expression of suitable alcohol dehydrogenases (adh) both enantiomeres of chiral alcohols can be produced. The syntheses of enantiopure …
-
Microbiology of periodontal disease in Singapore and the discovery of a bacterial intron in Actinobacillus actinomycetemcomitans
… (RT-PCR) bands of 2.1 kb, 1.5 kb and 0.9 kb. Recombinant E. coli possessing the cdt gene gave similar results. In vitro transcribed cdt RNA catalyzed its own excision. The spliced products possessed different 5a?? exons but shared the same 3a?? exon with ribosome-binding site present upstream. …
-
Selection and optimization of gene targets for the metabolic engineering of E. coli
… in the metabolism of the bacterium Escherichia coli. Three case studies illustrate three disparate approaches to identifying genetic interventions: (i) combining metabolomic measurements with thermodynamic calculations to identify rate-limiting reaction steps in a target pathway; (ii) use of …
-
Functional Protein Delivery Using Polymeric Nanoparticles: A Novel Therapeutic Approach to Alpha-Synuclein Aggregation and Parkinson's Disease
… When primary neuronal cultures are treated with recombinant E. coli beta-galactosidase as nanoparticle cargo, persistent enzyme activity is measured beyond the period of nanoparticle degradation. Delivery of the small GTPase rhoG induces neurite outgrowth and differentiation in PC12 cells. In …
-
Understanding the Molecular Level Interactions of Cancer Inhibitor Imatinib with Human Fibroblast Growth Factor-1
… elusive. In this study, expression of hFGF-1 in recombinant E. coli was carried out, and the expressed protein was purified using heparin affinity column chromatography. The structural interactions governing imatinib-hFGF-1 interaction was studied by monitoring its stability, conformation and …
-
Studies of the genome and regulatory processes of Vibrio parahaemolyticus
… RIMD2210633. CsrA was first characterized in E. coli as a global regulator of carbon storage and metabolism. RIMD2210633 contains a CsrA homolog and was predicted to contain four CsrA-regulating sRNAs (CsrB1-3 and CsrC), and this work confirmed that these sRNAs regulate CsrA in the same manner as …
-
Forward and inverse metabolic engineering strategies for improving polyhydroxybyrate production
… in Synechocystis PCC6803 [IME] and recombinant E. coli [FME] in this thesis.IME was appropriate for Synechocystis, where metabolic regulation of the native PHB pathway was not well understood. A high throughput screening method was established by developing a staining protocol that …
-
Development of Oral Vaccines Against Lyme Disease
… an array of oral vaccine candidates based on recombinant E. coli that express B. burgdorferi’s OspC type K, OspB, BBK32, and Ixodes scapularis Salp15 and Salp25. Only oral immunization with live E. coli expressing OspC K induced systemic immune responses characterized by high levels of OspC …
-
Cloning, Heterologous Expression, and Characterization of Human and Escherichia Coli Choline Dehydrogenases
… in mammals and in some bacteria such as E. coli and P. aeruginosa . In mammals, it is an important source of one-carbon units for cytosolic folate- and AdoMet-dependent reactions, while in E. coli, betaine-glycine, the final product of choline oxidation, acts as an osmopretectant compatible …
-
The Influence of Changes In theMolecular Structure of N-Acylhomoserine Lactones On the Complexation and Dna-Binding of Luxr Protein
… bodies when overexpressing LuxR in Escherichia coli. The addition of its native autoinducer, N-(3-oxohexanoyl)-homoserine lactone (3OC6-HSL), during bacterial growth has made it possible to purify soluble, native LuxR from recombinant E. coli. The goal of my research was to investigate the …
-
Role of region 4 of the sigma 70 subunit of RNA polymerase in transcriptional activation of the lux operon during quorum sensing
… The specific role of region 4 of the Escherichia coli sigma 70 subunit of RNAP in LuxR-dependent transcriptional activation of the luxI promoter has been investigated. Rich in basic amino acids, this conserved portion of sigma 70 is likely to be surface-exposed and available to interact with …
-
Optimization of Fermentation Conditions for the Production of Legionaminic Acid in Recombinant Escherichia Coli
… In this work, fed-batch cultivations of recombinant Escherichia coli BRL04 were carried out in shake flasks and 5-L bioreactors. The final process was optimized by determining the effects of different carbon sources, induction temperatures, pH, dissolved oxygen (DO) content, induction …