Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"Recognition element"”.
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Expansion of predicted RiPP biosynthetic sequence space using the RiPP recognition element
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-08-01
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Development of a thermometric sensor for fructosyl valine and fructose using molecularly imprinted polymers as a recognition element
… to produce biomimetic materials with molecular recognition properties by design rather than evolution. Molecular imprinting is one way to prepare such materials. Such smart materials with new functionalities are at the forefront of the development of a relevant number of ongoing and perspective …
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DNA based biosensors for environmental and medical applications
… analytical information using a biological recognition element which is in contact with a transduction element. The first DNA biosensor realized was applied to the rapid screening of toxic substances. The biosensor was constructed immobilizing a double helix DNA (Calf Thymus DNA) onto …
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Investigations into the molecular basis of enzyme-catalyzed reactions in natural product biosynthesis
… peptides requires the action of two proteins: a recognition element, and a protease. This subset is hence referred to as split-B lasso peptides. It has been previously demonstrated that split-B lasso peptides possess a conserved motif in their leader peptides that are key to recognition element …
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Eis analysis of shear enhanced microfluidic lab-on-a-chip device
… Whenever the macromolecules interact with a bio-recognition element on the electrode transducer surface, a measurable change in the electrical current or potential takes place. To achieve lower limits of detection, the use of sensor surfaces modified with nanostructured materials such as …
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Gene Expression in Archaea: Studies of Transcriptional Promoters, Messenger RNA Processing, and Five Prime Untranslated Regions in Methanocaldococcus Jannashchii
… with a TATA box and a transcription factor B recognition element being the core promoter elements. Protein-coding gene promoters bind transcription factors less tightly than do most tRNA gene promoters. Results show a correlation between promoter sequence and transcription factor binding …
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Composite Films for Modifying Evanescent Wave Characteristics in Long-Period Grating Biosensors
… are detection devices that couple biological recognition elements to physiochemical transducers to generate quantifiable signals. Immunosensors are biosensors that use antibodies as the recognition element. The highly specific nature of antibody-antigen binding is exploited to create …
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Investigating the enzyme activity of a <i>Clostridioides difficile</i> amidase complex
… into muramic--lactam (MAL). MAL serves as a recognition element for germination-specific lytic enzymes that degrade cortex peptidoglycan layers during germination. Without the MAL residues the endospore cannot complete germination. Unique to the Peptostreptococcaceae family, which includes C. …
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Ultrasensitive Biodetection based on Plasmonically-active Materials
… of thesis, we focus on the design of molecular recognition element at the inter-face between plasmonic nanostructures and target biomolecule. We have designed specific and sensitive plasmonic biosensors based on synthetic biorecognition elements (artificial antibodies), to realize specific, …
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DNA-protein interactions throughout the Arabidopsis calmodulin-3 gene
… were used to identify putative cis-acting elements in the calmodulin-3 gene and the types of nuclear factors with which they interact with the goal of defining the molecular mechanisms regulating calmodulin-3 transcription. The $-$685 to $-$385 region of the calmodulin-3 promoter interacts …
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Elucidation and control of substrate recognition during RiPP biosynthesis
… 2, I report my investigations into the substrate recognition of canonical cyclodehydratases. Recent work suggested that unrelated RiPP modifying enzymes contain structurally similar precursor peptide binding domains. Using profile hidden Markov model comparisons, I discovered related and …
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Towards paper-based micro bio-sensing of biomarker anti-mycolic acid antibodies for TB diagnosis
… antibodies (gallibodies) as the labelled bio-recognition element. Casein hydrolysate was newly applied as a blocker in enzyme-immuno assay (EIA) to characterise the functionality of the gallibodies in order to circumvent import restrictions on bovine milk products. MA dissolved in hexane and …
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HIF-2: Standing Guard at the Crossroads of Stress and Aging
… partner ARNT/HIF-1 alpha, bind to the same recognition element located in target gene promoter or enhancer regions. However, despite these similarities, HIF-1 alpha and HIF-2 alpha regulate distinct target genes. In previous studies from the Garcia laboratory using mouse knockout studies, we …
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Characterizing peptide-modifying enzyme activity in RiPP biosynthesis
… one, the interactions between a RiPP precursor recognition element (RRE) and its cognate lasso peptide leader peptidase was deciphered by evolutionary covariance, NMR spectroscopy, and enzyme activity assays. It was shown that the RRE both delivers the leader peptide and completes the S2 pocket …
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Oligomers of beta-L-arabinosides of hydroxyproline : synthesis of the carbohydrate epitope of the Art v 1 allergen
… of hydroxyproline – was found to be a key recognition element for antibodies generated in response to the natural protein. This dissertation details the chemical synthesis of oligomers of â-arabinosides of hydroxyproline and the search to establish the minimal carbohydrate epitope of Art v …
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Characterization of SsoSSB, Sso1450, Sso2001 Proteins and Analysis of CRISPR and cas Genes from Sulfolobus solfataricus P2
… could be shown to contain Transcription Factor B recognition element (BRE), TATA-box, Shine-Dalgarno and terminator sequences specific for Sulfolobus. Most cas genes could not be expressed in a soluble form in E.coli, even when the expression conditions were widely varied. Refolding of the …
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Electrochemical Tape-and-Paper-Based Sensor for the Quantification of Potassium
… on the surface of electrodes as a potassium recognition element. Potassium ions intercalate into the Prussian blue lattice and subsequently changes the electrochemical characteristics of Prussian blue such as the redox peak potentials. These devices are highly robust, feature a limit of …
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Biomolecular NMR studies of the structure and biosynthesis of RiPP natural products
… the full backbone assignment of the FusE RiPP recognition element and utilized chemical shift perturbations to characterize the precursor peptide-FusE interactions. Solvent paramagnetic relaxation enhancements were used to characterize the interactions between FusE and the leader peptidase …