Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 29 for “"Receptor-Binding Domain"”.
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Generation and Characterization of Flavivirus Receptor-Binding Domain Mutants and Chimeras
… uncertain, but potentially involves the putative receptor-binding domain of the envelope glycoprotein, domain III (EIII). In order to determine the tolerance for heterologous sequences, and to characterize the role of EIII in virus growth and tissue tropism, chimeric viruses were generated using …
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Sub-Picomolar Detection of SARS-CoV-2 RBD via Computationally-Optimized Peptide Beacons
… detection of the SARS-CoV-2 spike protein receptor-binding domain (SRBD). The beacons contain two peptides, together forming a heterodimer, and a binding ligand between them to detect the presence of S-RBD. In the absence of S-RBD (OFF), the peptide beacons adopt a closed conformation that …
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Immune response to and pathogenesis of porcine epidemic diarrhea virus
… viral fecal shedding, and identify the putative receptor-binding domain of the virus.</p> <p>The first study characterized clinical and antibody response of pigs following experimental PEDV infection/re-infection. After experimental inoculation, naive 3-week-old pigs developed diarrhea starting 2 …
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Spike Processing and Protease Usage Effects on SARS-CoV-2 Pathogenesis
… of studies on the spike protein and its domains. While most studies focus on the impact of receptor binding domain changes, mutations in the C-terminus of S1 (CTS1) have largely been overlooked. The SARS-CoV-2 CTS1 contains various features that are unusual to sarbecoviruses – the furin …
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Phenotypic Characterization of Mutations Made to the Envelope Protein of West Nile Virus
… envelope (E) protein of flaviviruses has three domains and is the major component of the virion surface, with the EIII being the putative receptor binding domain. Previous studies showed that Gly to Ala mutation of EIII residue 331 (G331A) yielded a virus that had altered antigenicity, slightly …
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Computational Analysis of Antibody Binding Mechanisms to the Omicron RBD of SARS-CoV-2 Spike Protein: Identification of Epitopes and Hotspots for Developing Effective Therapeutic Strategies
… examine the impact of Omicron mutations on the binding affinity of four categories of antibodies that target the Omicron receptor binding domain (RBD) of the Spike protein. The study demonstrates that the Omicron variant harbors 23 unique mutations across the RBD regions I, II, III, and IV. Of …
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Nanomechanical Analysis of Coronavirus Spike proteins and Correlation with Infectivity and Lethality
… through the physical interaction between the receptor-binding domain (RBD) of its spike protein and the human cell receptor ACE2. As an increasing number of variants of SARS-CoV-2 circulates globally, estimates of infectiousness and lethality of newly emerging strains are important. Here, we …
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Efficient Biomolecular Computations Towards Applications in Drug Discovery
… is to improve the accuracy of protein-ligand binding calculations in the implicit solvent framework. To address the problem (1) GBNSR6, an accurate yet efficient flavor of GB, has been thoroughly explored in the context of protein-ligand binding, (2) a global multidimensional optimization …
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Toxin-membrane interactions of Pasteurella multocida toxin and homologous domains
… at three stages during intoxication: 1) receptor binding to the cell surface, 2) membrane translocation, and/or 3) intracellular membrane targeting. To understand the intoxication process, it is necessary to develop an understanding of the structures, dynamics, and mechanisms through …
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Computationally Designed Peptide Binder and Molecular Beacon for SARS-CoV-2
… peptide that targets the viral spike protein receptor-binding domain (RBD). The peptide was derived utilizing the existing crystal structure of spike protein’s RBD and ACE2 to determine the linear peptide fragments that contributed the most to the binding energy of the complex. We tested these …
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Receptor Binding Domains and Coronavirus Adaptation and Evolution
… is a viral membrane protein involved in host receptor binding and fusion of the viral and host cell membranes. Reported here is work performed on the spike proteins of two human common cold causing coronaviruses: HCoV-229E and HCoV-OC43. Specifically, the X-ray crystal structures of the …
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Immune Transcriptome and B cell receptor repertoire in COVID-19
… TNF and IL-6. In addition, we performed B cell receptor repertoire analysis of SARS-CoV-2 infected individuals and recipients of SARS-CoV-2 vaccine. B cells play a central role in the immune response to both SARS-CoV-2 infection and vaccination. We found marked differences in the global BCR …
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Development of monoclonal antibodies targeting T cell coinhibitory proteins TIM-3 and TIM-4
… antibodies to block checkpoint proteins from binding with their partner proteins to help overcome dysfunctional T cells and promote the recognition and elimination of tumour cells. The exploration of novel immune checkpoint targets, like T cell immunoglobulin mucin 3 and 4 (TIM-3 and TIM-4), …
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Thioaptamers Targeting Dengue Virus Type-2 Envelope Protein Domain III
… hemorrhagic disease. DENV binds the cell surface receptors and fuses with the host cell membrane using surface envelope protein (E). The E protein ectodomain contains three domains, EDI, EDII and EDIII. The highly immunogenic EDIII is the putative receptor binding domain that also binds …
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PEGylated Tissue Repair Peptide TP508 Derivatives as Second Generation Drugs for Radiomitigation and Other Systemic Applications
… a therapeutic peptide that represents a specific receptor-binding domain of human thrombin with many potential clinical applications. However, it shares several weaknesses common to peptides when administered systemically, such as a short circulating half-life and rapid proteolytic degradation. …
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Expression and Purification of the C-Terminal Domain of Porcine Epidemic Diarrhea Virus (PEDV) S1 Protein
… into the host cell. The S protein is made of two domains, the S1 domain which allows for protein interactions between the virus and the host cell, and the S2 domain which allows for membrane fusion. Because of the S1's role in protein interaction, it is often the target of potential vaccines. …
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Charakterisierung der Prototyp Foamyvirus Hüllglykoprotein Rezeptorbindungsdomäne
Spumaretroviren, oder Foamyviren (FV), unterscheiden sich von Orthoretroviren durch mehrere Besonderheiten in ihrer Replikationsstrategie. Das Partikel-assoziierte Hüllglykoprotein (Env-Protein) des „Prototype Foamy Virus“ (PFV) ist im Vergleich zu anderen retroviralen Hüllglykoproteinen …
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Structural Basis of Bat and Raccoon Dog Angiotensin-Converting Enzyme 2 (ACE2) Receptor Recognition by Sarbecoviruses
… dissertation investigates the molecular basis of receptor recognition in two species relevant to the emergence of COVID-19: the horseshoe bat (Rhinolophus sinicus) as a potential natural host and the raccoon dog (Nyctereutes procyonoides) as a suspected intermediate host. Coronaviruses evolve to …
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Engineering translational vaccine delivery systems with the polyphenol tannic acid
… eliciting durable antibody titers against the receptor-binding domain (RBD) of SARS-CoV-2 and variants of concern in mice. This system, termed TAPER (Tissue-Adhesive Polyphenol-mediated Enhanced Retention), provides many benefits including one-pot synthesis, scalability, low cost, and …
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CHARACTERIZATION OF THE INTERACTION BETWEEN HEPARAN SULFATE AND THE SPIKE (S) PROTEIN OF THE LATEST SARS-COV-2 VARIANTS
… cytokines, chemokines), and the regulation of receptors and proteolytic enzymes (e.g., tyrosine kinase-type growth factor). Moreover, HS is also involved in diverse physiological and pathological events, such as embryonic development, inflammatory response, blood coagulation, and bacterial and …
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