Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"Receptor ligand binding"”.
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Structural Studies on the Estrogen Receptor Ligand Binding Domain Using Electrospray Ionization Mass Spectrometry
A protocol using LC/ESI-MS and LC/ESI-MS/MS was developed and used successfully to identify the site covalent attachment of the photoaffinity label hexestrol diazirine. The application of tandem MS/MS sequencing permitted the identification of several residues near the C-terminus of the protein.
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In Vitro studies of factors which may influence ligand binding, function, immunogenicity and genetic regulation of the Beta-2 adrenergic receptor in asthma
… into factors which influence the expression, ligand binding and functional activity of the beta-2 adrenergic receptor. These studies were prompted by previous reports that the postulated beta-2 adrenergic receptor abnormality in allergic asthma could be induced, induced by autoantibodies. I …
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The role of nerve growth factor and p75 neurotrophin receptor in recovery from liver fibrosis
… apoptosis through p75 neurotrophin receptor ligation. Hepatic myofibroblast apoptosis is critical to resolution of liver fibrosis. I show that human hepatic myofibroblasts exhibit differential responses to mature and pro-nerve growth factor/p75 neurotrophin receptor-mediated signals. …
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A micromechanical biosensor with inherently differential readout
… application to protein detection using aptamers; receptor molecules produced in vitro. The sensor is composed of two adjacent cantilevers that form a sensor-reference pair, whereby only the sensing surface is activated with receptor molecules that are specific to the ligand to be detected. The …
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Mathematical methods for the modelling of cell migration and chemotaxis
… This scheme is used to model diffusion of ligand molecules in the region exterior to the moving cell. Simulations are presented of this new two-dimensional model indicating the effect of cell movement and receptor-ligand binding dynamics on the cell micro-environment and research is …
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Development of a State-of-the-Art Atomic Force Microscope for Improved Force Spectroscopy
… the accuracy of the AFM such that molecules and receptor-ligand binding events can be investigated with greater detail. Furthermore, the incorporation of a graphical user interface and automated data collection and analysis tools has made the AFM a more user-friendly, efficient instrument for the …
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Developing a selective sphingosine-1-phosphate-5 (s1p5) radiotracer to image oligodendrocytes using preclinical positron emission tomography (PET)
… develop novel Positron Emission Tomography (PET) ligands to specifically bind to oligodendrocytes in the central nervous system in vivo. The ligands target the sphingosine-1-phosphate-5 (S1P5) receptor as a potential marker of oligodendrocyte function with PET. The ligands selected (TEFM180 and …
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A Systems-Based Approach to Examine the Molecular Basis of Interactions between Human Monocytes and Human Micro-Vascular Endothelium
… monocyte subsets in order to identify potential receptor-ligand binding pairs. Six putative receptor-ligand pairs were taken forward for functional analysis. In order to facilitate this, a novel in vitro migration assay was developed to enable simultaneous monitoring of the locomotory behaviour …
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Transcription Factor Engineering: Tools and Applications
… useful tools in molecular biology. The estrogen receptor alpha is one of the most studied human transcription factors and acts as a ligand controlled regulator of transcription. The modular design of this and other transcription factors allows for the rational design of artificial gene switches …
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Screening of the human tumor necrosis factor (TNF) gene and its receptor 1 (TNFR1) gene for DNA alterations and the subsequent investigation of these and an IL12p40 polymorphism for an association with paediatric tuberculosis
… found to lack expression of the interferon gamma receptor ligand-binding chain (IFN-yR1) on their cell surfaces due to a single point substitution resulting in a truncated protein. Since then other defects in the type 1 cytokine pathway leading to susceptibility to non-tuberculous mycobacteria, as …
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Uncovering Zn2+ and Ca2+ Homeostasis and Crosstalk in Primary Hippocampal Neurons
… in cells exposed to pathologic stimuli or upon receptor ligand binding. In the first project of my dissertation, for the first time, we revealed that developing neurons fire spontaneous and synchronous Zn<sup>2+</sup> spikes at a specific time window (from 14 to 25 days in vitro (DIV)) without …
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Kinetic analysis of Fcγ receptor and T cell receptor interacting with respective ligands
Low affinity Fcg receptor III (FcgRIII, CD16) triggers a variety of cellular events upon binding to the Fc portion of IgG. A real-time flow cytometry method was developed to measure the affinity and kinetics of such low affinity receptor/ligand interactions, which was shown as an easily operated …