Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 95 for “"Receptor ligand"”.
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Gonadotropin releasing hormone receptor ligand interactions
… regulator of reproductive function. It binds to receptors on the gonadotrope cells of the pituitary and stimulates release of luteinizing hormone (LH) and follicle stimulating hormone (FSH). Eleven different structural forms of GnRH have now been identified in various animal species. Chimaeric …
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Structural Studies on the Estrogen Receptor Ligand Binding Domain Using Electrospray Ionization Mass Spectrometry
A protocol using LC/ESI-MS and LC/ESI-MS/MS was developed and used successfully to identify the site covalent attachment of the photoaffinity label hexestrol diazirine. The application of tandem MS/MS sequencing permitted the identification of several residues near the C-terminus of the protein.
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Quantification of receptor-ligand interactions on bioengineered autocrine call system : a comparision of theory and experiments
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 1997.
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Pancreatic Ribonuclease Functions As An Epidermal Growth Factor Receptor Ligand Independently of Its Enzyme Activity and Contributes to Cetuximab Resistance
… suspected to have an unidentified cell surface receptor. Epidermal growth factor receptor (EGFR), a well-characterized receptor tyrosine kinase is an effective therapeutic target in multiple cancer types. However, clinical trials targeting EGFR have not demonstrated improved therapeutic efficacy …
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The G-protein coupled receptor CMKLR1/ChemR23: Studies on gene regulation, receptor ligand activation, and HIV/SIV co-receptor function
… intercellular communication. G-protein coupled receptors (GPCRs), located on the cell surface, play an important role in this communication. Cells synthesize and release signalling molecules (ligands), which produce a specific response only in those cells that have a receptor for that ligand. …
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Computational analysis of cell-cell communication in the tumor microenvironment
… therapeutic resistance. As a result, targeting receptor-ligand interactions, for instance with immune check-point inhibitors, can provide significant benefit for patients. However, our knowledge of this complex network of cell-cell interactions in a tumor microenvironment is still incomplete, …
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Genetic Investigation of MHC-independent Missing-self Recognition by NK Cells
… and more specifically, the NKR-P1B:Clr-b receptor-ligand system and its role in bone marrow transplantation. In the first results chapter, we generated hybridoma clones producing monoclonal antibodies against another inhibitory receptor in the Nkrp1 receptor family, NKR-P1G via the CELLISA …
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The role of nerve growth factor and p75 neurotrophin receptor in recovery from liver fibrosis
… apoptosis through p75 neurotrophin receptor ligation. Hepatic myofibroblast apoptosis is critical to resolution of liver fibrosis. I show that human hepatic myofibroblasts exhibit differential responses to mature and pro-nerve growth factor/p75 neurotrophin receptor-mediated signals. …
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Experimental and computational studies of receptor-mediated cell detachment in shear flow
Specific, receptor-mediated adhesion of cells to ligand-coated surfaces in viscous shear flow is a central phenomenon in many physiological and biotechnological processes. Mechanical strength of specific adhesion is generally presumed to be related to chemical affinity of receptor/ligand bonds, but …
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Engineering Antibodies to Control T Cell Function
… display system was used to show that a T cell receptor ligand (scFv-KJ16) expressed on the surface of yeast was capable of inducing T cell activation. These findings suggest that the yeast display system can be used to discover novel polypeptides that regulate biological functions through cell …
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A Systems-Based Approach to Examine the Molecular Basis of Interactions between Human Monocytes and Human Micro-Vascular Endothelium
… monocyte subsets in order to identify potential receptor-ligand binding pairs. Six putative receptor-ligand pairs were taken forward for functional analysis. In order to facilitate this, a novel in vitro migration assay was developed to enable simultaneous monitoring of the locomotory behaviour …
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Extracellular Interaction Screening Using CRISPR Activation
Extracellular interactions between cell surface receptors are necessary for signaling and adhesion but identifying them remains technically challenging. We describe a cell-based genome-wide approach employing CRISPR activation to identify receptors for a defined ligand. We show receptors for …
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Engineering Estrogen Receptor-Based Gene Switches and a Superoxide Dismutase for Therapeutic Applications
… used to engineer variants of the human estrogen receptor alpha (hERalpha) that bind and respond to two synthetic small molecule ligands---4,4'-dihydroxybenzil (DHB) and 2,4-di(4-hydroxyphenyl)-5ethylthiazole (L9)---with high specificity and potency. The resulting highly specific DHB/7-S receptor …
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Molecular insights into signalling mechanisms within TGF-β superfamily
The TGF-β superfamily includes receptors, extracellular signalling molecules, their inhibitors and intracellular downstream signalling components. Members of the superfamily are present in all metazoans, expressed in all cells of the body and they are involved in embryonic development, adult tissue …
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In Vitro studies of factors which may influence ligand binding, function, immunogenicity and genetic regulation of the Beta-2 adrenergic receptor in asthma
… into factors which influence the expression, ligand binding and functional activity of the beta-2 adrenergic receptor. These studies were prompted by previous reports that the postulated beta-2 adrenergic receptor abnormality in allergic asthma could be induced, induced by autoantibodies. I …
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Antibody-Antigen assay design for combined optical tweezers and fluorescence
… promises to enable unbindinig force studies of receptor-ligand interactions, whose specificity play a crucial role in the function of many biological systems. This thesis focuses on the development of assay designs for the study of antibody-antigen binding interactions using combined optical …
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Biophysical regulation of cell motility by adhesion ligands and growth factors : effect of spatial presentation of the ligand
… mammalian cell migration based on principles of receptor/ligand interactions involved in its regulation. Recent studies have shown that integrin cell surface receptors for the extracellular matrix (ECM) proteins initiate signaling cascades, some of which are in common with those initiated by …
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The Design of Polyelectrolyte Multilayers Using Galactosylated Chitosan
… a surface protein, called the asialoglycoprotein receptor (ASGP-R), which interacts with galactose via a specific receptor-ligand interaction. Polyelectrolyte multilayers (PEMs) were prepared by the layer by layer method, which is based on electrostatic attractions between oppositely charged …
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Amino Acid Residues Implicated in the Interaction of Melanocortin Ligands and Their Receptors: A Study of MC2R Selectivity
<p>Melanocortin receptor ligand selectivity has been a question not easily answered. The inability to functionally express melanocortin 2 receptor (MC2R) has inhibited the study of why MC2R is only stimulated by ACTH, a melanocortin hormone. With the recent discovery of the MC2R accessory protein …
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Therapeutic applications of DNA origami-based programmable nanoparticles
… platforms only offer control over average ligand density. In this thesis, we demonstrate the therapeutic utility of DNA origami for cancer and infectious diseases. First, we demonstrate that modulating the nanoscale arrangement of an adjuvant enhances the efficacy of cancer vaccines. …
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