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Showing 1 to 13 of 13 for “"RecQ"”.
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The functions of RECQ protein-like 4 and high mobility group box 1 in osteoblast-like cells
This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).
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Single molecule studies of helicase mechanisms
Bloom helicase (BLM) is a member of the RecQ helicase family, a group of 3’ to 5’ helicases which are homologs of the RecQ protein found in E. coli. Besides the canonical helicase function of unwinding duplex DNA, the RecQ family of helicases is known to preferentially bind to non-canonical DNA …
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Effect of Werner and Bloom Helicase Deficiencies On Dna Double-Strand Break Repair
… or collapse of stalled replication forks. The RecQ helicases are a family of helicases known to aid in the repair of these DSBs and help maintain genomic stability and integrity. WRN and BLM are helicases in the RecQ helicase family. Mutations in the genes for each helicase result in a severe …
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Flap Endonuclease 1 ensures telomere replication and stability
… activity of FEN1, its interaction with the RecQ helicases and its ability to process DNA bubble structures are essential to prevent telomere loss, whereas its ability to process Okazaki fragments is dispensable. However, FEN1 depletion did not affect cell cycle progression or in vitro DNA …
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THE FUNCTION OF MRN (MRE11-RAD50-NBS1) COMPLEX DURING WRN (WERNER) FACILITATED ATM (ATAXIA-TELANGIECTASIA MUTATED) ACTIVATION
WRN (Werner) protein is a member of the RecQ family showing helicase and exonuclease activity. WRN protein may lose function upon mutation and causes Werner syndrome (WS) which is an autosomal recessive, cancer-prone and premature aging disease. ATM (Ataxia-Telangiectasia mutated) protein initiates …
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The Biochemical Characterization of Drosophila melanogaster RecQ4 Helicase
<p>RecQ4, a member of the conserved RecQ family of helicases, is involved in replication and associated with several clinical syndromes. Although biologically important, the biochemistry of RecQ4 has remained elusive. We have expressed and purified Drosophila melanogaster RecQ4 from a baculovirus …
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Characterization and consequences of Telomere replication gone awry
… and the fission yeast member of the conserved RecQ helicase family, Rqh1. A novel sumoylation deficient allele of Rqh1 was able to suppress several taz1Δ-specific phenomena without dramatically affecting other genome-wide functions of Rqh1. Interestingly, genetic analysis revealed that this …
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Investigating the regulation of DNA non-homologous end-joining through Ku70/80 interacting factors
… system. Lastly, I investigate the role of the RecQ helicase WRN, whose precise roles in the DDR are unclear. As an interactor of both HR and NHEJ proteins, WRN may affect the regulation of both pathways. WRN knockout cells were generated and a CRISPR-Cas9 screen performed to identify …
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Ethical Climate, Organizational Commitment, and Job Satisfaction of Full-Time Faculty Members
… 2) Revised Ethical Climate Questionnaire (RECQ) created by Victor and Cullen (1993), and 3) Job Diagnostic Survey (JDS) created by Hackman and Oldham (1980).</p><p>The data analysis found significant differences in self-reported levels of organizational commitment and job satisfaction for …
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The Function and Mechanism of RNA Interference in Neurospora
… by Replication Protein A (RPA) and QDE-3 (an RecQ helicase), both of them are also essential for aRNA production. Moreover, QDE-1 can produce dsRNA from ssDNA, a process facilitated by RPA. Our results provide a molecular mechanism of aRNA production in RNAi pathway.
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The Mechanism of RNA Interference in Neurospora
… RNA dependent RNA polymerase, RdRp) and QDE-3 (a RecQ helicase) functions in quelling pathway by generating double-stranded RNA (dsRNA) from transgenes. To further understand the importance of QDE-1 and QDE-3, quelling assays were performed in the qde-1ko and qde-3ko strains. In contrast to …
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PARKIN, BLM AND EXO1: INVOLVEMENT IN POST-NER ACTIVITY IN NON-REPLICATING CELLS
… the possible involvement of the Bloom syndrome RECQ-like helicase (BLM) in the response to COLs, since it is known to work in conjunction with EXO1 helicase in other processes like homologous recombination. Our data show that BLM is recruited to sites of local UV damage with kinetics similar to …
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Somatic microsatellite variability as a measure of DNA stability in cancer and DNA repair disorders
… Werner syndrome, caused by the loss of the RecQL2 gene; and Rothmond-Thomson syndrome, caused by the loss of the RecQL4 gene. The goal of this project was to determine if impaired excision repair genes CSA or global XPA and B or excision repair supporting helicases BLM or RecQL4 leads to MST …