Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"RecA Filament"”.
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Single molecule fret study on the mechanism of RecA mediated strand exchange
RecA plays a critical role during double strand break repair via homologous recombination. During the strand exchange reaction, RecA forms a helical filament on single stranded (ss) DNA that searches for homology and exchanges complementary base pairs with a homologous double strand (ds) DNA to …
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Single-molecule FRET study on the RecA-mediated DNA repair
The RecA protein helps maintain genomic integrity through recombination. Using single-molecule assays and hidden Markov modeling, we show the most direct evidence that a RecA filament grows and shrinks primarily, one monomer at a time only from the extremities. Both ends grow and shrink, contrary …
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Generation of SOS inhibitors as co-drugs to potentiate the activity of bactericidal antibiotics and to block the emergence of antibiotic resistance
… SOS response by promoting the formation of the RecA filament, which in turn activates the auto-cleavage activity of LexA and allows expression of SOS genes, including the SOS error-prone polymerases. In this project, phthalocyanine tetrasulfonic acid (PcTs)-based RecA inhibitors were …
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Fluorescence imaging of single molecule dynamics on long single stranded DNA
… homology search on Escherichia coli (E. coli) RecA filaments formed on ssDNA, (2) the unidirectional motion of E. coli UvrD helicase and (3) the diffusion of E. coli single-stranded DNA binding protein (SSB). We observed that the homology search of dsDNA on RecA filament is comprised of pauses …
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Exploring Novel Techniques for the Single Molecule Toolkit: Vesicle Encapsulation and Immobilization
… to perform unique measurements for observing RecA filament formation, hairpin ribozyme cleavage and Rep helicase translocation within confined volumes. Novel features were revealed by such experiments unveiling new biological findings. We also discuss ideas to introduce pores that can be …
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Interplay between an integrative and conjugative element and its bacterial host
… host DNA damage repair response by preventing RecA filament formation. This activity confers a fitness advantage to the host in that it dampens the SOS response and protects against phage activation while also stabilizing ICEBs1 DNA. I also present preliminary evidence that ICEBs1 utilizes a …
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Single molecule investigations into DNA replication, recombination and repair
… repair. Srs2 dismantles presynaptic Rad51 filaments and prevents its re-formation as an anti-recombinase. However, the molecular mechanism by which Srs2 accomplishes these tasks remains unclear. Here we report a single-molecule fluorescence study of the dynamics of Rad51 filament formation …
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Binding configurations of single-stranded DNA binding protein and their influence on DNA recombinase
… while SSBs in the (SSB)35 binding mode form long filaments (unlimited cooperativity). These different levels of binding cooperativity have been proposed to be used selectively in different DNA metabolic processes, including DNA replication, recombination, and repair. In homologous recombination, …