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Showing 1 to 20 of 64 for “"RecA"”.
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The molecular bases of tryptophan replacement effects in RecA: A biochemical and biophysical characterization of single- and null- tryptophan mutant Escherichia coli RecA proteins
… are highly conserved. The Escherichia coli RecA protein is studied as a prototypic strand exchange protein with structural and biochemical similarity to the archaeal RadA and eukaryotic Rad51 family of recombination proteins. Although the RecA protein has been extensively investigated, …
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Single-molecule FRET study on the RecA-mediated DNA repair
The RecA protein helps maintain genomic integrity through recombination. Using single-molecule assays and hidden Markov modeling, we show the most direct evidence that a RecA filament grows and shrinks primarily, one monomer at a time only from the extremities. Both ends grow and shrink, contrary …
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Subcellular localization and visualization of RecA and ImuAʹ in mycobacteria
… and mutagenesis in mycobacteria is required. RecA and ImuAʹ are DNA damageinducible proteins implicated in DNA damage repair and tolerance in Mtb. RecA is a key regulatory protein of the SOS response and ImuAʹ is a component of the mycobacterial mutasome, effecting DNA damage tolerance and …
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Single molecule fret study on the mechanism of RecA mediated strand exchange
RecA plays a critical role during double strand break repair via homologous recombination. During the strand exchange reaction, RecA forms a helical filament on single stranded (ss) DNA that searches for homology and exchanges complementary base pairs with a homologous double strand (ds) DNA to …
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The molecular characterisation of the recA locus in the opportunistic pathogen Bacteroides fragilis
… drug, metronidazole, a DNA damaging agent. The RecA protein is thought to be involved in the repair of metronidazole damage as well as damage caused by oxidative stress. The ability to survive oxygen stress is a strong indicator in an anaerobic bacterium of pathogenic potential and bacterial …
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Genetic duplication in Salmonella typhimurium
… and a PolA⁻ strain and compared to that of a RecA⁺, PolA⁺ strain. The frequency of formation was found to be reduced in both RecA⁻ and PolA⁻. strains. Loss of the duplication was eliminated in the RecA⁻ strain, while the frequency of loss was found to be greater in a PolA⁻ strain than in the …
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A genetic and biochemical analysis of LexA repressor cleavage in Escherichia coli K-12.
… by a specific cleavage reaction which requires RecA protein. Under physiological conditions in vitro, RecA-dependent cleavage also occurs. At alkaline pH, however, the specific cleavage reaction occurs spontaneously without RecA, a reaction which is termed autodigestion. The LexA repressor is, …
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Developmental genetic studies on Thiobacillus ferrooxidans
… vector, pEcoR251, for the purpose of cloning a recA-like gene from this organism. The library consisted of approximately 1,78 X 10⁴ clones carrying chromosomal DNA fragments of about 3-12 kilobases (kb). The library was successfully screened for functional complementation of E. coli auxotrophic …
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An Exploration of the Feasibility of Combining Radiation Therapy with Psoralen Phototherapy
… Enhanced with Cherenkov photo-Activation (RECA) is another approach, where therapeutic megavoltage (MV) x-ray generates UV light via Cherenkov phenomenon. Both approaches could increase local control in RT, increase treatment effectiveness in hypoxic tumors, and amplify anti-cancer systemic …
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The Role of Bacteroides Conjugative Transposons in the Spread of Antibiotic Resistance Genes, And, The Role of recA in DNA Damage Repair in Deincoccus Radiodurans
… published studies had suggested that the RecA protein, which is required for repair of DNA damage, from D. radiodurans is unique among RecA proteins and that this uniqueness accounts for this organism's ability to repair massive amounts of damage to its genome. The work presented in this …
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W-reactivation (Inducible "SOS" DNA Repair) of double stranded DNA bacteriophage ? and single stranded DNA bacteriophage fd on isogenic rec and uvrB mutants of Escherichia coli K-12
The recA mutant has been shown to be completely recombination deficient and highly UV-sensitive. Also, this mutant is remarkably deficient in inducible "SOS" DNA repair and, consequently it is not UV-mutable and it cannot perform W-reactivation, an inducible non-excision repair dependent …
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Binding configurations of single-stranded DNA binding protein and their influence on DNA recombinase
… repair. In homologous recombination, recombinase RecA must bind and form nucleoprotein filaments on the ssDNA, in direct competition with SSB. Prior studies have shown that RecA is capable of forming filaments on ssDNA wrapped by SSBs in the (SSB)65 binding mode, but filament formation on ssDNA …
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Homologous Strand Exchange and DNA Helicase Activities in Plant Mitochondria
… homologous recombination is dependent on the RecA protein. Higher plant chloroplasts have RecA-like strand exchange activity. However, little is known about these mechanisms in higher plant mitochondria. I have detected a RecA-like strand exchange activity in soybean mitochondria. This …
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Generation of SOS inhibitors as co-drugs to potentiate the activity of bactericidal antibiotics and to block the emergence of antibiotic resistance
… SOS response by promoting the formation of the RecA filament, which in turn activates the auto-cleavage activity of LexA and allows expression of SOS genes, including the SOS error-prone polymerases. In this project, phthalocyanine tetrasulfonic acid (PcTs)-based RecA inhibitors were …
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Konstruktion und Charakterisierung von Lebendvakzine-Kandidaten auf der Basis von siaD-Deletionsmutanten von Neisseria meningitidis Serogruppe B
… das für die homologe Rekombination essentielle recA-Gen deletiert werden (Koomey und Falkow, 1987; Miller und Kokjohn, 1990). Der Nachweis einer recA-Deletion gelang trotz mehrfacher Ansätze mit der neuen Transformationsmethode nicht, da die Deletionsrate von recA wahrscheinlich unter der …
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The use of alkaline gel electrophoresis to analyze hydrogen peroxide-caused DNA damage and repair in Escherichia coli
… in one or more DNA repair enzymes, viz., recA (recA), exonuclease III (xthA), DNA polymerase I (po/A) or DNA polymerase I plus exonuclease I] (polA-xthA). For DNA analysis of single-strand breaks, samples from a cell suspension were removed at 2 min intervals following an initial 15 min …
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Strategies to potentiate bactericidal antimicrobial activity based on the suppression of bacterial stress response systems
… of the SOS response (by suppressing the recA gene) and overproduction of ROS (by suppressing detoxification systems or stress regulatory systems) in increasing the activity and lethality of fluoroquinolones; (ii) to evaluate the interplay between the SOS response (DNA repair and …
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Construction and Use of a System for Studying the Integration of Bacteroides Conjugative Transposons
… recombination. For this reason, a Bacteroides recA strain was constructed and the RecA independence of conjugative transposition was demonstrated. The minielement system described here will serve as a prototype for additional studies seeking to determine the integration mechanism of the …
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DECIPHERING THE ORAL MICROBIOTA IN FOOD ALLERGY: INSIGHTS INTO THE CANDIDATE PHYLA RADIATION AND NOVEL METHODOLOGICAL APPROACHES
… alternative genetic markers, highlighting RecA as a strong candidate. Building on this, we explored its potential for bacterial community profiling, developing a machine learning algorithm that accurately infers taxonomy from RecA sequences across bacterial diversity. This algorithm was …
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