Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 62 for “"Reaction Monitoring"”.
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Birefringent single-arm fiber optic enthalpimeter for catalytic reaction monitoring
… are almost universally associated with chemical reactions. Thermometry as an analytical tool has been extensively researched and developed. Finding solutions to problems involving thermal isolation, specificity, sensitivity, and cross-sensitivity remain as active areas of interest. Fiberoptic …
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Analysis of urinary lipid biomarker candidates from tuberculosis patients by multiple reaction monitoring
… of biomarker candidates, and targeted multiple reaction monitoring (MRM) is the method of choice for subsequent validation of biomarker candidates. A list of candidate urinary biomarkers has previously been generated which can discriminate between latent and active TB infection using MS1 mass …
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Part One: A Flow Reactor With In-Line Analytics: Design and Implementation Part Two: In-Line Derivatization of Protic Compounds for GC/MS Reaction Monitoring
The creation of an automated synthetic reaction system with in-line analytics is presented in two parts. First, the design and implementation of a flow reactor system complete with in-line gas chromatography mass spectrometry (GC/MS) analytical instrument is discussed. The testing and validation of …
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Photochemical transformations of α-diazocarbonyl compounds in flow
… diazo transfer and subsequent photochemical reactions, enabling three steps to be carried out without the need to isolate or handle any hazardous materials. The use of Process Analytical Technologies (FlowNMR and FlowIR™) throughout this work provided key insights into reaction progress and …
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Novel urinary and serological markers of prostate cancer using proteomics techniques: an important tool for early cancer diagnosis and treatment monitoring
… biomarkers for early diagnosis and treatment monitoring of prostate cancer in a South African cohort as well as elucidate proteome differences in patients from our heterogeneous cohort. We compared the urinary proteomes of PCa, Benign Prostatic Hyperplasia (BPH), disease controls comprising …
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Global and targeted proteomics in Arabidopsis thaliana: A study of secondary metabolism and phytohormone signaling
… derivitization, and those that do not. Selected-reaction monitoring (SRM) for targeted proteomics, and data-independent acquisition (MSE) was also evaluated. In addition to assessing the capabilities of these technologies, we then applied them to the context of uncovering new insights into the …
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Design and implementation of an automated reconfigurable modular flow chemistry synthesis platform
… expert chemists' productivity. Automated reaction platforms, however, have been limited in their ability to access a diverse set of process units, beyond simple mixing and stirring. A system capable of carrying out multi-step syntheses, inline reaction monitoring, multi-phase reactions, …
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DEVELOPMENT OF A LC/MS/MS ENZYME METHOD FOR N8 - ACETYLSPERMIDINE MEASUREMENTS IN ENZYME ASSAYS
… the peak area of the fragment-ion SRM (selected reaction monitoring: <em>m/z</em>: 188/114) chromatograms from N<sup>8</sup> -acetylspermindine and x is the concentration of N<sup>8</sup> - acetylspermindine. Acetylation of spermidine (SPD) and N<sup>8</sup>-acetylspermidine (N8AcSPD) with …
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Enantioselective synthesis of pretomanid & investigations of Raman spectroscopy for through-tube monitoring of reactions
… of Raman Spectroscopy for Through-Tube Monitoring of Reactions [color illustrations] Continuous-flow chemistry has seen expansive growth and thus requires powerful new analytical techniques. The current commonly-used analytics involve inline analysis at a single point, which can hinder …
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Sensing Interfacial Non-Faradaic and Faradaic Processes via Plasmonic-Enhanced Metallic Luminescence in Nano-Optoelectrodes
… in bioimaging, nano-thermometry, and chemical reaction monitoring applications. Despite increasing interest in nanoplasmonic metal luminescence, little attention has been paid to investigating its dependence on voltage modulation. Also, the hyphenated electrochemical surface-enhanced Raman …
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Carbon-trifluoromethyl bond forming reactions and palladium-catalyzed cyanation of (hetero)aryl halides
… acids is reported. Bench top setup and visual reaction monitoring makes this process particularly well suited to medicinal and academic chemists. Fast reaction times allow for the trifluoromethylation of heterocyclic boronic acids that are prone to facile protodeboronation. Chapter 3 A …
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LC-MS/MS determination of various drugs of abuse and metabolites in municipal wastewater effluent samples
… ionization was used along with dynamic multiple reaction monitoring (MRM) tandem mass spectrometry for identification and quantitation. The extraction method was validated with matrix matched spiked samples at the limits of quantitation (0.5 ng/mL to 30 ng/mL) for each analyte with recoveries …
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Comprehensive proteomic profiling of clinically relevant strains of Mycobacterium tuberculosis
… proteins to use in the design of multiple reaction monitoring (MRM) MS experiments for targeted biomarker validation in patient derived biological samples such as sputum. Liquid chromatography mass spectrometry (LC MS/MS) and data capture were carried out using the LTQ Orbitrap Velos. 1D LC …
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Quantitative Approaches for Protein Differential Expression Analysis
… validation by targeted screening, i.e., multiple reaction monitoring (MRM). Preliminary experiments were performed to evaluate the performance of the spectral counting method. The method proved to be applicable for proteins with spectral counts≥2, and a close-to-linear relationship between protein …
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Part A The application of fluorescence resonance energy transfer to reaction discovery Part B The stereodefined synthesis of tetrasubstituted olefins
… development of methodology for enantioselective reaction monitoring using Fluorescence Resonance Energy Transfer (FRET) is described. The syntheses of enantiomeric epoxides bearing different fluorescent donors and a phenol that bore fluorescence acceptor were explored.* Part B describes the …
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Liquid Chromatography – Mass Spectrometry for Protein Biomarker Discovery in Pancreatic Adenocarcinoma
… to enhance the utility of the method. Multiple reaction monitoring (MRM)/MS provided sensitivity and specificity to the method and stable isotope dilution (SID) provided correction for losses during sample analysis, enabling precise quantitation and efficient determination of the concentration …
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Development of liquid chromatography with tandem mass spectrometry method for measurement of sepsis biomarkers
… Two different tandem MS modes: multiple reaction monitoring (MRM) and parallel reaction monitoring (PRM) were developed using triple quadrupole (QqQ) and Orbitrap mass spectrometer, respectively, for measurement of fragment ions of the chosen surrogate peptides. The detection performance …
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The powerful combination of novel organosulfur methodology and continuous flow technology
… their subsequent use in a Stille cross-coupling reaction to form chalcone derivatives. Key mechanistic insight into the formation of the α-sulfenyl-β-chloroenones was provided through use of reaction monitoring via a combined FlowNMR and FlowIR study. This technology expanded our understanding of …
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Computational Tools to Expand the Coverage of Metabolite Profiling via LC-MS/MS
… a foundation for designing large scale multiple reaction monitoring experiments.</p><p>With these two additions to the arsenal of LC-MS/MS based metabolite profiling tools, we move closer to one day reaching the goal of global metabolite profiling. In turn, this may lead to exciting discoveries …
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