Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 32 for “"RdRp"”.
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Norovirus Translation and Replication
… in norovirus pathogenesis, and VP1-RdRp interaction in regulating viral genome replication. MNV infection results in the phosphorylation of the translation initiation factor eIF4E, re-programming host-cell translation during infection. Inhibiting eIF4E phosphorylation reduces MNV …
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Structures, Functions, and Druggability of the SARS-CoV-2 Replication–Transcription Complex
… is mediated by the RNA-dependent RNA polymerase (RdRp) which requires a coterie of viral nucleic acid-metabolizing enzymes to maintain the integrity of the genome. The RdRp and other viral replication enzymes are postulated to interact as part of a large assembly known as the …
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Characterization of a Low-Fidelity Live-Attenuated Vaccine for Venezuelan Equine Encephalitis Virus
… mutations in the RNA-dependent RNA-polymerase (RdRp) were tested in the live-attenuated Venezuelan equine encephalitis virus (VEEV) vaccine strain, TC-83. Next generation sequencing after passage in the presence of mutagens revealed a mutant containing 3 mutations in the RdRp, TC-83 3x, to have …
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RNA replication by poliovirus RNA-dependent RNA polymerase: effects of residue 5 on elongation complex stability and processivity
The poliovirus (PV) RNA-dependent RNA polymerase (RdRP) is a small, single-subunit, enzyme that is responsible for the replication of the viral genome. PV genome replication is much more involved than just repetitively incorporating a single nucleotide into an oligonucleotide primer. The polymerase …
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Modelado matemático avanzado de procesos de polimerización por adición-fragmentación reversible
… radicalarias por desactivación reversible (RDRP) constituyen una alternativa atractiva para su síntesis, ya que permiten obtener esta clase de polímeros en condiciones operativas y de pureza de reactivos compatibles con la práctica industrial. Existe una compleja interrelación entre las …
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Identification and Characterization of the Mechanisms of Action of Novel Antiviral Compounds Targeting the Minimal Replication-Transcription Complex of SARS-CoV-2
… development, the RNA polymerase RNA dependent (RdRp) is the pharmacological target of two of the three drugs currently in clinical use for the treatment of COVID-19, namely remdesivir and molnupiravir. However, the absence of strong evidence of in vivo efficacy and safety still calls for the …
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CHARACTERIZATION OF INFLUENZA NUCLEOPROTEIN BODY DOMAIN AS ANTIVIRAL TARGET
… bound by the viral RNA dependent RNA polymerase (RdRP) to form viral ribonucleoproteins (vRNPs) responsible for RNA synthesis. NP is a structural component of the vRNP but also interacts with both viral and host factors to regulate viral RNA expression. NP is conserved among influenza A isolates, …
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The Function and Mechanism of RNA Interference in Neurospora
… into dsRNA by RNA dependent RNA polymerase (RdRP). How aRNA is produced and specifically recognized by RdRP is not known. We show that QDE-1, a RdRP is also the DNA-dependent RNA polymerase (DdRP) that produces aRNA from ssDNA. QDE-1 is recruited to ssDNA by Replication Protein A (RPA) and …
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Using Altered Fidelity Viruses to Study Viral Attenuation and Population Genetics During Vertebrate Host Infection
… viruses contain an RNA dependent RNA polymerase (RdRp) that creates a population of closely related but slightly different variants following each replication cycle. These variants can interact with each other, allowing the viral population to overcome selection pressures without adapting the …
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Stereochemical Effect of RAFT Agents on Kinetics of Reversible Deactivation Radical Polymerization
The propagation polymer chains in RDRP can be isolated in the dormant form and be re-activated at chain ends to generate propagating radicals, which initiate subsequent chain extensions. Since the effect of remote substituents on kinetics decreases rapidly with their distance from the reaction site …
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Overcoming Challenges in Reversible Addition–Fragmentation Chain Transfer Polymerization using Photoinduced Electron/Energy Transfer Catalysis
… reversible-deactivation radical polymerization (RDRP), or otherwise, single units could undergo multiple incorporations. Using expansive condition screening with PET-RAFT polymerization, a set of conditions was identified that resulted in a single-unit monomer insertion (SUMI) within the polymer …
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Using proxy markers from routine diagnostic PCR testing to assess the disease severity of new SARS-CoV-2 variants
… proxy marker, RNA-dependent RNA polymerase (RdRp) target delay in the Seegene AllplexTM 2019-nCoV polymerase chain reaction assay, to identify suspected Delta variant infection in routine laboratory data. All cases diagnosed on this assay in the public sector in the Western Cape, South …
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Controlled Radical Polymerization: Towards Industrially Relevant Conditions
Reversible deactivation radical polymerization (RDRP), also known as controlled radical polymerization (CRP), has been a significant area of polymer research for more than 20 years, allowing the facile synthesis of complex macromolecules previously unattainable by conventional free radical …
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Biochemical mechanisms for selectivity governing RNA and DNA replication
… characterize the RNA dependent RNA polymerase (RdRp) from SARS-CoV-2, a prime target for antiviral drugs to stop replication of the virus. I developed methods to express the tag free complex in E. coli and subsequent purification to give highly active enzyme, which for the first time met …
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ENDOGENOUS ANTIVIRAL PROPERTIES OF RED BLOOD CELL-DERIVED EXTRACELLULAR VESICLES AND THEIR AUGMENTATION OF ANTIVIRAL OLIGONUCLEOTIDE THERAPY
… conserved viral genes (helicase, 3CLPro, PLpro, RdRp, and TRS), delivered via RBCEVs, significantly suppressed viral replication in vitro and in vivo, improving survival in infected K18-ACE2 mice. Using the IDentif.AI platform, we identified synergistic ASO-loaded RBCEV combinations with enhanced …
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Visible Light-Mediated Polymerization for Protein-Polymer Conjugation: A Biocompatible Tool for Therapeutic Applications
… reversible-deactivation radical polymerization, RDRP) has been emerging since 1990’s to address these issues and refine the process. Notably, the recent advancements in photochemistry, such as photo-RDRP or photo-CLRP, has revolutionized the protein-polymer conjugation process. These developments …
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Strukturní a funkční charakterizace inhibice flavivirové methyltransferázy.
… s funkcí RNA- dependentní RNA-polymerasy (RdRp) a N-koncovou doménou s funkcí methyltransferasy (MTasa). Flavivirové MTasy katalyzují poslední krok v tvorbě čepičky genomové RNA, která hraje klíčovou roli v procesech virové translace, replikace a úniku před imunitním systémem hostitele. …
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Reprogramming the expression of the double-stranded RNA mitovirus OnuMV1c from the mitochondria to the cytoplasm in the fungal pathogen Ophiostoma novo-ulmi
… that encodes an RNA-dependent RNA polymerase (RdRp) involved in its replication as a double-stranded RNA molecule. It exists in O. novo-ulmi mitochondria in both its single-stranded and double-stranded forms. Our research group identified OnuMV1c as a potential candidate for biological control …
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Untersuchungen zu Struktur-Funktionsbeziehungen in der tRNA-ähnlichen Struktur des Rüben-Gelbmosaik-Virus
… mit der viralen RNA-abhängigen RNA-Polymerase (RdRp; Teil der viralen Replikase) durchgeführt, in die 85 Nukleotide-lange TLS-Varianten mit unterschiedlichen Valylierungseigenschaften eingesetzt worden waren. Die TLS-Varianten waren außerdem in Anticodon-Schleife und L1-Schleife verschieden und …
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Role of basic and hydrophobic residues in the poliovirus polymerase elongation complex and the structure of a coxsackievirus polymerase elongation complex
… require a viral RNA-dependent RNA polymerase (RdRP) for genome replication. This enzyme synthesizes negative-sense RNA from the infecting positive sense genome producing a replicative intermediate. The negative sense RNA then serves as a template for synthesis of additional positive-sense RNA. …
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