Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"Ras/MAPK"”.
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Aberrant Ras/MAPK signaling in skeletal development
The Mitogen-activated protein kinase(MAPK) signaling pathway has been studied intensively in the context of neoplastic transformation. Other studies have focused on the roles of this pathway during development and have modeled syndromes, such as Noonan Syndrome (NS), that are caused by aberrant …
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Die transkriptionelle Regulation des roughest Gens in Drosophila melanogaster
… minded (Sim), Dmef2 und nukleare Effektoren der Ras-MAPK und Notch Signalwege aktiviert werden. Innerhalb dieser aktivierbaren rst cis-regulatorischen Sequenzen befinden sich Enhancer-Module, die eine mit der bekannten Wirkung der einzelnen Faktoren korrelierbare Reportergenexpression vermitteln. …
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The rhinoceros gene of drosophila restricts cell fate specification in the developing eye
… fate determination in the developing eye, RTK/Ras/MAPK is among the most important. One effect of RTK/Ras/MAPK signaling is to downregulate the transcription factor Yan, thus allowing differentiation. A previous genetic screen identified modifiers of YanACT, a constitutively active isoform of …
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Comprehensive Analysis of the Molecular Underpinnings of Pediatric Glioma
… frequently harbor genetic alterations in the RAS/MAPK pathway, although a comprehensive molecular landscape of the disease has yet to be completed. To address this, we characterized a population-based cohort of >1,000 pLGG. We discovered that RAS/MAPK pathway dysregulation was universal in …
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The Kras/Mapk Pathway and Ligand Independent Activation of Erα: Implications For The Treatment of Endometrial Cancer
… resistance. Member kinases of the PI3K/AKT and Ras/MAPK pathways activate estrogen receptor α (ERα) independent of estrogen, however, few studies have evaluated the role of the Ras/MAPK pathway in predicting response to hormonal therapy in EC. The aims of this project were to evaluate the role …
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GENETIC CONTROL OF EYE AND CENTRAL NERVOUS SYSTEM DEVELOPMENT
… we show that homozygous deletion of Nf1, the Ras GTPase gene underlying human neurofibromatosis type 1 syndrome, caused lens dysgenesis in mouse. While early lens specification proceeded normally in Nf1 mutants, lens induction was disrupted due to deficient cell proliferation. Further analysis …
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Functional study of the IFITM1 gene related to tumor progression of NF1
… 12 6. Western blot analysis 13 7. Ras activation assay 15 8. Cell death assay 15 9. Transfection 15 10. Confocal microscopy 16 Ⅲ. 결과 17 A. 일차배양세포에서의 IFITM1의 발현양상 17 B. 악성세포에서 down-regulation 된IFITM1의 과발현(over-expression) 19 C. IFITM1 과발현에 의한 RAS/MAPK 신호전달 경로의 단백질 발현양상 변화 21 D. ERK …
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Scaffold-Mediated Organization of Signal Transduction Networks
… can serve as specificity determinants in Ras/MAPK signaling, and may function to coordinate the activity of this cascade with additional regulatory pathways required for an appropriate biological response. Thus, we provide evidence for functional diversification both at the level of Raf …
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Insights into Neuroblastoma Initiation and Disease Progression Through integrative Genomics and Epigenomics
… a strong enrichment in genes associated with RAS-MAPK signaling (18 of 23 patients). These RAS-MAPK mutations were clonally enriched at relapse and exist within clonal or major subclonal tumor populations. Similar MAPK pathway mutations were detected in 11 of 18 human neuroblastoma-derived …
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Ras Drives Malignancy Through Stem Cell Crosstalk with the Microenvironment
… (SCCs) are triggered by marked elevation of RAS/MAPK signaling and progression from benign papilloma to invasive malignancy. A subset of tumor-initiating basal progenitors, the cancer stem cells, obtain increased resistance to chemo and immunotherapy along this path. However, the distribution …
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Translational mechanisms of stem cell fate regulation in epidermal oncogene tolerance
… has not been explored. This is exemplified in RASopathies, where patients rarely develop epidermal defects or cancers despite germline mutations in the RAS/MAPK signaling pathway. Here, we find that oncogenic RAS-induced progenitor cell hyperproliferation is coordinated with differentiation to …
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Investigating the Mechanisms of Resistance to Dual PI3K/MTOR Inhibitor in PIK3CA Mutant Basal Like Bladder Cancer
… of alternative feedback signaling such as RAS-MAPK pathway. Based on the mechanistic changes induced upon resistance, we tested different drug combinations that can overcome resistance to PI3K targeted inhibition. Interestingly, we observed bromodomain inhibition by JQ1 to be the most …
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Genetic analysis of programmed cell death in Drosophila melanogaster
… is post-transcriptionally down-regulated by the Ras/MAPK pathway. This is consistent with the pro-survival activity of this pathway and is probably mediated by direct phosphorylation of Hid. Furthermore, analysis of mutations in the gene encoding the Drosophila IAP, Diapl, led to a model for how …
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Notch Signaling in Human Neuroblastoma Cells
… Notch target Hes-1 can be directly regulated by Ras/MAPK signaling at both normoxia and hypoxia, without the activation of Notch receptors.
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The Role of p66Shc in Stem Cells: Signalling, Identity and Apoptosis
… the canonical isoforms p52Shc and p46Shc act as Ras–MAPK transducers, the longer p66Shc isoform harbours an additional CH2 domain that confers distinct regulatory properties. Despite long-standing associations with oxidative stress and apoptosis, the physiological roles of p66Shc in stem cells …
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Reiterative FGF signaling determines the identity and morphology of the lacrimal gland
… that Shp2 is recruited by Frs2α to activate Ras-MAPK signaling downstream to Fgfr1 and Fgfr2 but not to Pdgfrα in the neural crest. By differential gene expression analysis, we identified homeodomain transcription factor Alx4 as the key effector of Shp2 signaling to control expression of …
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The Role of The Diras Family Members In Regulating Ras Function, Cancer Growth and Autophagy
<p><em>DIRAS3 </em>is a maternally imprinted tumor suppressor gene that is downregulated by multiple mechanisms across several tumor types. When re-expressed, DIRAS3 decreases proliferation, inhibits motility, and induces autophagy and tumor dormancy. <em>DIRAS3</em> encodes a 26 kDa small GTPase …
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Direct Effects of Metformin On Pi3K and Ras Signaling In Endometrial Cancer
… effect on cell proliferation, PI3K and Ras-MAPK signaling, and apoptosis. A xenograft mouse model was also used to evaluate the effects of metformin treatment on <em>in vivo</em> tumor growth. These preliminary studies demonstrated that K-Ras mutant cell lines exhibited a decreased …
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Characterization of Drosophila Ctr1a: New Roles for Ctr1 Proteins and Copper in Physiology and Cell Signaling Pathways
… a genetic interaction between Ctr1A and Ras was observed. Genetic experiments in Drosophila and cell culture experiments in both Drosophila and mammalian cell lines demonstrate a conserved role for Ctr1 proteins and copper as positive modulators of Ras/MAPK pathway signaling. Immunoblot …
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Rapid Functional Dissection of Genetic Networks Via RNAi In Mouse Embryos
… of the mammalian epidermis in vivo. Using ultrasound-guided in utero injections of fluorescently-traceable lentivirus particles carrying shRNA or Cre-recombinase into mouse embryos, I demonstrated a highly efficient, non-invasive, selective transduction of surface epithelium. Epidermal …
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