Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"Rap1"”.
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The Roles of Rap1 in Cancer Metastasis and Pancreatic Islet Beta Cell Function
<p>Signaling from the G protein, Rap1 is involved in several fundamental biological processes. Yet the mechanism or even consequence of Rap1 signaling in several biologies and diseases is still unclear. Rap1 has been implicated in cancer tumorigenesis, but its role in cancer invasion and metastasis …
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Rasa3 controls turnover of endothelial cell adhesion and vascular lumen integrity by a Rap1-dependent mechanism
… fait partie de la famille GAP1 qui cible Ras et Rap1. Bien que l’inactivation catalytique ou la délétion de Rasa3 en souris résulte en de nombreuses hémorragies et à la létalité embryonnaire, la fonction biologique sous-jacente à ces défauts reste inconnue. Ici, via une combinaison d’approche in …
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Investigating Mechanisms of Telomere End-Protection
… of the conserved shelterin component, Rap1, to telomere endprotection. Rap1 was first discovered in budding yeast as a transcription factor and was later shown to bind directly to telomeres. Two important functions of Rap1 in yeast are: the negative regulation of telomere length, and …
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Identification and Characterization of the DNA Damage Response Function of Human Rif1 and a Search For Human Rap1 Interacting Factors
<p>Rifl, the ortholog of the yeast Rapl interacting factor 1, a telomere length regulator was identified. Immunofluorescence did not reveal an association of human Rifl with telomeres. Preliminary co-immunoprecipitation with known telomeric proteins and chromatin immunoprecipitation failed to show …
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The crosstalk between the ERK and the cAMP signalling pathways in PC12 Cells
… and in response to cAMP, the activity of Ras and Rap1 were measured by affinity pulldown assays. Upon EGF the signal was transduced through Ras only, whereas upon NGF the signal was mediated through both Ras and Rap1. cAMP sensitised Rap1 that became activated upon EGF stimulation indicating that …
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Investigation of T cell signalling events regulating immunity and tolerance in vivo
… p38 MAPK signalling cascades. The small GTPase, Rap1, has long been implicated in such desensitisation of ERK, and the consequent reduced IL-2 production, observed in tolerised T cells. However, as noted above, the majority of these findings were obtained from in vitro studies of T cell lines or …
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Molecular mechanisms of signal transduction in neutrophil chemotaxis
… of Lyn. We documented the small GTPase Rap1 as a major downstream effector of Lyn to regulate neutrophil adhesion during chemotaxis. Depletion of Lyn abolished chemoattractant-induced Rap1 activation at the cell's leading edge. Furthermore, Lyn controls spatial activation of Rap1 by …
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The Apollo Nuclease Binds To TRF2 and Protects
… complex with the telomere binding proteins TRF2/Rap1. Due to its low abundance at telomeres, we consider Apollo to be a telomere accessory factor, and not part of the core telomere protein complex, shelterin. Apollo localizes to telomeres by interacting directly with the TRFH domain of TRF2. …
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Mechanisms of Telomere End Protection by TRF2
… of shelterin, TRF1, TRF2, TIN2, TPP1, POT1, and Rap1, each have distinct roles in telomere homeostasis. These include telomere length regulation and preventing telomeres from activating various DNA damage response (DDR) signaling and double-strand break repair pathways. The need to suppress these …
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Funktionelle Charakterisierung des Vasodilatator stimulierten Phosphoproteins (VASP) für die Stabilität des Aktin-Zytoskeletts und die Integrin-abhängige Zelladhäsion
… eine VASPAbhängigkeit dieses Effekts. Der GTPase Rap1 kommt eine wichtige Bedeutung bei der Integrin-abhängigen Adhäsion von Zellen zu. Aktivierung von Epac, einem Guanin-Nukleotid- Austauschfaktor von Rap1, führt in Wildtyp-Zellen zur Verstärkung des Widerstandes gegenüber mechanischen Kräften, …
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The role of adaptor proteins Crk and CrkL in lens development
… to downstream effectors, including Ras, Rac1 and Rap1, which are important for cell proliferation, adhesion and migration. Underlying their diverse function, these two adaptor proteins have been implicated in receptor tyrosine kinase signaling, focal adhesion assembly, and cell shape. To explore …
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Analysis of Nitrogen starvation induced filamentous growth and characterization of putative essential genes in the human fungal pathogen, Candida albicans
… dieser Arbeit funktionell charakterisiert. RAP1 codiert für das Repressor/Aktivator Protein 1, ein Transkriptionsfaktor und Telomerbindeprotein, das in der Bäckerhefe Saccharomyces cerevisiae essentiell ist. Die Deletion des RAP1 Gens in C.albicans beeinträchtigte jedoch nicht die …
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Determining the differential roles of the dock family of GEFs in drosophila development
… evidence that Spg functions in concert with Rap1 to regulate aspects of adhesion. Together these data show that Mbc and Spg can have differential downstream GTPase targets. Our findings predict that the ability to regulate downstream GTPases is dependent on cellular context and allows for the …
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Regulation of LFA-1 on T cells by phosphoinositide signalling
… LFA-1-mediated adhesion to ICAM-1, including the RAP1/RAS GTPase-activating protein RASA3. I found that RASA3 is a critical negative regulator of LFA-1 activation and that RASA3 is inhibited by PI3K signalling. T cells without RASA3 have greatly increased ICAM-1-binding. Mice with conditional …
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Application of NMR spectroscopy to interrogate the structures of bacterial protein toxin membrane localization domains and amphotericin B
… expressed by Pasteurella multocida, and the Ras/Rap1-specific endopeptidase domain of the multifunctional autoprocessing repeats-in-toxins (MARTX) toxin expressed by Vibrio vulnificus. The function of these MLDs is to localize the toxin to membranes, which aid in the targeting of the catalytic …
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Mechanisms of platelet inhibition by the selective serotonin reuptake inhibitor citalopram
… agonist U46619, despite blocking subsequent Rap1 activation. A credible target for this inhibitory mechanism is the calcium and diacylglycerol guanine nucleotide exchange factor-1 (CalDAG-GEFI): 2) citalopram suppressed early protein phosphorylation within the GPVI pathway, resulting in the …
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Transcriptional Regulatory Mechanisms of Ribosomal Protein Genes
… of histone H4) complex to activator Rap1. NuA4 enhances PIC (Pre-initiation complex) formation at the core promoter, but it is not clearly understood how does it do so. Researches have identified two different forms of TBP: TAF (TBP associated factor)-dependent form of TBP and …
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Hypoxic immunomodulation results in increased disease risk and altered behavior via non-canonical pathways
… genes. Disease pathways signaling through rap1 signaling, natural killer cell mediated cytotoxicity, and asthma were over-represented within the interior tactic. These results indicate that overhaul exposure after an interior tactical choice show immunosuppression with the advancement of …