Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"Randomly Amplified Polymorphic DNA"”.
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The optimisation of protocols for the extraction of nuclear DNA from animal bone forPCR.
… were to find an effective method for extracting DNA from bone and to use this methodology to create Randomly Amplified Polymorphic DNA- Polymerase Chain Reaction (RAPD-PCR) profiles of different species. A modified Chelex method was selected as the preferred extraction procedure and RAPD-PCR …
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Impact of a Historical Translocation on Populations of White-Tailed Deer Odocoileus virginianus
<p>Randomly amplified polymorphic DNA (RAPD) analysis was used to study the impact of a historical translocation event on populations of white-tailed deer in Georgia. The technique may also serve as a model by which management practices for other rare species can be studied. Populations from an …
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Genetic Diversity and Camptothecin Variation in Camptotheca Decaisne
… primers (OPA02, OPA03, and OPA04), generating 44 polymorphic bands using randomly amplified polymorphic DNA (RAPD) markers, were able to discriminate among 25 Camptotheca populations. The band size varied from 268-4,411 bp, with an average of 15 bands/primer. Of these populations, 'Katie', HJ …
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Morphological, Molecular, and Biogeographical Variation within the Imperiled Virginia Spiraea
… at the molecular level as examined with Randomly Amplified Polymorphic DNA (RAPDs) and band patterns were used to construct a cluster analysis. Past gene flow was identified by combining cluster analysis and biogeography data. Results support the current species delineation by affirming …
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Effect of Pollution on Genetic Diversity in the Southern California Bight
… was performed to measure single-strand DNA breaks in mussels at several of the sites in order to confirm differing impacts of pollution on the animals at the different sites. In this assay, cells in impacted populations with increased DNA damage displayed increased migration from the …
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The prevention and diagnosis of central venous catheter-related infection
… of staphylococci was evident whilst PFGE and randomly amplified polymorphic DNA (RAPD) were highly discriminatory for the molecular characterisation of S. aureus and CoNS. This was highlighted in 8 stem cell transplant (SCT) patients whereby it was demonstrated that routine identification and …
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Studies of androgenic processes in diploid potato
… a better regeneration protocol is developed. Randomly amplified polymorphic DNA (RAPD) techniques were applied to analyze the genetic composition of anther-derived plants, whose ploidy level was predetermined by flow cytometry. The RAPD fragments amplified from various anther derived diploid …
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Systematics and reproductive biology of the genus Morus L. (Moraceae)
… spacer (ITS) region of the nuclear ribosomal DNA and the trnL-trnF region of the chloroplast DNA to reconstruct the evolutionary history of Morus. The phylogenies were congruent and indicate a) a monophyletic core group of Morus with two well-supported geographical clades (one containing Asian …
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Studies of verticillium wilt and characterization of candidate verticillium wilt resistance genes in the mint species Mentha longifolia (L) Huds
… diversity assessment was conducted utilizing randomly amplified polymorphic DNA (RAPD) markers. The accessions were found to be diverse regarding all observed traits and the South African accessions in particular were shown to possess unique features. Most importantly, highly wilt-resistant …
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Genetic Characterization of Zambian Native Cattle Breeds
… Tonga, and Baila based on body measurements and randomly amplified polymorphic DNA (RAPD) markers, respectively. A total of 100 animals, 25 from each of the four breeds associated with different tribes and region of Zambia, were used in the molecular analysis research. Additionally, 10 Holstein x …
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Determining Sources of Fecal Pollution in Water for a Rural Virginia Community
… for fecal source identification, followed by randomly amplified polymorphic DNA (RAPD) analysis for confirmation. Millwood consists of 66 homes, all served by individual septic systems, and a stream (Spout Run) passes through the center of the community. Spout Run drains a 5,800 ha karst …
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The relationship between fruit color traits and anthocyanin genes in diploid Fragaria
… in the anthocyanin biosynthetic pathway were amplified, cloned and sequenced. Intron length polymorphisms for each of these genes were detected among three diploid varieties: F. vesca Alpine variety 'Yellow Wonder' (YW) (Europe); DNIC, an F. vesca clone collected from Northern California; and …
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The evolution of flat periwinkles Littorina fabalis and L. obtusata emhasizing mitochondrial introgression and restricted recombination
… also in the protein arginine kinase (Ark) and a Randomly Amplified Polymorphic DNA (RAPD) locus and this linkage disequilibrium persists in locations where both ecotypes are present suggesting that recombination is strongly suppressed between Ark, the RAPD locus and one or several loci …
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Molecular marker analysis of a segregating monoploid potato family
… Simple sequence repeat analysis with eight polymorphic primer pairs was used successfully to identify the homozygous diploid plants that were added to the monoploids. In total 34 monoploid plants and 14 homozugous diploids were obtained. The degree of heterozygosity revealed by SSR analysis …
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Molecular Systematics of the Entomopathogenic Bacteria Bacillus popilliae, Bacillus lentimorbus, and Bacillus sphaericus
… isolates of these bacteria were examined for DNA similarity. Three distinct but related similarity groups were identified; the first contained strains of B. popilliae, the second contained strains of B. lentimorbus, and the third contained two strains distinct from but related to B. popilliae. …