Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 21 for “"Random Mutations"”.
-
Uncovering the Critical Amino Acids in the Beta Bubunit of the Epithelial Sodium Channel (ENaC)
… chain reaction (EP-PCR) was used to introduce random mutations in the extracellular loop of the beta subunit of ENaC in order to identify residues that may be critical to proper function. A yeast screen, dilution survival pronging assay, was used to visualize the level of growth inhibition of …
-
A Study of Evolutionary Contingency: Characterisation, Mechanisms, and Evidence
… key candidate sources of contingency: random mutations, recombination, genetic drift, species sorting, and mass extinctions. I conclude that random mutations fail to be a strongsource of contingency as they are often biased towards particular mutational outcomes on account of certain …
-
Digital signatures to ensure the authenticity and integrity of synthetic DNA molecules
… physical DNA molecule can affect its properties, random mutations can accumulate in DNA samples over time, DNA sequencers can sequence (read) DNA erroneously and DNA sequencing is still relatively expensive (which means that laboratories would prefer not to read and re-read their DNA samples to …
-
Role of Nudix-Hydrolase ASMTL in Modulating Mitochondrial Biogenesis of Cancer Cells
… into newly synthesized DNA, resulting in random mutations or, when overwhelming into DNA damage, culminating into apoptosis, which is the desired effect of anti-cancer chemotherapy. Human cells contain nucleotide sanitation enzymes like ASMTL, which prevent incorporating the non-canonical …
-
Evolution of the Proteasome
… activity or restricted gene expression caused by random mutations to coding or regulatory elements of duplicated genes. The neutral theory is not supported by these results because unprocessed proteasome pseudogenes are not found. Finally, scoring tables calculated from numerical indices of amino …
-
On the nature and measurement of variational bias: a developmental perspective
… modelings that the phenotypic production from random mutations is isotropic and uniform. Conventional methods linking genetic variants and phenotypic variation often assume that the origin of phenotypic variation is purely due to genetic and environmental factors. Here, in this thesis, I adopt …
-
Screen for Suppressors and Enhancers of Excitotoxic Neurodegeneration
… genome. b) We are using EMS to introduce random mutations throughout the genome. In both cases we are monitoring the effect of genetic modification on the level of neurodegeneration. We have screened ~2,000 mutagenized genomes and identified 2 mutant strains that show enhanced …
-
Cooperative assembly confers regulatory specificity and promotes evolution
… In a cooperatively regulated circuit, random mutations to the DNA will be preserved if they initiate or improve a regulatory connection with a stabilized TR, and will be identified by DNA sequencing. This simplified synthetic approach in a controlled culture environment will elucidate …
-
USING A NOVEL CELL- PENETRATING PEPTIDE TECHNOLOGY TO INDUCE PLURIPOTENCY
… can have undesirable off-target effects, e.g. random mutations in the genome. A safer approach to inducing pluripotency is exogenous delivery of OSKM proteins using cell-penetrating peptides (CPPs). Classically, CPP-mediated delivery has been inefficient because of poor penetration and …
-
Transcription Factor-Centric Approaches to Identify Regulatory Driver Mutations in Cancer
… previous efforts to identify cancer driver mutations have focused on protein-coding genes. In recent years, the decreasing costs of DNA sequencing have enabled whole-genome sequencing (WGS) studies of thousands of tumor samples, making it possible to systematically survey non-coding regions …
-
Maternal environment and offspring physiology : the inheritance of information across a generation
… resulting from sexual reproduction and random mutations. Nonetheless, throughout the 20th century there have been several observations of plants and animals where parental exposure to environmental stress modified offspring physiology. These changes in progeny physiology sometimes …
-
Mining biological diversity to optimize chlorite dismutase for water treatment
… This could be further improved by generating random mutations in the sequence of Cld and then applying directed evolution to select for beneficial mutations. If successful, this type of engineering could also further the limited understanding of the mechanisms by which Cld inactivates. In …
-
Site-Directed Mutagenesis in Francisella Tularensis by Allelic
… type A and type B stains. However, site-specific mutations remain difficult to execute or these methods generate random mutations. In this study a novel method was developed to create site-directed mutations in a putative capsule biosynthesis locus to knock out encapsulation of the attenuated F. …
-
PATTERNS OF NATURAL SELECTION ASSOCIATED WITH TROPISM SHIFTS IN ANIMAL CORONAVIRUS GENOMES
Viruses may acquire mutations that result in a tropism shift. RNA viruses, such as Coronaviruses (CoVs), are susceptible to tropism shifts. A tropism shift occurs when a virus alters the tissue or cell type it infects, which can have important implications for disease pathogenesis, virulence, …
-
A Forward Genetic Screen Revealed the Role of POLDIP2 in Ensuring Maternal Inheritance of Mitochondrial DNA in Drosophila melanogaster
… I examined 1415 mutant lines carrying random mutations in the nuclear genome (on the 3rd chromosome). This work involved dissecting and staining seminal vesicles with an antibody targeting dsDNA to quantify mtDNA levels in mature sperm. Through the screen, I identified 63 lines that …
-
Protein structure prediction and structure-based protein function annotation
… our understanding of the bioverse. However, random mutations occurring during evolution chisel the sequence to an extent that both decrypting these codes and identifying evolutionary relatives from sequence alone becomes difficult. Thankfully, even after many changes at the sequence level, …
-
Investigating mitochondrial dynamics in Drosophila spermatogenesis: a focused study on the mitoball
… melanogaster* with EMS, a mutagen that induces random mutations in the nuclear genome, and visualising mitoballs by confocal imaging. This screen identified 121 lines with abnormal mitochondrial and testis morphology. Focusing on nine EMS lines with mitoball defects, I performed whole genome …
-
Structure-function analysis of IntDOT, the CTnDOT integrase
… mutants from a large population containing random mutations. I isolated twenty five IntDOT mutants that are defective in integration. Four substitutions contained substitutions int eh N domain, two contained substitutions in the CB domain, and the nineteen contained substitutions in the CAT …
-
Application of High Resolution Melting analysis for haplotype detection in phylogeographic research and case studies of Arenaria ciliata, A. norvegica and Minuartia recurva (Caryophyllaceae)
… Based on an amplicon of rps16I in A. ciliata, random mutations are made and HRM sensitivity is evaluated to different classes of single nucleotide substitutions. Class I and II class I (A/G or C/T) and II (A/C or G/T) substitutions are demonstrated easier to be detected than class III (C/G) and …
-
Female sterility and carpel development in plants
… evolves from hermaphroditism by the process of random mutations affecting the female organ (carpel) or male organ (stamen) abortion. The ABCE model of floral development provides a basic underlying developmental framework of individual floral whorls across species. However, it stops short of …
Page 1 of 2