Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 37 for “"Random Coil"”.
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Covalent Modification that Enhances Protein Properties and Functions and Folds Random Coil into Alpha-Helix
… modify proteins covalently to induce folding in random coil sequences, prevent aggregation, surface adsorption and for increasing the thermal stability against aggregation of proteins.</p> <p>The first chapter describes the chemistry for modifying the lysine residues in proteins with alditols …
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Characterization of structure and dynamics of membrane proteins from solid-state NMR
… virus shedding. The M2(22-71) construct shows random-coil chemical shifts, large motional amplitudes, and a membrane surface-bound location with close proximity to water, indicating the post-amphipathic helix (AH) cytoplasmic domain is a dynamic random coil near the membrane surface. The …
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Thermodynamics and Kinetics of Iso-1-cytochrome c Denatured State
… non-foldable protein sequences behave like a random coil. However, heteropolymeric foldable sequences have scaling factors higher than those of a random coil, suggesting the presence of residual structure in denatured proteins. Thus, peptide models with homopolymeric sequences do not …
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Thermodynamics and Kinetics of Iso-1-cytochrome c Denatured State
… non-foldable protein sequences behave like a random coil. However, heteropolymeric foldable sequences have scaling factors higher than those of a random coil, suggesting the presence of residual structure in denatured proteins. Thus, peptide models with homopolymeric sequences do not …
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Long peptides for cysteine arylation
… we discovered that both mixed alpha-helical and random coil as well as beta-sheet-based reactive peptides existed. Studying their structural properties revealed that many of the peptides undergo significant structural alterations upon reaction with the perfluoroaromatic. Returning to MP01 we …
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Structural Investigations of Nematode Anhydrobiotic and Rab11-FIP Proteins by Far-UV Circular Dichroism and Mid-IR Fourier Transform Infra-Red Spectroscopy
… to be very accurate for estimating a-helix and random coil content, but considerably less so for estimating b-sheet. Conversely, FTIR spectroscopic analysis of the same proteins proved to be more accurate for b-sheet estimation than either a-helix or random coil. Overall, CD spectroscopy was …
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Investigation of the interaction of GdnHCl with poly-L-lysine, horseradish peroxidase and cytochrome c monitored via FTIR, CD and fluorescence spectroscopies
… studies of protein chemical denaturation. PLL, a random coil, was used to model the interaction between Gdn-d 5 -DCl and random structures. Spectroscopic evidence strongly suggested that Gdnd 5 -DCl associates with PLL, particularly with its lysyl side chains. The analysis of HRP indicated that it …
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Conformational and Orientational Dynamics of Semi-Rigid Macromolecules in Shear Flow
… exists as an $\alpha$-helix at high pH and a random coil at neutral pH. When the $\alpha$-helix is heated above 27$\sp\circ$C, the macromolecule undergoes a conformational transition to a $\beta$-sheet. In this study, the stability of the secondary structure of poly-L-lysine in solutions …
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Diffusion of a rodlike virus in complex solutions
… Tobacco Mosaic Virus (TMV), through solutions of random coil and globular polysaccharides, taken separately. The polymers are effectively invisible in the depolarized scattering experiment allowing only the TMV probe to be seen. Also described is the use of fluorescence photobleaching recovery to …
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Conformation of Transmembrane Segments of a Protein by a Coarse Grain Model
… connectedness in the low-temperature region and random coil (D~2) and then linear conformation (D~1) on increasing temperature further.</p>
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Spectroscopic Analyses Of Factors Affecting Formation And Stability Of I-Motif Dna
… a mechanism is proposed for the folding of a random coil oligo into the iM. The fluorescence changes in tC° describes the hydrogen bonding of the cytosines that occur during the folding of the iM. This folding mechanism shows that all hydrogen bonds form on the same time scale as the iM forms, …
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Frustration of protein folding from in vitro to in vivo
… and vital biological process, where protein random coil transforms into certain conformation in order to fulfill its function. Misfolded protein which fails to acquire proper shape, not only loses its function, but can also cause fatal diseases. In this dissertation, I will present four case …
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Inverse Monte Carlo simulation of biomolecular conformation and coarse-grained molecular modeling of chondroitin sulfate conformation, titration, and osmotic pressure
… the ordered globule, disordered globule, and random coil states. In the second part of this thesis, a molecular model is developed and used to investigate the properties of anionic glycosaminoglycan (GAG) molecules. GAGs are critically important to the structure and biomechanical properties of …
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Conformational Analysis of Disordered Proteins Using H/D Exchange Mass Spectrometry
… two classes of IDPs: the molten globule and random coil, respectively. The mutual synergistic folding phenomenon observed when a complex is formed between these two proteins was also analyzed. A lower limit of several seconds of D2O exposure time is imposed when manual pipetting is used to …
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Phenylene Ethynylene Foldamers: Cooperative Conformational Transition, Twist Sense Bias, Molecular Recognition Properties, and Solid -State Organization
… The conformational transition from a random coil to helical conformation was quantitatively characterized by fluorescence spectroscopy. From these measurements, it was determined that the stability of the helical conformation is linearly dependent on chain length. The addition of …
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X-Ray crystal structure of a ribosomal protein S6, S15, S18 : rRNA complex from T. thermophilus, and investigation of conformational changes in 16S rRNA fragments from B. stearothermophilus during ribonucleoprotein assembly
… structured protein with both a-helices and random coil elements. The S6:S18 heterodimer binds across the helix 22, helix 23, helix 23a RNA junction and makes no direct contacts to S15. Time-resolved fluorescence resonance energy (trFRET) methods were used to assess conformational changes in …
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The Interactions for the RBP Receptor, Stimulated by Retinoic Acid Gene 6
… STRA6-ICL3 was mostly composed of β-sheet and random coil. Crystallization trials were also attempted and a crystal is eagerly awaited.
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Expression of cysteine-rich pig gastric mucin (Muc5AC) domains in Pichia pastoris and their pH-dependent properties
… slight decrease in α-helix content and increased random coil or β-strand for the C-terminal and Cys-repeat peptides respectively. Thus, decreasing pH causes an increase in the exposure of, and binding to, hydrophobic sites of recombinant peptides from the Cys-rich regions of PGM. The increased …
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THERMODYNAMIC PROPERTIES OF THE UNFOLDED ENSEMBLE OF PROTEINS
A random coil, whose size is determined by its excluded volume, and net energetic interactions with its environment, has served as a representation of the unfolded ensemble of proteins. The work in this thesis involves equilibrium, nuclear magnetic resonance and time-resolved kinetics spectroscopic …
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Extending Students' Practice of Metacognitive Regulation Strategies in the Undergraduate Chemistry Laboratory and Investigation of Pb2+ Binding to Calmodulin with Circular Dichroism and Molecular Dynamics Modeling
… indicated that α-helical structure decreased and random coil structure increased when CaM was saturated with Pb<super>2+<super> compared to Ca<super>2+<super> saturated CaM. A molecular dynamics simulation of Pb<super>2+<super> binding to CaM showed that Pb<super>2+<super> ions bound to sites …
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