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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"Raman microspectroscopy"”.
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Single-cell analysis of hematopoietic cell fate on niche-mimetic platforms using Raman microspectroscopy
… combinations on stem cell fate specification. Raman spectroscopy has emerged as one of the few analytical approaches that can be used to noninvasively assay the biomolecular composition of cells. Combining Raman spectroscopy with multivariate methods such as partial least-squares discriminant …
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Effects of Alpha-Tocopherol Antioxidant on the Bonding Properties of Resin Adhesive to Dentin Bleached with Sodium Perborate
… specimens were further evaluated with Raman microspectroscopy for depth of penetration and degree of conversion of adhesive resin. Mean bond strength values (MPa) for each group: unbleached control=26.2, bleached control=20.3, post-bleach treatment group=18.5. A 1-factor ANOVA and Tukey …
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Toward antiquity-inspired design in materials and construction: Insights into the production and durability of the ancient materials Egyptian blue and Roman concrete
… tomography, synchrotron x-ray diffraction, Raman microspectroscopy, scanning electron microscopy and thinsection petrography, map complex, heterogeneous dissolution processes throughout the cementing matrix of mortar samples. Samples from the Tomb of Caecilia Metella (First Century BCE) …
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Raman spectroscopic characterisation of inorganic fibres and particles and their coverage by wetting agents.
… of fibres is then considerably reduced. Raman microspectroscopy has been shown to be a very powerful technique for the identification of micrometer-sized fibres and particles, with little or no sample preparation. Such spectra are sensitive to the composition of the material and can often …
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Spectroscopic Study of Compressible Mobile Phase and Stationary Phase Behavior in Chromatography
Raman spectroscopy, laser-induced fluorescence, and sum-frequency generation (SFG) spectroscopy are used to investigate the behavior of compressible mobile phases and stationary phases under a variety of chromatographic conditions. Efforts to understand and optimize separations employing …
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Methanogens from Siberian permafrost as models for life on Mars : response to simulated martian conditions and biosignature characterization
… to characterize their biosignatures by means of Raman spectroscopy, a powerful technology for microbial identification that will be used in the ExoMars mission. For this purpose, methanogens from Siberian permafrost and non-permafrost habitats were subjected to simulated martian desiccation by …
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Effect of Final Irrigation on Sealer-Dentin Interface for a Methacrylate-Based Resin Sealer
… apical specimens. Specimens were analyzed using Raman microspectroscopy, Goldner’s trichrome stain, and SEM. There was no difference in the quality of the intertubular sealer-dentin interface (as measured by hybrid layer production and sealer degree of conversion) or dentinal tubule sealer …
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Optimising nutrient use efficiency in crop production
… infrared (FTIR) spectroscopy and Raman spectroscopy are two such approaches and have been readily implemented across biological samples. However, their applications in the field of plant science have been relatively underexploited. This is largely associated with the presence of …
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Effects Of Delayed Polymerization Time and Bracket Manipulation on Orthodontic Resin Modified Glass Ionomer Adhesive
… be the first study to quantify DC of RMGI using Raman microspectroscopy, several challenges, including weak peak signal with and without fluorescence, were encountered and as a result, DC could not be determined. A significant difference (p<0.05) in ARI score was detected between the 0.5-min and …
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Development of single-cell Raman spectroscopy for noninvasively screening the differentiation of living stem and progenitor cells in biomaterial platforms for 2D and 3D cell culture
… applications. The work presented here uses Raman microspectroscopy to analyze the differentiation stages of single stem and progenitor cells in 2D and 3D cell cultures. This approach needed the development of a custom Raman-compatible substrate, presented in Chapter 2, to be used as an …
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Methods for Improving Signal to Noise Ratio in Raman Spectra
Raman microspectroscopy is an optoelectronic technique based on the inelastic scattering of light. This technique has been demonstrated to have potential to identify different materials based on subtle differences in the Raman spectral profile using various multivariate statistical classification …
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Diffuse Damage Repair Mechanism in Bone
… deposition in repair of diffuse damage</p> <p>Raman microspectroscopy studies showed no difference in mineral/matrix, carbonate/phosphate ratio and crystallinity among diffuse damage, control and repaired diffuse damage tissue or survival groups. Raman microspectroscopy studies revealed a weak …
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Altered Cholesterol Metabolism In Human Cancers Unraveled By Label-Free Spectroscopic Imaging
… in cancer cells.</p> <p>Enabled by label-free Raman spectromicroscopy, we performed the first quantitative analysis of lipogenesis at single cell level in human patient cancerous tissues. Our imaging data revealed an unexpected, aberrant accumulation of esterified cholesterol in lipid droplets …
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The surface characterisation of pharmaceutical mini-tablets using thermal probe techniques
… confirming these initial results. Additionally, Raman microspectroscopy was performed on the mini-tablets as a corroborative technique, this method being based on a different physical phenomenon. In conclusion, the thermal probe techniques (micro-TA, nano-TA and TTM) were shown to be sufficiently …
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The Impact of substrate texture on cell behavior
… We designed setups that allow us to perform Raman microspectroscopy both in 2D and 3D topographical patterns in order to be able to study their morpho-chemical effects on cells. We also genetically modified 3T3 fibroblasts to express fluorescent-tagged proteins that visualize the actin …
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Vibrational spectroscopy studies of interdiffusion in polymer laminates and diffusion of water into polymer membranes.
Confocal Raman microspectroscopy has been used to study the interdiffusion in polymer laminates at the interfacial region between the constituent polymer layers. The effects of the polymer molecular weight, annealing temperature, and annealing time have been studied. Three different laminates with …
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Effect of Radiotherapy on Nano-Mechanical Properties and Chemical Composition of Tooth Enamel and Dentin
… was used to evaluate modulus and hardness, while Raman microspectroscopy was used to measure protein/mineral ratios, carbonate/phosphate ratios, and phosphate peak width. All measures were completed prior to and following in vitro simulated radiation and after in vivo radiation at the same four …
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Utilizing Raman spectroscopy and multivariate analysis to identify and monitor the differentiation states of individual stem cells
… herein investigates the potential of spontaneous Raman microspectroscopy as an objective, non-invasive, marker-free, quantitative technique to chemically characterize and identify individual cells, specifically stem cells and their progeny, with location and time specificity. Chapter 1 presents an …
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Uncovering the role of biomineralisation in otolith trace element uptake by microstructural and microchemical analysis
… organic matrices orientation, as determined by Raman microspectroscopy, in the otoliths of Platycephalus bassensis, Chrysophrys auratus, Trachurus novaezelandiae and Lates calcarifer. The orientation results indicate that otolith mineral growth is directed and controlled by the organic matrix, …
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