Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"RalA"”.
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Enhancing the radio sensitivity of prostate cancer using RALA Gold nano-complexes
… This thesis employs the cell penetrating peptide RALA to enhance AuNP cell internalisation and in turn radiosensitivity of PCa. RALA-AuNP have undergone extensive characterisation following synthesis, proving optimal for in vitro and in vivo application. Significant improvements in cell uptake and …
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Investigating the C-terminal region of RalA: regulation through membrane dynamics and protein-protein interactions
RalA and RalB, members of the Ras superfamily, play a pivotal role in tumour progression in Ras-driven cancers, hence there is a growing need to understand these proteins in terms of their structure and function to facilitate future pharmaceutical intervention in this critical oncogenic pathway. So …
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The Development and Characterisation of a Peptide Delivery Platform to Interrogate the Anticancer Effects of Bisphosphonates
The RALA peptide delivery system complexes anionic compounds through electrostatic interactions, to form a nanoparticle with the physiochemical characteristics to cross cellular membranes and escape endosomes; thereby releasing the cargo intracellularly to exert the optimal therapeutic effect. …
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Understanding the biological barriers to Jurkat Transfection using non-viral peptides
… of this thesis is to examine the feasibility of RALA peptide to manufacture CAR-T cells. <br/><br/>As primary T-cells are hard to transfect, the key barriers to the transfection of primary T-cells should be explored for further rational designs of transfection agents. The low level of heparin …
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Molecular regulation of neuroblast migration in the postnatal brain
… CB1 and CB2, leads to significant activation of RalA,<br/>a Ras-like GTPase involved in the control of neuronal morphology and polarity.<br/>siRNA-mediated knockdown of RalA or the expression of dominant negative RalA<br/>abolished cannabinoid-induced stimulation of migration. Time-lapse …
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UNDERSTANDING THE RAL GTPASES: THE REGULATION BY PROTEIN LYSINE FATTY ACYLATION, SIRT2, AND INTERACTING PROTEINS
… understanding two highly similar small GTPases, RalA and RalB. They are the two members of the Ras-like (Ral) GTPases in the Ras subfamily. Despite their similar biochemical and structurally properties, the Ral GTPases can exhibit diverging roles. My thesis work provides important insights into …
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Development of an inhalable nanomedicine for lung cancer
… the stability and functionality of NPs. Herein, RALA peptide that had been successfully incorporated into different formulations, e.g. wound patch and hydrogel, was used to condense plasmid DNA (pEGFP-N1) into NPs, followed by spray drying to produce inhalable powder as a proof of concept of gene …
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Evaluation of Chronic RalGTPase Activation as a Core Specifier of Oncogenic Transformation
Ral (RAS-Like) GTPases, RalA and RalB, were originally identified based on sequence similarity to Ras and are directly activated via the Ras effector family Ral guanine nucleotide exchange factors (RalGEFs). Previous studies have demonstrated that RalA and RalB collaborate to maintain …
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The development of novel strategies to modulate epithelial sodium channel (ENaC) activity in chronic obstructive pulmonary disease (COPD)
… investigated the ability of the QUB-patented RALA peptide drug delivery system to transport an αENaC small interfering RNA (siRNA) cargo into primary bronchial epithelial cells (PBECs). RALA/siRNA nanoparticles were well-tolerated by submerged PBECs and transfection of cells led to a high …
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RalGDS-Dependent Cardiomyocyte Autophagy Is Necessary for Load-Induced Ventricular Hypertrophy
… isoforms of Ral that are downstream of RalGDS (RalA, RalB) and whose actions are mediated by the exocyst. In these experiments, mTOR inhibition was maintained in response to starvation and Torin 1 despite RalA or RalB knockdown; however, autophagy was diminished only in NRCM's with RalB …
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Caracterização da microbiota lática do Queijo Minas artesanal produzido na região do Serro, Minas Gerais, por métodos cultura dependente e independente
… alguns produtores da região do Serro utilizam “rala” como fermento endógeno, obtida pela raspagem do QMA após 3-5 dias de fabricação. A alteração do fermento endógeno pode levar a alterações da microbiota do QMA, incluindo a microbiota lática que exerce papel fundamental na produção, maturação, …
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Ras Intrinsic and Extrinsic Pathways in Human Cancer
… RalGEFs activate two other small GTPases, RalA and RalB. The role of these two proteins in Ras-mediated cancer was poorly understood. I thus assessed the requirement of RalA and RalB in tumor metastasis discovering that both proteins promote this critical step in cancer.</p><p>Ras does not, …
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Regulation of Weibel-Palade Body Exocytosis by alpha-Synuclein in Endothelial Cells
… WPB exocytosis through interference with RalA activation by enhancing the interaction of RalGDS/b-arrestin complexes. Immuno-EM analysis revealed that a-synuclein was localized close to WPB. These findings imply that a-synuclein plays as a negative regulator in WPB exocytosis in …
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The development and characterisation of MicroRNA and gemcitabine nanotherapies for the treatment of pancreatic cancer
… and intraindividual variability. Furthermore, RALA/GTP nanoparticles demonstrated potentiation of the cytotoxic effect of the chemotherapeutic agent by 8- to 24-fold compared to the parental drug.<br/><br/><i>Thesis embargoed until 31 July 2027.</i>
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Rab8 Directs Tubulation and Furrow Ingression During Epithelial Formation in Drosophila Melanogaster
… with Sec5, a subunit of the exocyst and RalA, a small GTPase implicated in directed membrane trafficking.</p> <p>Disruption of Rab8 function results in a complete failure of furrow formation in the early embryo, due to a failure of proper F-actin cytoskeletal dynamics and localization, …
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Development of stapled peptides targeting the Ral GTPases
… evidence implicating the Ral GTPases, RalA and RalB, which are activated downstream of Ras, as critical drivers of cell growth and metastasis in numerous Ras-driven cancers. Therefore, targeting this pathway may provide an effective method for inhibition of oncogenic Ras signalling. …
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The Mechanism of Tumorigenesis In The Immortalized Human Pancreatic Cell Lines: Cell Culture Models of Human Pancreatic Cancer
… and Her2 downstream effector pathways –MAPK, RalA, FAK, together with upregulation of cyclins and c-myc were involved in the malignant transformation. We discovered that MDM2, BMP7 and Bmi-1 were overexpressed in the tumorigenic HPDE cells, and that Smad4 played important roles in regulation …
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Evaluación sobre el aprovechamiento de Tillandsia usneoides (paste de montaña) en comarcas Lipululo y El Limito, Jinotega
… de árboles con características como copa rala y extendida de hojas pequeñas a medianas, con altura de 25 – 30 metros. Para estimar el incremento en longitud de las cortinas inicialmente se realizaron cortes a estas, luego se determinaron dos períodos de evaluación, a los cuatro y ocho …
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Saberes tradicionais: etnobotânica no contexto da área de Proteção Ambiental (APA) e área de relevante interesse ecológico (ARIE) da Barra do Rio Mamanguape-PB
… outros tipos de vegetação densa, vegetação rala, solo exposto e/ou área urbana, lavoura temporária e a classe água / nuvem e / ou sombra. Dentre as famílias botânicas citadas predominaram a Fabaceae, Anacardiaceae, Apocynaceae, Bignoniaceae e a Myrtaceae, enquanto as espécies que se …
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Análisis comparativo del estado forestal del bosque seco caducifolio en el refugio de vida silvestre Escalante-chacocente entre los años 1989-1994
… la regeneración predominaron en la categoría rala. La intervención antropogénica (Fuego, tala y pastoreo) a afectado seriamente al bosque ya que estos en los cinco años transcurridos se han incrementado (especialmente el fuego), el cual se manifiesta en la mayoría de los puntos muestreados, …
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