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Showing 1 to 20 of 86 for “"Radioimmunoassay"”.
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Radioimmunoassay and characterization of ligandin in the rat
Ligandin, a major soluble hepatic protein with the distinctive property of binding a wide range of organic anions, is a relative newcomer to the field of hepatic physiology and biochemistry, and its importance in relation to various aspects of hepatic and renal function has been given considerable …
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Ein Radioimmunoassay (RIA) für die Messung von Dehydroepiandrosteron (DHEA) im Speichel
… die Messung von DHEA im Serum stellen wir einen Radioimmunoassay für die Messung im Speichel vor. Der Testkit wurde in seiner Stabilität, Prezision und einfacher Handhabung validiert. Die Korrelation zwischen Serum und Speichel war hoch. Junge Probanden zeigten eine zirkadiane Rhythmik der …
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Preparation of Immunogens and Antisera Monitoring for Eventual Radioimmunoassay Development of Benzidine.
Benzidine-based dyes have been evaluated by the National Institute for Occupational Safety and Health and determined to be potential human carcinogens. Several studies show that benzidine-based dyes are associated with animal disease states and human bladder cancer.
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Radioimmunoassay of insulin and glucagon with studies on the obese hyperglycaemic syndrome in mice
Sensitive and precise radioimmunoassays for insulin and glucagon have been established. Although it was possible to employ similar precepts to the development of both hormone assays, the establishment of a reliable glucagon radioimmunoassay was complicated by the poor immunogenicity and instability …
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Determination of an Estrous Cycle in Female Giraffes (Giraffa Camelopardalis) By Radioimmunoassay of Fecal Hormones
… of progesterone and estradiol were determined by radioimmunoassay. The average cycle length for each female was determined by their inter-progesterone peak duration. Averages ranged from 10 to 14 days. There was a trend for male interest/breeding to occur one sample (approximately 2 days) before a …
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Applications of a radioimmunoassay technique to the study of luteinizing hormone secretion in the rat
A sensitive and reproducible double antibody radioimmunoassay technique, requiring 50ul of unknown serum or plasma per assay tube, is described for use with 125I and rabbit anti-rat LH serum. The assay system was applied to the study of LH secretion in rats under both normal and experimentally …
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Measurement of Maternal Serum Estrogen Levels in Normal and Complicated Pregnancies by Radioimmunoassay. Their Use in Evaluation of Fetal Well-Being.
The past fifteen years in this country have seen the perinatal period of human existence become the subject of increasing research. Perinatal mortality rates in the United States remain high by comparison with those observed in other developed countries of the world. This difference may be …
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Pigeon breeder's disease: the clinical spectrum and humoral response; an investigation of the nature and extent of extrinsic allergic alveolitis due to pigeon-derived antigens among pigeon fanciers continuing to pursue the pastime, with particular reference to early clinical and humoral-mediated responses following exposure
… were estimated by sensitive and quantitative radioimmunoassay techniques. Selected pigeon fanciers also underwent detailed pulmonary physiological assessments including monitoring of lung function after antigen challenge. In particular, immediate components of the condition both clinical and …
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Determination of reference intervals and decision limits for thyroid stimulating hormone and thyroxine on cord blood samples
… blood, as well as to compare the previous TSH radioimmunoassay with the current electrochemiluminescence immunoassay. Methods: Utilizing residual samples from the Peninsula Maternal and Neonatal Services (PMNS) CH Screening Program, this prospective study collected samples from uncomplicated …
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The effect of prenatal androgen exposure on sexual differentiation and postnatal growth in beef cattle
… analyzed for progesterone (P₄) concentration by radioimmunoassay and the P₄ profiles were used to estimate the age at puberty and estrous cycle lengths. At 4, 8 and 12 months of age blood samples were collected every 15 min for 12 h from three male, female and androgenized female calves. Serum …
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Studies into the release mechanisms of human placental lactogen in vitro
… incubation media was measured with a specific radioimmunoassay. Particular features of the assay were the use of high concentrations of reagents, and the separation of antibody-bound and free hormone by ethanol fractionation. The assay had a useful range of 75-800 ng HPL/m1 with interassay and …
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Bestimmung von "Brain Natriuretic Peptide"(BNP) bei gesunden Hunden
… Messung von Plasma BNP wurde ein kommerzieller Radioimmunoassay verwendet. Eine Validierung des Testkits mit der Untersuchung von Präzision, Richtigkeit, Sensitivität, Spezifität und Stabilität wurde zuvor durchgeführt. Als Studienteilnehmer dienten 79 gesunde Hunde (41 Rüden, 38 Hündinnen) im …
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Behavioral and Reproductive Aspects of Captive Maxwell's Duiker (<em>Cephalophus mawellii</em>) Husbandry
… to determine levels of progesterone through radioimmunoassay (RIA). Fluctuations in progesterone levels revealed no clear estrous cycles. Animals showed less variability in progesterone levels and more concordance among animals once introduced into group housing. Identifying activity budgets …
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Mechanisms controlling in vitro progesterone synthesis by the ovine placenta
… blood samples were analyzed for progesterone by radioimmunoassay. Immediately after each ewe was sacrificed, cotyledons were collected from different areas of each placenta, minced, and incubated in 2 ml of culture medium for either .5, 1, or 2 h. Minced tissue (100 mg) was incubated either in …
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The acute effects of transdermal testosterone precursor administration on serum steroid hormone levels in females
… total testosterone levels were analyzed using radioimmunoassay. Although the trend seemed to indicate that serum levels did not rise after application, several subjects had physiologically impossible values, which appear to be due to methodological errors. That information, along with the …
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Central Cardiovascular Effects of Arginine Vasopressin in the Lateral Habenular Region in Anesthetized Rats
Recent radioimmunoassay and immunohistochemical studies have shown the presence of vasopressinergic and oxytocinergic fibers extending from the paraventricular and suprachiasmatic nuclei in the hypothalamus to other brain structures. The lateral habenular region in the rat brain has been shown to …
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Maintenance of pregnancy in goats: Relationships between the fetus, the corpora lutea and the concentrations of progesterone in the maternal plasma
… concentration of progesterone was measured by radioimmunoassay of plasma samples collected frequently from the jugular vein from day 0 to day 75.
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Monosialylgangliosides from human meconium: characterization using specific anti-oligosaccharide antibodies
… anti-oligosaccharide sera in a direct-binding radioimmunoassay using nitrocellulose filters to collect immune-complexes. Radiolabeled oligosaccharide alditols which were recognized by specific antisera were affinity purified by eluting nitrocelIulose filters containing antibody-oligosaccharide …
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Possible thermoregulatory contributions of plasma B-endorphin during prolonged exercise in humans
… and hot/dehydration (HD). Piβ-EN was measured by radioimmunoassay at -30 min, 0 min, and at 15 min intervals thereafter. Change in plasma volume (ΔPV) was measured to determine its effect on Piβ-EN concentration. Preexercise Piβ-EN levels averaged 23.7 ± 2.6 pg•ml⁻¹ in all conditions. The greatest …
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