Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"Rad53"”.
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S-phase checkpoint activity and function throughout the cell cycle
… that the S-phase checkpoint effector kinase Rad53 phosphorylates its targets to inhibit origin firing outside of S-phase upon DNA damage when there is no replication. I then set out to test whether inhibition of origin firing by Rad53 outside of S-phase might be important for faithful DNA …
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Elucidation and characterisation of checkpoint kinase interactions with the replisome
… of the budding yeast checkpoint kinase Rad53 with the replisome. Using this technique, Pol1, a polymerase involved in DNA replication, was found to be a putative Rad53 interactor. Pol1 interacts with the FHA1 domain of Rad53 and abrogation of this interaction leads to loss of viability …
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HISTONE-DEPENDENT AND HISTONE-INDEPENDENT PATHWAYS FOR RAD9 CHROMATIN RECRUITMENT AND CHECKPOINT ACTIVATION
… and activation of the effector checkpoint kinase Rad53. This is a key step in the signal transduction cascade; it can be easily visualized as a hyperphosphorylated slower-mobility form by Western blotting and it is generally used as a marker to monitor checkpoint activation. Recent work …
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Coordination of origin licensing and cell cycle entry
… by a checkpoint that is dependent on the Rad53 checkpoint kinase, but is distinct from other cellular surveillance mechanisms that Rad53 is involved in. I have gained further understanding of the control of origin usage through a genetic approach that identified novel factors that enhance …
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Investigating the mechanisms of action of the SNF2-homolog protein Fun30
… of the cell cycle. Under these conditions Rad53 was found to be phosphorylated which is consistent with the activation of the S-phase checkpoint. In order to investigate the role of Fun30 in DNA replication samples were prepared to map the genome-wide locations of Fun30 at different stages …
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Saccharomyces cerevisiae DNA helicases Mph1, Srs2 and Sgs1 collaborate for the reinitiation of stalled or collapsed replication forks
… homologen DNA-Duplexen. Deletion der Gene MEC1, RAD53, RAD9, RAD17 und RAD24, die an der Checkpoint-Antwort auf DNA-Schäden beteiligt sind, kann ebenfalls zu einer Aufhebung des synthetischen Defektes führen. Darüber hinaus ist die rad54-Deletion sub-additiv zu mph1 in Bezug auf einen spontanen …
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Regulation of Histone Covalent Modifications During Yeast Apoptosis
… forkhead homology-associated domain 1 (FHA1) of Rad53 and H2BS10ph was uncovered. This interaction inactivates the DNA damage checkpoint pathway and promotes apoptotic chromatin condensation. Thus, both mechanisms may contribute to chromatin remodeling event that govern apoptotic chromatin …
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Identification and characterization of novel genes involved in DNA double strand break repair process in the yeast Saccharomyces cerevisiae
… through interaction with checkpoint kinases Rad53 and Chk1. We also propose a novel role for yeast uncharacterized ORF HUR1, in NHEJ pathway through its association with Nej1. We also report that BUB1/BUB2 may affect the efficiency of NHEJ by limiting cell cycle progression. We suggest their …
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ATR SIGNALING IN GENOME REPLICATION AND MAINTENANCE
… action in activating the downstream kinase Rad53. I also found evidence supporting that in humans, ATR can also function in a replication-correlated manner. Overall, we proposed that Mec1/ATR performs key functions during ongoing DNA synthesis to prevent genomic instability that are largely …
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From the End to the Middle: Regulation of Telomere Length and Kinetochore Assembly by the RNR Inhibitor SML1
… damage. In response to various forms of damage, Rad53 and Dun1 are activated and then phosphorylate numerous downstream targets, including the Rnr1 inhibitor Sml1. In this study, it was shown that the phosphorylation of Sml1 triggers its ubiquitylation by the Rad6-Ubr2-Mub1 ubiquitin ligase …
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Control of Replication Fork Stability by Chromatin and S-phase Checkpoint
… experimentos de re-expresión controlada de Rad53 junto a diversos candidatos que revelaron la implicación de Rad51, Mus81 y Rad5. Además, observamos cómo la sobreexpresión de Mre11 suprime esta letalidad. El análisis de incorporación de BrdU nos ha permitido demostrar que dicha pérdida de …
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Etablierung und Anwendung von Hochdurchsatz-Verfahren zur Identifizierung strahleninduzierbarer Gene in der Hefe
… - Bestrahlung konnten zudem mit DUN1 und RAD53 zwei Gene als induzierbar eingestuft werden, die an der Signaltransduktion, die zur Induktion der RNR-Gene notwendig ist, beteiligt sind. Schließlich zeigte auch die stichprobenartige Überprüfung der mit Microarrays erzielten Expressionsdaten …