Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 61 for “"Rad51"”.
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Interrogation and engineering of RAD51:BRC repeat interactions
… the DSBs. Central to HR is a recombinase protein RAD51, which forms a nucleoprotein filament with the broken ends, and allows efficient homology search. RAD51 is a promising therapeutic target in oncology. BRCA2 is a crucial mediator of RAD51 function and interacts with RAD51 through a set of 8 …
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RAD51-MEDIATED ABASIC SITES PROTECTION PREVENTS REPLICATION FORK COLLAPSE.
… for the homologous recombination (HR) protein RAD51 in AP site protection and repair during genomic DNA replication. Our findings provide direct evidence that unrepaired AP sites generate discontinuous ssDNA stretches at replication forks onto which RAD51 binds preventing MRE11-RAD50 …
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Mechanisms of human RAD51 regulation by RAD52 and BRCA2
The RAD51 recombinase assembles as helical nucleoprotein filaments on single-stranded (ss)DNA substrates to mediate homologous DNA recombination (HR) and replication fork protection, processes vital in human cells for the maintenance of genome stability. RAD51 assembly is controlled by two key …
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Biochemical and towards structural characterization of RAD51 and RAD51AP1 complexes
… stability through the accurate repair of DSBs. RAD51 is the eukaryotic HRR recombinase that promotes HRR by assembling onto ssDNA to form nucleoprotein filaments and catalyses key HRR steps of strand invasion and homology search in the sister chromatid. A large number of accessory factors and …
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Analysis of the functions and interactions of RAD51 paralogues in Trypanosoma brucei
… and a key component of homologous recombination, RAD51, also plays a role in VSG switching. T. brucei encodes four proteins distantly related to RAD51; termed RAD51 paralogues, named RAD51-3, RAD51-4, RAD51-5 and RAD51-6. Two of these RAD51 paralogues, RAD51-3 and RAD51-5, have been shown to …
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Loss of rad51 in zebrafish (Danio rerio): a novel Fanconi anaemia model
RAD51 is an indispensable homologous recombination protein, necessary for strand invasion and crossing over. It has recently been designated as a Fanconi anaemia (FA) gene, following the discovery of two patients carrying dominant negative mutations. FA is a hereditary DNA repair disorder …
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Mechanism of regulation of human RAD51 recombinase through post translational modifications & mediator proteins
RAD51 protein plays an important role in homologous genetic recombination (HR), an essential DNA metabolic process used by cells to faithfully repair the most deleterious forms of DNA damage and maintain genomic integrity. RAD51 along with its bacterial counterpart RecA, bacteriophage UvsX and …
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Novel Structures of RAD51 Reveal Mechanisms in DNA Damage Repair and Genomic Stability
The RAD51 protein contributes to the maintenance of genomic stability by promoting the repair of DNA double-strand breaks and the protection of DNA replication forks. RAD51 functions alongside the tumour suppressor protein BRCA2 to catalyse DNA strand-exchange reactions which form an integral part …
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Characterization of the DMCI and Rad51 homologues and the process of meiosis in trichomonas vaginalis
… this study, we induced the expression ofDMCl and Rad51 homologues-key players in Homologous Recombination-using a system of tetracycline induction. We localized DMCl to both the cytoplasm and the nucleus, while Rad51 is localized to the nucleus. We performed a DNA strand exchange that suggests …
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The Rad51 family of proteins: Interactions, vitamin D, and implications in head and neck cancer
… repair. Five human proteins with homology to Rad51 known as the Rad51 paralogs, Rad51B, Rad51C, Rad51D, XRCC2, and XRCC3, whose loss of function in cell lines leads to high chromosomal instability. Previous studies have shown Rad51C participate in two paralog protein complexes, one containing …
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Vitamin D- induced down regulation of RAD51 in head and neck squamous cell carcinoma (HNSCC), In Vitro and In Vivo
… of VD<sub>3</sub>to hamsters downregulates Rad51 expression in the buccal pouch and hinders the onset of tumor formation. Rad51 is a protein that plays a critical role in cell proliferation and homologous recombinational DNA repair. In the in vitro model, we show that Rad51 expression …
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The behavior of RAD51D and XRCC2 in response to drug induced DNA damage and a continuing study of the fly RAD51 paralogs
… of this experiment followed two Drosophila Rad51 paralogs, dmRad51D and dmXRRC2, and using constructs tagged with GFP, found that they entered the nucleus in response to drug induced DNA damage. Approximately one hour after the induction of DNA damage via bleomycin, dmRad51D and dmXRCC2 …
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Characterisation of factors that regulate homologous recombination and antigenic variation in Trypanosoma brucei
… form of homologous recombination, as mutation of RAD51 and a related gene, RAD51-3, impairs the process. BRCA2 has emerged as a significant regulatory factor during RAD51-catalysed recombination. In humans, BRCA2 contains eight BRC repeats, six of which have been shown to bind RAD51. Similar …
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Caretaker-Gen-Syndrome
… Verbindung zwischen den FA-Proteinen und der RAD51-Familie. FA-Zellen aller bisher bekannter Komplementationsgruppen zeichnen sich durch Hypersensitivität gegenüber DNA-„interstrand crosslinks“ (ICLs) aus, zu deren Behebung u.a. die homologe Rekombinationsreparatur (HRR) eingesetzt wird, bei …
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Single molecule investigations into DNA replication, recombination and repair
… and conduct repair. Srs2 dismantles presynaptic Rad51 filaments and prevents its re-formation as an anti-recombinase. However, the molecular mechanism by which Srs2 accomplishes these tasks remains unclear. Here we report a single-molecule fluorescence study of the dynamics of Rad51 filament …
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Dynamics of DNA-repair factors and chromosomes studied by laser-UVA-microirradiation and laser-photobleaching
… Laser-UVA-microirradiation The protein Rad51 is essential for the repair of double-strand breaks (DSBs) via the conservative homologous recombination repair pathway. To test the hypothesis that Rad51 localizes to damaged sites during DSB repair, a laser-UVA-microirradiation system was …
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Investigation of The Roles of Asf1 and Caf-1-Mediated Chromatin Assembly In The Human Dna Damage Response
… ASF1 binding to histones, hinders loading of Rad51 onto ssDNA during homologous recombination, as a consequence of reduced recruitment of the Rad51 loader MMS22L/TONSL to ssDNA, resulting in persistent RPA foci, extensive DNA end-resection, and persistent activation of the ATR-Chk1 pathway. By …
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Cell-Cell Junction Signaling Regulating DNA Double-Strand Break Repair In Breast Cells
… foci formation kinetics for the DSBR proteins RAD51, MRE11, and γ-H2AX in single mammary epithelial cells. RAD51 is required for most HR, whereas MRE11 and γ-H2AX function in HR as well as DNA damage signaling. Interestingly, ECM signaling up-regulates HR in cells that have “correct” in …
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The role of TvTrxR in drug resistance and characterization of TvRad51 in homologous recombination in Trichomonas vaginalis
… in Drug Resistance and Characterization of TvRad51 in Homologous Recombination in Trichomonas vaginalis Abstract By Melissa Hopper University of the Pacific 2016 In recent years, prevalence of metronidazole-resistant cases of Trichomonas vaginalis has been on the rise. With nearly 10% of …
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Regulación de la rutas de tolerancia a daños replicativos dependientes de RAD6, RAD5 y RAD52 durante el ciclo celular en Saccharomyces cerevisiae
… se demuestra que las proteínas de HR Rad52, Rad51 y Rad57 colaboran con la maquinaria de TLS (ubiquitinación de PCNA mediada por Rad6/Rad18 y las polimerasas Rev1/Pol z) para reparar las lesiones de ssDNA a través de una función no recombinogénica, y en consecuencia son necesarias para la …
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