Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 10 of 10 for “"Rac activation"”.
-
Rapid Rac GTP-ASE activation in live cells by mechanical stress is independent of Src
… molecule integrins. The temporal and spatial activation of Rac, a small guanosine triphosphatase, was quantified using a fluorescent resonance energy transfer (FRET) method that measures changes in Rac activity in response to mechanical stresses by quantifying intensity ratios of ECFP …
-
HIV-1 Envelope-Induced Signaling Mediates Actin Cytoskeleton Rearrangements Necessary for Fusion and Entry
… binds to the primary receptor CD4. This interaction promotes actin cytoskeletal rearrangements in the target membrane that bring the chemokine coreceptor, CCR5 or CXCR4, into close proximity for binding and induces conformational changes in gp120 that allow it to couple to the coreceptor. …
-
Regulation of Homophilic and Heterophilic Adhesion Among Classical Cadherins
… of filamentous actin correlated with the Rac activation amplitudes in E-cadherin expressing cells. The results also showed that lateral cadherin clustering, which is thought to be an initial response to cadherin ligation, occurs in E-cadherin expressing cells as a result of both …
-
Upregulation of PIP3-dependent RAC Exchanger 1 (P-Rex1) Promotes Prostate Cancer Metastasis
Activation of Rac by guanine-nucleotide exchange factors (GEFs) at the leading edge of the cell plays an important role in directional cell migration, a critical step of tumor metastasis cascades. We investigated the role of P-Rex1, a novel specific GEF for Rac, in human prostate cancer metastasis. …
-
Ethanol Impairs Mechanisms of Macrophage Phagocytosis and Cytokine Production
… RAW264.7 cells exposed to NSC23766 (a Rac1 inhibitor) show 45% suppression in actin polymerization to the phagosome, regardless of ethanol exposure. This underscores the importance of normal Rac activity during FcgammaR-mediated phagocytosis. Following 15 minutes of IgG-coated bead …
-
Molecular mechanisms of signal transduction in neutrophil chemotaxis
… actin cytoskeleton reorganization and interaction between the cells and the substratum on which cells migrate are still not fully understood. In my first research project, we have identified a signaling pathway, mediated by non-receptor tyrosine kinase Lyn that is essential for localized …
-
Identification of the Role of P-Rex1 and its Adaptor Functions in Neutrophils
P-Rex1 is a guanine-nucleotide exchange factor (Rac-GEF) for the small GTPase Rac, recognised for its involvement in neutrophil migration and ROS generation in response to GPCR activation—functions traditionally attributed to its Rac-GEF activity. Additionally, previous findings from our laboratory …
-
Function and Regulation of the Cell Fate Determinant Numb in Polarized Epithelial Cells
… transition (EMT). In Numb-depleted cells, Rac1-GTP loading is enhanced, which corresponds with increased rate in loss of cell-cell adhesion and increased lamellipodia formation, following depletion of extracellular calcium and HGF stimulation, respectively. Together, this work identifies a …
-
Dock180/Elmo proteins are critical regulators of phagocytosis,macropinocytosis and chemotaxis in Dictyostelium
NOTE: THE SPECIAL CHARACTERS IN THIS ABSTRACT CANNOT BE DISPLAYED CORRECTLY ON THIS PAGE. PLEASE REFER TO ABSTRACT IN THE ATTACHED FILE FOR AN ACCURATE DISPLAY. In this study, we report the characterization of Dock/Elmo proteins, a novel family of RacGEF's in Dictyostelium discoideum and describe …
-
Analysis of signal transduction pathways and the cytoskeleton in VASP-deficient cell lines and mouse models
… (Evl). VASP was initially discovered and characterized as a substrate for cGMP- and cAMP-dependent protein kinases (cGKs and cAKs). Ena/VASP proteins are involved in Actin-filament formation, plasma membrane protrusion, acceleration of Actin-based motility of Listeria and the establishment of …