Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 18 of 18 for “"Rab7"”.
-
Characterization of the entry mechanisms utilized by the alphavirus venezuelan equine encephalitis virus to infect mosquito cells
… and characterized in the mosquito cell. Rab5, Rab7 and dynamin are shown to be involved and necessary for VEEV entry and infection in mosquito cells. A novel entry assay is used to confirm that VEEV requires a low pH to enter mosquito cells. This work represents the first characterization of …
-
Mechanism and Functions of STING-Induced Noncanonical Autophagy
… between V-ATPase and the ATG16L1-WD40 domain on Rab7-coated endolysosomal compartments. Additionally, we unveiled that STING-induced noncanonical autophagy leads to the activation of MiT/TFE transcription factors (TFEB, TFE3, and MITF), promoting endolysosomal biogenesis. Furthermore, we showed …
-
Ubiquitylation regulates vesicle trafficking and innate immune responses on the phagosome of inflammatory macrophages
… ubiquitin chains with cytosolic proteins such as Rab7. Rab7 a major regulator of vesicle trafficking could be shown to be ubiquitylated on phagosomes. I further showed that phagosomal recruitment of the E3 ligase RNF115 is enhanced upon IFN-γ stimulation and RNF115 is responsible for most of the …
-
The Role of Vps54 in Drosophila melanogaster Neuronal Development and Age Progressive Neurodegeneration
… that Vps54 may be interacting with small GTPases Rab7 and Rab11 at different life stages to further regulate motor neuron development and function. Taken together, these data suggest that Vps54 plays a major role in the development and functional regulation of motor neurons, while additionally …
-
PROTEINi screens identify ORP9 as a novel regulator of KRAS-driven signalling
… and TAT-HPX caused accumulation of PI4P on Rab7-positive endosomes, suggesting inhibition of ORP9-mediated lipid transfer. ORP9 knockdown reduced MEK1/2 phosphorylation, ERK1/2 phosphorylation and GFP expression downstream of mutant KRAS, but not mutant BRAF, highlighting ORP9 as a regulator …
-
"Molecular Analysis of Virulence Factors Unique to Leptopilina heterotoma-a Parasitic Wasp of Drosophila spp."
… marker signals of the late endosomes, marked by Rab7-GFP; higher VLP signals correlated with late endosomes in plasmatocytes for animals that underwent a longer post-infection recovery time. Some colocalization of VLP was also observed with the Rab5-GFP as well.</p>
-
Structural Functional Investigation of HAP40
… morphology. Loss of BΦ selectively impaired Rab7+ endosome growth and disrupted HTT targeting to endosomal membranes.</p> <p>Furthermore, in a Drosophila model of HD, co-expression of mutant HTT with HAP40-ΔBΦ exacerbated neurodegeneration relative to full-length HAP40, indicating that the BΦ …
-
ATG8 Regulation and Function in Non-Canonical Autophagy
… to canonical autophagy, others such as the Rab7 GAP, TBC1D5, appear common to both pathways. Also presented within this study are potentially novel ATG8 interactors that may, upon further investigation, shed light on the unknown role of ATG8s during non-canonical autophagy.
-
The Mechanisms of Tau Entry to the Cytosol
… clathrin-dependent manner with late endosomal Rab7 GTPase involvement. In contrast, entry to primary and human neurons was via a clathrin- and dynamin-independent route that was sensitive to membrane cholesterol levels. Extraction of cholesterol from membranes rendered neurons extremely …
-
Proteomic analysis of the sorting machineries involved in vesicular traffic between the biosynthetic and endosomal compartments
… selectively recruited together with Rab5 and Rab7. Thus, the proteomic analysis of AP-1A and AP-3 coated structures suggest that both AP coats use similar principles - coats, actin nucleation devices and Rab fusion machineries - to assemble supramolecular structures needed for membrane …
-
Investigations into the Nature of the Endosomal System in Plasmodium falciparum
… the retromer cargo-selective complex and Rab7, were found on a previously un-described inherited structure adjacent to the parasite Golgi apparatus and in close opposition to nascent rhoptries (specialized secretory organelles required for invasion). The retromer cargo-selective complex …
-
Deciphering The Role of Huntingtin In Endosomal Functions
… through the endosomal-lysosomal pathway (via Rab7). Disturbances in normal Rab functions could lead to abnormal cargo recycling or degradation, potentially contributing to neurodegeneration. HTT has been shown to interact with Rab5 on early endosomes, which direct cargo traffic towards …
-
Analysis of the maturation of Rhodococcus equi-containing vacuoles in macrophages
… acquired some late endosomal markers, such as Rab7, LAMP-1, and Lamp-2, they did not acquired vATPase, did not interact with pre-labeled lysosomes, and failed to acidify. These data clearly suggest that the RCV is a compartment which has left vacuoles that resemble multivesicular body …
-
Anaplasma phagocytophilum remodels its host cell-derived vacuole into a protective niche by redecorating the vacuolar membrane with select Rab GTPases and bacterial proteins
… and acquisition of the late endosomal marker, Rab7, and lysosomal marker, LAMP-1, implicating bacterial-derived proteins in the ApV's altered fusogenicity. Therefore, we rationalized that A. phagocytophilum-encoded proteins that associate with the AVM may establish interactions with the host …
-
The Dynamics and Function of the Endolysosomal/Lysosomal System
… membrane was also shown to be marked by Rab7, Rab9, PI(3,5)P2 supporting the role of endolysosomes a highly active and dynamic principal site of hydrolase activity. The contributions of VAMP7 and VAMP8 to endolysosome fusion, measured by delivery of endocytosed cargo from late endosomes …
-
Rab-domain dynamics in endocytic membrane trafficking
… coordinated with the concomitant acquisition of Rab7, suggesting "Rab conversion" as mechanism of transport between early- and late endosomes.Altogether, this thesis research has shown that first, Rab-machineries can be acquired and lost from membranes. Second, such dynamics provide a …
-
Differential expression of proteins involved in sEV biogenesis and secretion in Parkinson’s disease and Dementia with Lewy bodies
… including key proteins such as P62, Atg7, Rab7 and LAMP2. This suggests that although sEV proteins are affected, this may not necessarily influence degradation pathways such as the ALP in the model presented in this thesis. One striking change observed in this thesis was a significant …
-
Cellular Prion Protein (PrPC): Identification and Characterization of Novel Interacting Partners
… novel interacting partners of the GTPase family (Rab7a and Arf1) which have a suggested role in vesicle trafficking as well as the cytoskeleton associated protein alpha-tubulin 1 were further investigated using confocal laser scanning microscopy and reverse co-immunoprecipitation. Both reverse …