Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"Rab11"”.
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Characterization of Fluorescent biosensor for Rab11 GTPase
L'abstract è presente nell'allegato / the abstract is in the attachment
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Rab11 Regulates Lumen Formation and Morphogenesis in the Developing Pancreas
… decade of studies. Here, I show that deletion of Rab11A and Rab11B (Rab11) in the developing pancreatic epithelium (Rab11pancDKO) disrupts both of these processes. The loss of Rab11 in the pancreas results in ~50% neonatal lethality, with the surviving pups failing to thrive. Surviving adult …
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Analysis of the role of rab11-FIP3 phosphorylation during cytokinesis
… is at the heart of mammalian cytokinesis. Rab11-FIP3 (also referred to as FIP3) in complex with Rab11 plays a key role in the delivery and targeting of recycling endosomes to the furrow; this is essential for completion of cytokinesis. FIP3 undergoes spatial and temporal dynamics during …
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Beach1 functionally antagonizes Rab11 during development and in regulating synaptic morphology
BEACH proteins comprise an evolutionarily conserved family characterized by the presence of a BEACH (Beige and Chediak-Higashi) domain of unknown function. They have been shown to play a role in a number of important cellular processes, ranging from cytokinesis to synaptic transmission, and …
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An investigation of the functional role of GRAB in the context of Rab3, Rab8 and Rab11
… is one such protein which is an effector for Rab11a and a Guanine nucleotide Exchange Factor (GEF) for Rab8a. Rabin8 participates in a conserved Rab activation cascade which is critical in the formation of primary cilia. Data presented in this thesis has shown that GRAB interacts with Rab3a, …
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Engineering Optogenetic Control of Endocytic Recycling: Controlling Rab11 Function in Drosophila melanogaster using Engineered Light-Responsive Nanobodies
… One member of this large family of proteins is Rab11. Rab11 is responsible for endosome recycling: returning membrane proteins and receptors from intracellular recycling endosomes to the cell membrane, where these membrane proteins and receptors may be reused. Although the exact mechanism of …
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Structural Investigations of Nematode Anhydrobiotic and Rab11-FIP Proteins by Far-UV Circular Dichroism and Mid-IR Fourier Transform Infra-Red Spectroscopy
… the functionality of the human homolog.[1] The Rab11-FIP3 homo-dimer predicted coiled coil protein fragment was confirmed by CD spectroscopy and TFE titration. For the Rab11-FIP2 homo-dimer, mutation of the valine residue 456 and the leucine residue 457 to glycine residues was found to …
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A Role for the Phosphoinositide Lipid Kinase PI4KIIIbeta in Breast Oncogenesis and Akt Activation
… generation. The PI4KIIIβ interacting protein, Rab11, is likely involved in PI4KIIIβ mediated Akt activation, as RNAi-mediated depletion of Rab11 suppressed the effect of PI4KIIIβ overexpression on Akt activation. Furthermore, PI4KIIIβ overexpression altered cellular Rab11 distribution and led …
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A functional analysis of Rab11a and Rab11b in endosomal recycling
Rab11 GTPases are a family of three monomeric G proteins which are involved in a number of cellular processes including cell division, cell migration and cargo trafficking. Rab11a and Rab11b share over 89% sequence similarity, while Rab25 is approximately 60% similar to Rab11a and Rab11b. Both …
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Rab-domain dynamics in endocytic membrane trafficking
… Total Internal Reflection (TIRF) microscopy, Rab11-, but neither Rab4- nor Rab5-positive vesicles were observed to fuse with the plasma membrane. Rab4-positive membranes, however, could be induced to fuse in presence of Brefeldin A. Thus, these experiments complete the view of the recycling …
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Promoting Central Nervous System Regeneration by Targeting Intracellular Pathways
… Phosphorylated protrudin preferentially binds Rab11, a small GTPase involved in axon transport of growth receptors. This association is required for neurite outgrowth and for anterograde movement of this complex. We found that endogenous protrudin is excluded from mature axons similar to other …
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Differential Selection Pressure Among Duplicated Genes In Teleosts
… material subject to selection. Using myo5, rab11, and rab27 gene families as models, I examined the evolutionary rate differences among duplicated genes and whether selective forces (e.g. purifying selection or positive selection) could be identified in one or both duplicated gene clades. I …
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On connections between metazoan cellular metabolism and cell size
… of the recycling endosome regulating protein RAB11 was identified to be at least partially responsible for the cell size regulation by the mevalonate pathway. Furthermore, the link from the mevalonate pathway to RAB11 was found to regulate basal autophagic flux, thus providing a novel …
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FUNCTIONAL CHARACTERIZATION OF DENND5A/RAB6IP1 IN EARLY EMBRYONIC DEVELOPMENT
… interaction partners have been discovered, Rab6, Rab11 and Sorting Nexin 1, which primarily locate Dennd5a to intracellular transport pathway. In Dr. Habas lab, Dennd5a was identified as a Daam1 interacting protein in a yeast two hybrid screen. Daam1 is a key player in non-canonical Wnt signaling …
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The Role of Vps54 in Drosophila melanogaster Neuronal Development and Age Progressive Neurodegeneration
… may be interacting with small GTPases Rab7 and Rab11 at different life stages to further regulate motor neuron development and function. Taken together, these data suggest that Vps54 plays a major role in the development and functional regulation of motor neurons, while additionally interacting …
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Proteomic analysis of the sorting machineries involved in vesicular traffic between the biosynthetic and endosomal compartments
… as well as members of a Rab machinery (Rab11, Rab14). This finding was further supported by in vivo colocalization studies of the AP-1A cargo CI-MPR with CYFIP2, a protein of the Wave/Scar complex, and the localization of Big2 and Git1 on Rab11 positive membranes (Matafora et al., 2001; …
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Rab35 Centered Membrane Trafficking Pathway Directs Apical Constriction During Drosophila Gastrulation
… Sbf/Rab35/CME directs the plasma membrane to Rab11 endosomes through a dynamic interaction with Rab5 endosomes. Finally, we find plasma membrane lipid cues in the form of PtdIns(3,4,5)P3 are a critical determinant for Sbf/Rab35 ratcheting engagement. These results suggest that periodic …
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The Role of Membrane Domains In Protein and Lipid Sorting During Endocytic Traffic
… classical recycling pathways defined by Rab4 and Rab11, but instead represents a novel route for PM recycling of raft-preferring cargo from late endosomes. I implicate Rab3 as a central regulator of this pathway and show that the Rab3 family is essential for PM homeostasis, as abrogation of all …
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The role of cell division during tissue morphogenesis
… KV rosette, where it acts as a landmark for Rab11-mediated vesicle trafficking to bring polarity components such as CFTR to the site of future lumen formation. Next, the early zebrafish embryo is used as a model to determine how the spindle is oriented to create a monolayer grid formation …
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The molecular mechanism of Dual leucine zipper kinase protein localization in neuronal stress signaling.
… suppression of Wnd protein levels. Moreover, Rab11 plays a critical role in regulating Wnd protein turnover to inhibit excessive neuronal loss. Finally, Chapter 3 discusses and addresses future directions, focusing on the study of palmitoylation-defective Wnd mutants, wild-type Wnd axon …
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