Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"Rab-GTPase"”.
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The regulation of yeast homotypic vacuole fusion by phosphatidic acid and diacylglycerol
… with each other via interactions between the Rab GTPase Ypt7 and its effector HOPS complex. Once tethered, compartments are pulled into tight contact with each other through the formation of trans-SNARE complexes during a docking stage. The final fusion stage proceeds through a hemifusion …
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Assessment of endosomes as sites of signal transduction in plants during bacterial attack
… the localisation of three members of the PRA1 RAB GTPase regulatory family of proteins. Furthermore this data was able to elucidate the differences in RFPRABF2b/ARA7 and RABF1/ARA6-RFP labelled LE/MVBs and their interaction with the TGN. Assessment of endosomal proteomes after flagellin …
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Biophysical characterization of KO513, a protein expressed in human invasive glioblastoma
… interacting partners includes members of the Rab GTPase family, membrane proteins, transcription factors and neurotransmitters. The overall in silico analysis suggest that K0513 may possess nucleotide exchange factor (NEF) activity for Rab3a. Recombinant proteins, non-codon harmonized (K0513W) …
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Genetic Analysis of Grinder Formation in Caenorhabditis Elegans: Regulation by RAB-6.2 and Its GTPase Activating Protein EAT-17
… I found that eat-17 encodes an ~825 amino acid Rab GTPase activating protein with a series of C-terminal coiled-coil domains. Its closest human homolog is Evi5, a putative oncogene whose function is not currently understood. Rabs are key regulators of vesicle transport, and cycle between active, …
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Exploring The Impact of Decreased Ampk Pathway Activity On Brain Injury Outcome
… Akt Substrate of 160 kDa (AS160), a Rab GTPase activating protein that controls Glut4 translocation. Additionally, AMPK negatively regulates energy-consumption by inhibiting protein synthesis via the mechanistic Target of Rapamycin (mTOR) pathway.</p> <p>Given that metabolic …
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Untersuchungen zur anterograden Bewegung und Vererbung von Mitochondrien in Saccharomyces cerevisiae
… die am Transport der Mitochondrien beteiligte Rab-GTPase Ypt11 konnten durch Expression dieser Mitochondrien-spezifischen Variante von Myo2 aufgehoben werden. Mittels Immuno-Elektronenmikroskopie gelang es, Myo2 auf der Oberfläche von isolierten Mitochondrien nachzuweisen. Diese Ergebnisse …
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The effects of the Parkinson’s disease-associated proteins LRRK2 and TMEM175 on the lysosomal profile of cultured mammalian cells under lysosomal stress conditions
… causing increased phosphorylation of its Rab GTPase substrates. This study shows that A549 cells with PD-associated Y1699C LRRK2 mutation were more abundant in phosphorylated Rab10 compared to WT cells. Currently, LRRK2 is considered the major kinase confirmed to phosphorylate Rab10 at …
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The Structural and Biochemical Characterization of Salmonella Host Specificity Determinant Gifsy-2 Gene E
… of the Salmonella-containing vacuole by cleaving Rab GTPases, Rab29, Rab32, and Rab38, thereby preventing the delivery of antimicrobial products to the vacuole. In order to gain an understanding of GtgE's proteolytic mechanism, we determined the structure of GtgE to 1.65Å using single-wavelength …
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Regulation of the cGAS-STING Pathway: Non-Agonist Activation and Cellular Trafficking
… trans-Golgi coiled-coil protein GCC2 and Rab GTPase Rab14 as key regulators of STING Golgi-exit. Moreover, I found increased autoantibodies production in Gcc2-/- mice in a STING-dependent manner. As another non-agonist approach to activate the cGAS-STING pathway, I found that Gcc2KO and …
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Klonierung und funktionelle Analyse des Aktinreorganisators p150-Spir
… signalübertragender Effektor, der den klei-nen GTPasen der Rho–Familie Rac und Cdc42 nachgeschaltet ist. Rho-GTPasen spielen eine Schlüsselrolle in der Regulation von zellulären Aktinstrukturen und steuern Prozesse in der Zelle, die Änderungen der Aktinstruktur erfordern, wie z.B. Änderungen der …
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An investigation of the functional role of GRAB in the context of Rab3, Rab8 and Rab11
Rab GTPases are the largest family of the Ras superfamily and are key regulators of membrane trafficking within the cell. There are over 60 members of the Rab family which localise to specific membrane compartments and interact with effector proteins to regulate membrane trafficking processes, such …
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Analysis of Hepatocyte Secretion Pathways: A Case Study on Hepatic Apolipoproteins, Serum Albumin, and Hepatitis C Virus
… I begin by describing a dominant negative (DN) Rab GTPase screen that I performed to identify Rab proteins involved in ApoE, ApoB100 or albumin secretion. The small Rab GTPases control individual steps of vesicular transport. I analyzed how expression of individual dominant negative Rab proteins …
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The regulation of yeast homotypic vacuole fusion and membrane trafficking events by sphingolipids, ergosterol, and lipid rafts
… fusion requires the activity of SNAREs, small GTPases, chaperones, tethering complexes, and specific regulatory lipids. This process occurs in four experimentally defined steps: priming, tethering, docking, and fusion. Work with baker’s yeast identified interdependence between a set of …
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Characterisation of the role of AMP-activated protein kinase in 3T3-L1 adipocytes
… fatty acid and cholesterol synthesis. The Rab GTPase activating proteins Akt substrate of 160 kDa (AS160) and tre-2/USP6, BUB2, cdc16 domain family member 1 (TBC1C1) have been identified as potential targets of both protein kinase B (PKB, also known as Akt) and AMPK which mediate glucose …
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Rab-domain dynamics in endocytic membrane trafficking
… molecular understanding of the endocytic system. Rab-GTPases have emerged as architectural key element thereof: Individual family members localize selectively to endosomal compartments, where they recruit a multitude of cytoplasmic effector proteins and coordinate them into membrane sub-domains. …
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Evolution of vesicular transport in kinetoplastids: dynamics and novel gene products
… three central paralogous trafficking families: Rabs, SNAREs and Rab-GAPs, which have defined roles in specific trafficking events. Further, proteomics was used to analyse a representative SNARE complex to explore compositional conservation between kinetoplastids and Opistokhonts. Overall there …
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The regulation of yeast homotypic membrane fusion by class C ABC transporters
… Sec17p (a-SNAP). Tethering requires Ypt7p (a Rab GTPase) and the HOPS effecter complex. SNARE complexes, including one R SNARE from a donor vacuole and three Q SNAREs from the acceptor vacuole, are formed in trans during docking of vacuoles. The membranes of the docked vacuoles are drawn …
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RabAnalyser: a computational framework to explore the cell-to-cell variability of Rab GTPases organization
L'abstract è presente nell'allegato / the abstract is in the attachment
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Mechanisms of Multivesicular Body Biogenesis and Exosome Release
… ILV der MVE für Bildung von Exosomen gefunden.Rab Guanosin triphosphatasen (GTPases) sind Regulatoren des intrazellulären Membransortierung. Um die Wirkung der GAPs (GTPase activating proteins) auf Exosomenfreizetzung zu untersuchen, wurde ein Screen eingesetzt. Die Expression von TBC1D10B, …