Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 202 for “"RT-qPCR"”.
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Human Norovirus in Artificial and Environmental Marine Water: Development of Antibody Based Rapid Methods
… compares commonly used molecular techniques (RT-PCR) and a commercial enzyme immunoassay for the detection of NoV. One oyster sample was positive for norovirus GII at 3.5 ± 0.2 log10 genomic equivalent copies/g digestive tissues, however the surrounding water tested negative for NoV. Zeolite …
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Evolution-dependent SARS-CoV-2 vaccination success determined by neutralizing antibodies and reliability of point-of-care testing
… and to estimate vaccination success. Since RT-qPCR tests were not sufficient for mass testing, point-of-care tests (POCTs) for antigen detection became necessary. In 2020, we determined the sensitivity of a newly developed POCT by comparison with RT-qPCR and virus cultivation assays. The …
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Quantitative analysis of individual flue-cured tobacco seed tissues reveals Tobacco mosaic virus infection in embryos
… cDNA for analysis. A quantitative real-time PCR (RT-qPCR) assay was developed to quantify viral titers in each component, while endpoint PCR confirmed RT- qPCR results and established a threshold viral cycle (Ct) value. Endpoint PCR results revealed viral accumulation in all three components of a …
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Immunomodulation of commercial line and Piau Brazilian Naturalized breed pigs in response to vaccination against Mycoplasma hyopneumoniae and Pasteurella multocida type D
… para os patógenos em estudo para análise de RT- qPCR. Às dez semanas de idade, os baços dos animais foram coletados para ensaio de proliferação celular e, amostras de lavado broncoalveolar (BALF) foram coletadas para mensurar a produção de óxido nítrico e RT-qPCR. RNA foi extraído de células …
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Evaluation of Anti-cancer effect of a Novel Formulation from Umbilical Cord Blood Stem CellDerived Exosomes and Retinoic acid as a potential therapeutic candidate against Malignant Melanoma
… identified as 10 µM for RA and 120 µl of 107 particles/ml for CBSC-derived exosomes. Both doses significantly exerted cytotoxic and genotoxic effects on FM55 and CHL-1 melanoma cells compared to lymphocytes from healthy and melanoma patients where both treatments showed genoprotective effect …
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Creating CRISPR-Cas9 genome edited iPSC lines to model a patient-specific mutation in mitochondrial disease
… energy expenditure like the nervous system, heart, and kidneys. However, disease mechanisms for the heterogeneous group of diseases have not yet been fully elucidated. Harnessing CRISPR-Cas9 genome editing in induced pluripotent stem cells (iPSC) provides an opportunity to model mt-aaRS …
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Tracking Tobacco Mosaic Virus Infection from Infected Seeds to Seedlings Confirms Seed Transmission in Tobacco (Nicotiana tabacum L.)
… germination. Total RNA was extracted from each part and synthesized into cDNA for analysis. A quantitative real-time PCR (RT-qPCR) assay was used to determine TMV concentration of each sample. Endpoint RT-PCR was used to determine a conservative threshold value from the RT-qPCR results. These …
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Implementation of global miRNA biomarker discovery in human plasma
… improved patient stratification needed to support further implementation of precision medicine will require developments in molecular diagnostics and identification of novel biomarkers. Small non-coding RNAs, specifically miRNAs, have been shown to exist extracellularly and are stable in …
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Investigation of Endogenous Retroviruses in the Pathogenesis of Sporadic Amyotrophic Lateral Sclerosis (ALS) by
… While HERV-K (HML-2) transcripts were reported as elevated in premotor cortex samples from an ALS American cohort by Li et.al. (2015) the RT-qPCR assays performed in this sudy, using the same primers and reaction conditions in a larger ALS UK cohort were unable to corroborate these …
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Thyroid hormone action and disruption in the brain: Xenopus as a model to study disruption of thyroid hormone availability on early brain development
… are essential for normal brain development in vertebrates. Disruption of TH action, either due to genetic and/or environmental factors, has been implicated in neurological defects such as autism, attention deficit hyperactivity disorders (ADHD) and IQ loss. On average, over 30 anthropogenic …
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Impact des microvésicules de plaquettes sur l'expression de produits inflammatoires des monocytes et macrophages THP-1
… surface de plusieurs types cellulaires, dont certaines peuvent les internaliser. Dans la cellule hôte, leur cargo de microARN, de facteurs de transcription, d’organites, de lipides bioactifs et d’enzymes peuvent modifier le phénotype de la cellule. Les monocytes et les macrophages, responsables …
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Notch Signaling Is Important in the Survival, Proliferation, and Self-Renewal of the Putative Breast Cancer Stem Cell Population
… reverse transcriptase-polymerase chain reaction (RT-qPCR) and confirmed by Western blotting. The effect of Notch inhibition on secondary mammosphere formation and colony formation in soft agar was evaluated. Furthermore, apoptosis was confirmed after treatment with one GSI, MRK003, by TUNEL assay …
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Nicotine Alters Gene Regulatory Networks in Dopaminergic Neurons in the Ventral Tegmental Area
… exposed to nicotine and used single-cell RT-qPCR to investigate expression changes. Next, we conducted a large-scale study using microarrays in order to identify mRNA and miRNA expression differences in DA neurons. We used these results to create miRNA-gene prediction networks and to …
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NLRP2 expressziójának vizsgálata különböző immortalizált sejtvonalakon
… expressziójára gyakorolt hatását különböző immortalizált humán sejtvonalakon. A kísérletek során THP-1, K562, Caco2, A549, NB4, U937, Hela és MCF-7 sejtvonalakat használtunk, melyeket 6, valamint 24 órás GM-CSF és dexametazon kezeléseknek vetettük alá. A kezelt sejtekből RNS-t izoláltunk, majd …
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The development of novel metabolic therapies for the treatment of glioblastoma
… only 15 months. With significant side effects further associated with the current standard of care, there is a desperate need for effective new therapies capable of selectively targeting this tumour. One potential solution is to exploit abnormal metabolic process unique to glioblastoma cells, …
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Coping with stress: How Reactive Oxygen and Nitrogen Species alter the transcriptional landscape of the Lyme disease spirochete Borrelia burgdorferi
… and reverse transcriptase-quantitative PCR (RT-qPCR). We probed for translational changes in targeted proteins via western blotting. Genes regulated by RpoS (including those associated with mammalian infection such as ospC, dbpA, and bba66) were downregulated in response to sublethal …
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The Phenotypic Effects and Transcript Response of Salt Stress, the Impact of Viral Infection on Salt Stress Symptoms, and the Effect of Salt Stress on Soybean Virus Vector Activity in Soybean Varieties That Vary in Chloride Uptake
… mechanisms for salt tolerance in plants is important to reduce yield loss due to salt stress. Soybean, <italics>Glycine max </italics> (L.) Merr., genotypes differ in chloride uptake with genotypes that take up chloride into foliar tissues tending to be salt-sensitive whereas those that …
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The interdependent regulation of photosynthesis and the circadian clock in wheat
… a fluorescence, leaf temperature and time-course RT-qPCR. In both cases mutation of circadian clock genes caused a loss of rhythmic oscillations of circadian outputs in continuous conditions. Under diel conditions disruption of the circadian oscillator had little effect on photosynthesis but there …
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Characterizing Human Papillomavirus Associated Oral Epithelial Dysplasia
… active infections were confirmed by performing RT-qPCR to identify E6 mRNA expression. We identified 82 cases of confirmed HPV-OED that showed a strong predilection for male patients and anatomical sites such as floor of mouth and ventral tongue. This group had a median age of 56.1 years. The …
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DIFFERENTIATION OF NEURAL STEM CELL USING SMALL MOLECULES IN 2D AND 3D CULTURE SYSTEM
… reverse transcription polymerase chain reaction (RT-qPCR) and immunocytochemistry (ICC), respectively. This study will show the effect of bioactive compounds on hNSCs differentiation in 2D and 3D culture systems.
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