Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 853 for “"RT-PCR"”.
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Detection of enteroviruses in marine waters using RT-PCR.
… focused on (i) optimization of methods for RT-PCR detection of enteroviruses in marine waters, with particular attention to the issue of sensitivity vs inhibition (ii) comparison of enterovirus detection in marine waters by cell culture and RT-PCR and (iii) development of a method for …
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Towards the development of an RT-PCR protocol for malaria parasite genes
… and characterisation of an amino acid transporter gene of the human malaria parasite, Plasmodium falciparum, as a possible therapeutic target. Since no sequence information was available, 3 '-RACE (a modified form of RT-PCR) was chosen as a method to obtain the gene sequence. Some of the …
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Development of one-step single-cell RT-PCR for the massively parallel detection of gene expression
… Highly active antiretroviral treatments (HAART) that use a combination of drugs that target the virus at different stages of its life cycle are effective at reducing the HIV plasma levels below levels detectable by the most sensitive assays. However, upon termination of HAART, HIV RNA …
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Prognostic Value of Bone Marrow Micrometastasis Detected by Nnested RT-PCR for Cytokeratin 19 and Mammaglobin in Breast Cancer
… reverse transcriptase polymerase chain reaction (RT-PCR) for the detection of keratin-19 and mammaglobin mRNA with two different pairs of primer. After operation, the patients were followed up by 3-month intervals. We studied possible correlation with tumor size, nodal involvement, stage, and …
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Development of a single real-time RT-PCR method for the group-specific identification of African horsesickness virus and bluetongue virus
… that can result from outbreaks. Bluetongue, an arthropod-transmitted disease of wild and domestic ruminants, is caused by the bluetongue virus, which is the prototype species of the genus Orbivirus. Bluetongue is also a notifiable disease because it can spread very rapidly in naive populations of …
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Detección de mamoglobina mediante Rt-Pcr como biomarcador de metástasis en nódulos linfáticos de pacientes con cáncer de seno : una revisión sistemática y metaanálisis
… de la mamoglobina detectada mediante la técnica RT-PCR, relacionada con la presencia de metástasis en nódulo centinela de pacientes con cáncer mamario. Los términos de búsqueda elegidos fueron usados para obtener publicaciones de diferentes bases de datos y el motor de búsqueda Google Scholar. La …
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Real Time RT-PCR for Direct Detection of Viable Mycobacterium Avium Subspecies paratuberculosis in Chron's Disease Patients and Association of Map Infection with Downregulation in Interferon-Gamma Receptor (INFG1) Gene in Crohn's Disease Patients
… in infected patients. The advent of real time PCR has proven to be significant in infectious disease diagnostics. In this study, real time reverse transcriptase PCR (RT-PCR) assay based on targeting mRNA of the IS900 gene unique to MAP has been developed. All variables included in RNA …
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Development and preliminary evaluation of a diagnostic assay for neurosyphilis using a reverse transscription-polymerase chain reaction approach
… reverse transcription-polymerase chain reaction (RT-PCR) approach. A critical component of this assay is the inclusion of a positive control that can be used on each sample. The size of the T. pa/lidum amplicon is 366 bp and that of the positive control is 950 bp. This positive control would allow …
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Chemosensory gene expression analysis in the argentine ant, linepithema humile
… EST project, which suggested their potential importance in Argentine ant ecology. An initial RT-PCR experiment demonstrated that OrA and OrB were expressed throughout the body. The newly available genome sequence was used to manually build additional Or gene models to test in RT-PCR. OBP and opsin …
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Real time PCR and fluorescent in situ hybridization in the detection of the physical tsate of human papillomavirus 16 and 18 in paraffin embedded cervical tissue
… sought to determine the feasibility of real time-PCR (RT-PCR) as a molecular tool in detecting the physical state, episomal versus integration of HPV 16 and 18 DNA in cervical cancers. Tyramide amplified fluorescent DNA in situ hybridization (FISH) was used to look for evidence of HPV 16/18 …
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Detection of Aneuploidy for Chromosomes 7 and 8 Using Fluorescence <i>In Situ</i> Hybridization in Patients with Aplastic Anemia and Sequencing of the Mitotic Checkpoint Gene hBUB1
… reverse transcription polymerase chain reaction (RT-PCR), followed by sequencing of the RT-PCR product. Statistical analyses were performed on FISH and sequencing results. Additional samples from patients with a variety of bone marrow disorders also underwent <em>hBUB1</em>-specific RT-PCR and …
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An evaluation of reverse transcription loop-mediated isothermal amplification (RTLAMP) for diagnosis of rabies.
… laboratory diagnostic methods. This is particularly common in underdeveloped countries. This study was aimed at evaluating Reverse Transcriptase Loop-Mediated Isothermal Amplification (RT-LAMP) as a viable alternative method to RT-PCR and DFAT for diagnosis of rabies in Zambia. RT-LAMP …
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An evaluation of reverse transcription loop-mediated isothermal amplification (RTLAMP) for diagnosis of rabies.
… laboratory diagnostic methods. This is particularly common in underdeveloped countries. This study was aimed at evaluating Reverse Transcriptase Loop-Mediated Isothermal Amplification (RT-LAMP) as a viable alternative method to RT-PCR and DFAT for diagnosis of rabies in Zambia. RT-LAMP …
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Etablierung von Real-Time-PCR-Verfahren für die Diagnostik des Cytomegalovirus bei organtransplantierten Patienten
… infektiöse Ursache für Morbidität und Mortalität nach einer Transplantation gesehen. Der pp65-Antigentest ist das etablierte Standardverfahren zur Überwachung von Transplantatempfängern. Dieser Test ist jedoch zeit- und arbeitsaufwendig. Nur 60% der im pp65-Antigentest positiven …
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An alginate hydrogel matrix for the localised delivery of a fibroblast/ keratinocyte co-culture to expedite wound healing
… reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay. Histological staining demonstrated that following degradation of the scaffolds, fibroblasts secreted ECM to facilitate dermal repair. Comparison of degradation of the scaffolds over time by measuring …
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Expression des Coxsackie-Adenovirus-Rezeptors in Ovarialkarzinomzellen und ihr Bezug zur adenoviralen Gentherapie
… CAR ist der pimäre zelluläre Bindungpartner für Adenoviren und daher für die adenovirale Krebsgentherapie bedeutsam. Die CAR-Expression wurde an 24 Ovarialkarzinom-Zelllinien mit mit dem monoklonalen Anti-CAR-Antikörper RmcB in der Immunzytochemie, der konfokalen Immunfluoreszenz und …
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Detection of campyloabacter jejuni and campylobacter coli in high risk foods and environmental waters using the polymerase chain reaction
Nucleic acid amplification methods (PCR) were investigated for the detection of Campylobacter species in foods and environmental waters. A novel species-specific PCR was adapted into a high-throughput, colorimetric end-stage detection format (PCR ELISA). The sensitivity of detection of the PCR …
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Expression of Epidermal Growth Factor Receptor in hypopharyngeal cancer and Effect of EGF in Progression of hypopharyngeal cancer
… from three patients were used. We performed RT-PCR and Western blot analyses of FaDu cells. Proliferative of the FaDu cells was assayed by counting the number of the cells after treatment by EGF of different concentration of 0, 10, 30 ng/㎖. Dispersion of the cells was observed by measuring …
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Chemoreceptors From the Hawk Moth Manduca Sexta L
… matches in Genbank databases and the results of RT-PCR and quantitative real-time PCR experiments show MsOr1 is expressed only in male antennae suggesting a male-specific function for this receptor. In situ hybridization revealed MsOr1 was expressed exclusively in a single cell associated with …
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Etablierung einer Zellkultur von PDL-Fibroblasten aus parodontal erkranktem Zahnhalteapparat des Menschen
Diese Dissertation beschäftigt sich mit der Etablierung einer Zellkultur aus parodontal erkrankten PDL-Zellen unter Entwicklung einer spezifischen Antibiose zur Kultivierung und einer sich daran anschließenden vergleichenden Charakterisierung der Genexpressionsmuster von gesunden und erkrankten …
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