Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 13 of 13 for “"RRM2"”.
-
Mechanism of tamoxifen resistance in breast cancer
… cells. Moreover, ribonucleotide reductase M2 (RRM2) was revealed as a key contributor to AKT-induced tamoxifen resistance. Inhibition of RRM2 by RNAi-mediated approaches significantly reversed the tamoxifen-resistant cell growth, inhibited cell motility, and activated pro-apoptotic pathways. In …
-
Ribonucleotide Reductase Subunit Switching in Hepatoblastoma Drug Response and Relapse
… of ribonucleotide reductase (RNR) subunit M2 (RRM2) in high-risk HB. RNR is the sole enzymatic complex in mammal cells that converts ribonucleotides to deoxyribonucleotides and plays a critical role in regulating cell division and DNA repair. We found standard chemotherapy agents as well as two …
-
Investigation of the Polyprimidine Tract-Binding Protein-Associated Splicing Factor (PSF) Domains Required for the Hepatitis Delta Virus (HDV) Replication
… domain, and the RNA recognition motifs RRM1 and RRM2. I propose that the RRM1 and RRM2 domains might interact with HDV RNA, while the N-terminal domain might interact with the CTD of RNAPII for HDV RNA accumulation. Together, the above experiments provide a better understanding of how an RNA …
-
Analysis of the role of poly(A) -binding protein (PAB1) in the mRNA degradation process in yeast
… PAB1 contacts to the poly (A) tail made by the RRM2 domain are critical to stabilizing the CCR4-NOT complex and promoting deadenylation. The C-terminal globular domain of PAB1 through its contacts to eRF3 is also required for CCR4 deadenylation. In contrast, the RRM3 domain of PAB1 inhibits …
-
Protein LC-MS Using Slip Flow Chromatography
… new pathway of ubiquitination; 4) separation of RRM2 monomer and aggregates. Above results demonstrated the effectiveness of slip flow capillary for the separation of both protein and peptide separation for proteomic studies.</p>
-
Exploring Protein -Rna Interactions With Site-Directed Mutagenesis and Phage Display
… binds to the GRE sequence cooperatively with RRM2 of CUG-BP1. RRM1 of CUG-BP1 is not required for GRE recognition, but may be important for maintaining the stability of the full-length CUG-BP1."
-
Exploring Protein-Rna Interactions With Site-Directed Mutagenesis and Phage Display
… binds to the GRE sequence cooperatively with RRM2 of CUG-BP1. RRM1 of CUG-BP1 is not required for GRE recognition, but may be important for maintaining the stability of the full-length CUG-BP1.
-
Exploring protein-RNA interactions with site-directed mutagenesis and phage display
… binds to the GRE sequence cooperatively with RRM2 of CUG-BP1. RRM1 of CUG-BP1 is not required for GRE recognition, but may be important for maintaining the stability of the full-length CUG-BP1.
-
Characterization of Small Molecules Inhibiting the RNA Binding Protein HuR
… of HuR and DHTS interaction using RRM1&RRM2 tandem domains. EMSA and AlphaScreen experiments, with truncated ΔRRM1 and mutants revealed that DHTS is a competitive binder of HuR with respect of target RNA. To ameliorate the solubility of DHTS, we synthesized a number of DHTS analogs, of …
-
Characterization of the Dimerization and Salt Dependent Aggregation of the ALS-Linked Protein TDP-43
… between two RNA-recognition motifs 2 (RRM2). When both interfaces are disrupted, we isolate a tentatively assigned monomeric species. The second aim of our study was to determine the propensity for aggregation of the same family of TDP-43 mutants. Using a denaturing purification method …
-
Unique Features of PTB RRMs: Insight into Protein Motions and RNA Binding
… of the motions to protein function. PTB RRM2 and RRM3 are structurally unique in that they both have a C-terminal extension that adds a fifth -strand to the canonical four stranded -sheet, connected to -strand four by a flexible linker. This extension both extends and occludes the …
-
HDAC inhibition results in suppression of FOXM1 and genes associated with poor prognosis prostate cancer through p53 dependent and independent mechanisms
… expression of genes such as MYBL2, UHRF1, RRM2 and BRCA1 that are central in cell cycle and DNA repair, through suppression of FOXM1, a nodal regulator of these processes.<br/><br/>These results uncover a multi-modal mechanism of action for Entinostat, whereby induction of p21, independent …
-
Apobec-1 Complementation Factor (ACF) Binds and Regulates Multiple RNAs
… to determine that Cox-2 RNA interacts with ACF RRM2, suggesting that RRMs 1 and 2 together bind at least some RNA targets. UV-crosslinking assays identified discrete ACF binding sites within both the IL-6 3'UTR and the Cox-2 3'UTR. On both RNAs, these sites are consistent with regions that …