Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"RPPA"”.
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Identifying and Targeting Adaptive Changes to Bet Inhibition In Ovarian Cancer
… protein expression, reverse phase protein array (RPPA) analysis and <em>in vivo</em> (orthotopic mouse model)) experiments. The poly (ADP-ribose) polymerase (PARP) inhibitor olaparib, as well as the mechanistic target of rapamycin (mTOR) inhibitors rapamycin and INK-128 were also used. Statistical …
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Prkca: Identification of A Novel Downstream Target of Wt1
… by reverse phase protein assay analysis (RPPA), show significant correlation between <em>WT1</em> mutation status and low PRKCα expression (p= 0.00013); additionally, p-PRKCα (S657) also shows decreased expression in these samples (p= 0.00373). These data suggest that the WT1 transcription …
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Yap1 Expression Predicts Sensitivity to Dual Akt/P70S6K Inhibition In Ovarian and Uterine Malignancies
… transfection, and reverse phase protein array [RPPA]) were carried out to characterize underlying mechanisms and potential biomarkers of response.</p> <p><strong>Results: </strong>MSC2363318A decreased tumor growth and metastases in multiple murine orthotopic models of ovarian (SKOV3ip1, HeyA8, …
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Inhibition of the endosomal recycling pathway to overcome resistance to cancer therapies
… obtained from a reverse-phase protein array (RPPA) study performed by our lab that found that the endosomal recycling inhibitor primaquine downregulates ER-α and AR expression. We used Western blot, quantitative RT-PCR, and immunofluorescence microscopy to confirm the RPPA results. We found …
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Investigating The Role of Cd109 In Pancreatic Ductal Adenocarcinoma
… CD109 expression and YAP/TAZ signaling through RPPA and RNA Sequencing data. This data establishes CD109 as a cell surface protein exclusively expressed in PDAC rather than healthy pancreatic tissue, demonstrating pro-oncogenic behavior and tumor initiation potential <em>in vitro</em> and <em>in …
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Harnessing Cancer Omics to Inform Precision Oncology
… tumor samples using reverse phase protein array (RPPA) and formulated an MTOR score that improves prognosis prediction of recurrent risk given Leibovich risk scoring. Finally, we selected high quality user queries to finetune the LLM modules of DrBioRight, evaluated its responses and improved its …
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SERS & efficacy assessment of anti-cancer drug-nanoparticle conjugates
… in the form of reverse phase protein array (RPPA) analysis was carried out to assess the downstream effects of the conjugates compared with unconjugated (free) EL on drug-sensitive cells. Hierarchical cluster analysis (HCA) and interactive network maps of RPPA for downstream signal …
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Downregulation of Pax2 Suppresses Ovarian Cancer Cell Growth
… gene <em>G0S2</em>. Reverse phase protein array (RPPA) data showed that PAX2 knockdown affected several genes that are involved in apoptosis, which supports the fact that downregulation of PAX2 in PAX2-expressing ovarian cancer cells inhibits cell growth. We hypothesize that this growth inhibition …
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Molecular Determinants of Residual Disease In Ovarian Cancer
… miR-409-3p and FABP4 in ovarian cancer. Using RPPA and DESI-MS imaging techniques, we explore the downstream pathways of FABP4 and discovered that FABP4 regulates several pathways associated with metastasis as well as it affects several metabolites in ovarian cancer cells. Collectively, our …
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Modulation of Pi3K-Akt Pathway In Colorectal Cancer
… pathways using reverse phase protein array (RPPA) on (1) paired tumor biopsies from a clinical trial of MK2206 in PI3K-altered metastatic CRC, (2) PTEN<sup>loss</sup>CRC patient derived xenografts (PDX) treated with MK2206 or carrier, and (3) MK2206-treated cell lines. PD inhibition was deep …
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Relevance of Fgfr1 and Its Isoforms In Prostate Cancer Bone Metastases
… = 0.0007). Importantly, we demonstrate by RPPA analysis that FGFR1 induces expression of the anchoring filament protein ladinin 1 (LAD1) in PC3 cells. Furthermore, <em>LAD1</em> gene amplification and LAD1 expression were significantly enriched in castration-resistant human PCa bone …
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Mathematical Modeling Reveals That G2/M Checkpoint Override
… study combining reverse phase protein array (RPPA) data, molecular biology and mathematical modeling to systematically identify molecules required for DNA damage checkpoint recovery. The mTOR complex 1 (mTORC1) plays an essential role to regulate mitotic entry after irradiation. Inhibition of …
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Mathematical Modeling Reveals That G2/M Checkpoint Override Creates A Therapeutic Vulnerability In Tsc2-Null Tumors
… study combining reverse phase protein array (RPPA) data, molecular biology and mathematical modeling to systematically identify molecules required for DNA damage checkpoint recovery. The mTOR complex 1 (mTORC1) plays an essential role to regulate mitotic entry after irradiation. Inhibition of …
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Mathematical Modeling Reveals That G2/M Checkpoint Override Creates A Therapeutic Vulnerability In Tsc2-Null Tumors
… study combining reverse phase protein array (RPPA) data, molecular biology and mathematical modeling to systematically identify molecules required for DNA damage checkpoint recovery. The mTOR complex 1 (mTORC1) plays an essential role to regulate mitotic entry after irradiation. Inhibition of …
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Development of Pharmaceutical Inhibitors Targeting nNOS for Melanoma Treatment
… including melanoma. Of note, our preliminary RPPA study showed that one of the significantly upregulated genes induced by IFN-γ was cyclooxygenase-2 (COX-2). COX-2 catalyzes the first rate-limiting step in the conversion of arachidonic acid to prostaglandins, including PGE2 which leads to …
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Bst2 Isoforms: Distribution and Potential Use In Cancer Therapeutics
… evaluated through reverse phase protein array (RPPA) and revealed the statistically significant upregulation of four genes: MAPK1/MAPK3, HES1, FN1 and STAT3 (p value < 0.05). All these genes play different roles in the survival mechanisms that breast cancer cells may initiate when a critical …
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Altered Ovarian Cancer Metabolism Increases Neuronal N-Acetylaspartate to Promote Tumor Growth
… profiles. Reverse Phase Protein Array (RPPA) and gene expression array were evaluated in HeyA8 ovarian cancer cell lines to investigate the protein and gene expression changes associated with NAT8L siRNA. In vitro and in vivo v experiments of NAT8L siRNA were investigated to evaluate its …
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The Role of Gdf15 In Ovarian Cancer
… GDF15 affects tumor composition. In addition, RPPA and RNA-seq was conducted on the mouse tumors to identify downstream targets of GDF15.</p> <p>In summary, this study showed that induction of GDF15 by platinum agents is p53 dependent. This study further showed the effect GDF15 has in ovarian …
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Combined Inhibition of Ddr1 and Cdk4/6 Induces Synergistic Effects In Er-Positive, Her2-Negative Breast Cancer With Pik3Ca/Akt1 Mutations
… Similarly, reverse phase protein array (RPPA) analysis of the xenografts revealed a greater PLK1 reduction in a combined 7rh and palbociclib treatment group. Our data indicate that DDR1 inhibition can augment the cell cycle suppressive effect of palbociclib and could be an effective …
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Circumventing Cisplatin Resistance In Ovarian Cancers Through Reactivation of P53 By Non-Cross-Resistant Platinum Analogs
… at Ser20 through a Chk2 independent pathway. RPPA analysis has identified the MAPK pathway as a possible target of activation by oxali-Pt to phosphorylate p53 at Ser20. Systematic studies using targeted inhibitors have identified MEK1/2, but not ERK1/2, as a novel biomarker important to …
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