Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 119 for “"RP-HPLC"”.
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Determination of platinum, palladium and rhodium, in aqueous media by means of reversed phase-high performance liquid chromatography (RP-HPLC) after complexation with N,N-dialkyl-N'-acylthioureas
… Phase-High Performance Liquid Chromatography (RP-HPLC) and a UV detector. A simple method of complexation of platinum(II) and palladium(II) in acidic, aqueous samples was developed. This method involves the mixing of excess ligand (HL") in acetonitrile with aqueous solutions containing traces …
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Identification and Characterization of a New Class of Bilin Lyases in Synechococcus sp. PCC 7002
… (PC) in vitro. Tryptic digestion and C18 RP-HPLC confirmed that CpcSU attached bilin at â-82 cysteine. CpcT was also shown to be a lyase specific for â-153 cysteine as confirmed by tryptic digestion and C18 RP-HPLC. CpcSU together and CpcT alone act as phycobiliprotein lyases that added …
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The isolation, partial purification and preliminary characterization of a growth factor from chick embryo brains
… In addition, CBGF stimulates 3H-thymidine incorporation in chick embryo brain astrocytes in vitro. In this study, cultured chick embryo brain non-neuronal cells were employed in a bioassay to monitor CBGF activity throughout various stages of its pllrification. Cell culture and assay conditions …
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Entwicklung von bioinformatischen Visualisierungswerkzeugen für Metabolitdaten von Nährstoffmangelsituationen bei Arabidopsis thaliana
… anhand bioinformatischer Methoden und Interpretation eines vorhandenen Messdatensatz (Element [ICP-MS]-, Ionen [IC]- und Metabolitdaten [RP-HPLC und GC/TOF-MS]) der Pflanze Arabidopsis thaliana getrennt in Blätter und Wurzeln. Die Pflanzen wurden den sechs Mangelsituationen der Nährstoffe …
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Development of an LC-MS/MS method for the analysis of triacylglycerols from meat and application in the discrimination of cooked meat products
… phase high performance liquid chromatography (RP HPLC) separation of animal fat triacylglycerols (TAGs) has been developed for coupling with atmospheric pressure chemical ionization tandem mass spectrometry using an ion trap mass spectrometer. The method developed offers significant …
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Development of a synthetic enzyme for detection and quantitation of Thiols
… yielded unique species based on RP-HPLC, and UV-Visible spectroscopy. After isolation by RP-HPLC, synzymes were examined for thiol oxidation under pseudo-first order conditions, and thiol oxidation rates were determined. Only the secondary ferrocene-$\beta$-cyclodextrin product …
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Separation of gliadin peptides for investigation of the injurious agent(s) in gluten sensitive enteropathy
… phase high performance liquid chromatography (RP-HPLC) method was developed to separate unhydrolyzed and hydrolyzed gliadins. The unhydrolyzed crude gliadins were resolved into 12-14 peaks, with at least five peaks that appeared to be common to all three wheat varieties. Gliadin peptides were …
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Identification and functional study of a novel antimicrobial peptide, QUB-2014, from the skin secretion of the frog, Phyllomedusa tomopterna
… the crude peptide was purified via reverse-phase HPLC (RP-HPLC). MALDI-TOF mass spectrometry was used further to confirm the target peptide from the RP-HPLC fractions. Significantly, antimicrobial assays showed that this novel peptide QUB-2014 had a broad-spectrum antimicrobial activity against …
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Analyse und Charakterisierung von Mycosporinen aus gesteinsbesiedelnden mikrokolonialen Pilzen und Umweltgesteinsproben
… herausgelöst und mit Hilfe der Kopplung von RP-HPLC und Massenspektrometrie getrennt werden. 8 Mycosporine und 5 MAAs aus verschiedenen terrestrischen und marinen Organismen wurden anhand ihrer Retentionszeiten, Molekularmassen, Fragmentierungsmuster und ihres UV-Absorptionsmaximums …
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Investigation into reliability and performance of an implantable closed-loop insulin delivery device
… formation over a period of 31 days using RP-HPLC have shown that stainless steel and titanium are the most compatible materials. Polycarbonate contributes to insulin loss after 11 days, resin might not be the best material and polyurethane is the least compatible for future device designs. …
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Isolierung und Identifizierung von Sexualpheromonen des marinen Polychaeten Platynereis dumerilii (Annelida, Polychaeta)
… die Identifizierung des Egg Release Pheromons (ERP) und des Mate Recognition Pheromons (MRP) beim marinen Polychaeten Platynereis dumerilii. Die Isolierung des ERP wurde durch biotestorientierte Fraktionierung männlicher Coelomflüssigkeit mit RP-HPLC erreicht. Das verhaltensphysiologisch inaktive …
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Untersuchungen zur Proteolyse von para-k-Casein: vom Modell zum Käse
… Methoden (SDS-Elektrophorese, IEF, GPC, RP-HPLC, ESI-MS u.a.) konnte der Abbau des para-k-Caseins durch die industriell relevanten Milchgerinnungsenzyme Chymosin, Fromase und Suparen bei unterschiedlichem Wasserangebot verfolgt werden. Para-k-Casein wird bei einem käseüblichen …
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A bioactive peptide QUB-1370 from the frog skin secretion of Pelophylax nigromaculata
… peptide and the peptide was purified by using RP-HPLC. To test the bioactivities of QUB-1370, a series assays such as antimicrobial assay, anticancer assay and haemolysis examinations were performed. However, the results showed that QUB-1370 didn't show any biological functions in these …
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A bioactive peptide from the skin of the Chinese Torrent Frog, <i>Amolops wuyiensis</i>
… its encoded mature peptide, sequenced as AVPLIYNRPGVYVTKRPK-NH2, was synthesised by Fmoc-solid phase peptide synthesis. The structure of the synthetic peptide was confirmed by matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry and purified by reversed-phase …
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Discovery and functional study of a bioactive peptide QUB-1892 from the defensive skin secretion of The Edible Frog (Pelophylax eculentus)
… by solid-phase peptide synthesis (SPPS). RP-HPLC and MALDI-TOF MS were used to purify and analyse the mature peptide. Its minimal inhibitory concentration (MIC) was obtained by using different micro-organisms and its haemolytic activity was tested by the use of horse red blood cells. The …
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Bioactive evaluations of novel peptide QUB-2552 from the skin secretion of the Chinese Large Odorous frog, Rana (Odorrana) livida
… and purified by Matrix-assisted, laser desorption, ionisation, time-offlight (MALDI-TOF) and Reversed-Phase High Performance Liquid Chromatography (RP-HPLC). QUB-2552 was subjected to functional tests and it was found to have moderate antimicrobial effect against C. albicans and S. aureus. …
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A bioactive peptide from the skin secretion of the Chinese Torrent Frog, Amolops wuyiensis
… peptides, have enormous potential for medical purposes. <br/>Here, a Kunitz-type peptide was identified from the Chinese Torrent Frog, Amolops wuyiensis by molecular cloning. Then the mature peptide was successfully synthesised by Tribute® 2-channel peptide synthesiser. Reversed-phase high …
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Identification, isolation and bioactivity assessment of a bioactive peptide QUB-1465 from the skin secretion of Hylarana guentheri
… model”, the “toroidal-pore model” and the “carpet-like model”. In this research, a single cDNA sequence encoding a bioactive peptide QUB-1465 was cloned from a cDNA library constructed from the lyophilised secretion of Hylarana guentheri. A replijavascript:void(0);cate of peptide was produced …
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Isolation and identification of an antimicrobial peptide with high anticancer activity and selectivity, QUB-2584 from the skin secretion of Litoria caerulea
… by Solid Phase Peptide Synthesis, purified by RP-HPLC, and then analysed by MALDI-TOF MS to identify the peptide. Finally, QUB-2584 was tested by biological assays, it has shown significant antimicrobial activities against S. aureus, E. coli, and C. albicans with MIC at 2, 8, and 32 μM, …
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