Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"RNases"”.
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An Investigation of Bacterial Ribonucleases as an Antibiotic Target
… is processed by bacterial ribonucleases (RNases). RNases are also involved in the degradation and turnover of this RNA during times of stress, such as the presence of an antibiotic. This makes ribonucleases a potential target for novel antibiotics.</p><p>It was shown that <em>Escherichia …
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Endoribonukleazių poveikio ankstyviesiems bakteriofago T4 transkriptams tyrimas /
… sites of RegB-processed transcripts involve RNases G and E. The RNase G appears to be the main ribonuclease that cleaves all known secondary targets. Moreover, the revealed targets are the first RNase G targets identified in the bacteriophage T4 mRNA. This study has revealed that RNase G can …
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Discovery and Characterization of a Novel Regulatory Small RNA, VcrS, Required for Virulence in Brucella abortus
… through sRNA binding to protect the mRNA from RNases. sRNA binding can also alleviate the secondary structure and reveal the ribosomal binding site. Alternatively, sRNA-mRNA interactions can have negative consequences on gene expression through degradation via RNases or sRNA binding can occlude …
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Pancreatic Ribonuclease Functions As An Epidermal Growth Factor Receptor Ligand Independently of Its Enzyme Activity and Contributes to Cetuximab Resistance
… This ligand-like activity is independent of RNases’ enzymatic activity. In addition, Gln93 and Tyr94 of hRNase5, which are highly conserved between hRNase5 and EGF, are critical for efficient binding to EGFR. A statistically positive correlation between hRNase5 and activated EGFR expression …
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CPISPR-CAS: From a Prokaryotic Immune System to a Gene Editing Tool
… genetic inactivation of the type III-A RNases Csm3 and Csm6 led to the accumulation of the target phage mRNA and abrogated immunity. Csm6 was also required to provide defense in the presence of mutated phage targets, when DNA cleavage efficiency was reduced. My results showed that the …
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Sustained hydrogel-based delivery of RNA interference nanocomplexes for gene knockdown
… delivery the RNAi molecules are degraded by RNases and flushed by excretory organs causing an inefficient duration of gene silencing effect at target tissues. These challenges can potentially be minimised by delivering RNAi molecules via non-viral nanoparticle carriers encapsulated in …
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Investigation into synthetic protease-sensitive hydrogels as siRNA nanoparticle delivery vehicles.
… and higher were able to protect the siRNA from RNases present in serum for 7 days with up to 65% of RNA still intact. A 40:1 ratio was found to be cytotoxic. A 20:1 ratio was found to be the most effective at gene knock down and determined to be optimal. As there is a relatively limited volume …
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Studio molecolare dei meccanismi dell'autoincompatibilità gametofitica in pero europeo (Pyrus communis)
… to act mediating the ubiquitination of the S-RNases, targeting them for the degradation in compatible pollen tubes. Attempts to clone SLF/SFB genes in the Pyrinae produced no results until very recently; in apple, the use of genomic libraries allowed the detection of two F-box genes linked to …
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The Biology and Biochemistry of PhoH2 proteins
… and all characterised PIN domain proteins are RNases. Most efforts have focussed on determining the role of PIN domain proteins that comprise the toxic component (VapC) of VapBC toxin antitoxin systems, in which the PIN domain is coexpressed as part of an operon with an inhibitor (VapB). In the …
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Unifying the features that determine substrate preferences of nanoRNases
… share overlapping substrates and functions, nanoRNases are unique in that they alone degrade short RNA fragments in a process that is essential for cellular fitness. Oligoribonuclease (Orn) was the first nanoRNase characterized and described for its essentiality in many bacteria, however, the …
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Time to Diagnosis and Persistence: The Two Major Determinants of Effective Tuberculosis Control
… possibly suggesting that all 47 VapCs are RNases and are targeting the same sequence. Alternatively; VapCs may belong to sub-groups targeting different sequences, allowing M. tuberculosis to exude both gross and fine metabolic control; or, they may share the same target, but are regulated …
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Characterization of A transcriptional Attenuator in The rpmf-Plsx-Fab Operon of Escherichia coli K-12
… produced by the operon are cleaved by RNases while others terminate at one of three specific points at the 5' end of plsX. This work demonstrates that a weak transcriptional terminator (an attenuator) lies at the 5' end of plsX. The attenuator was localized to a 200 bp segment. Analysis …
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The Roles of Rnt1 and Putative Endoribonucleases in Eukaryotic mRNA Degradation
… suggesting that these enzymes are broad-spectrum RNases or have an indirect role in modulating global mRNA turnover. Overall, my work uncovers a wider target range for the yeast RNase III enzyme, exposes a vital role for the well-known endonuclease in regulating protein-coding genes, identifies …
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Studies of Plant Viroid RNA and Other RNA Species of Unusual Function
… manner, and does not change the specificity of RNases T1, T2, and U2, nor does it affect the specificity of digestion by pancreatic RNase or spleen phosphodiesterase. Some difficulties arising in the handling of iodinated RNA are also discussed. In Chapter II we have employed these techniques to …