Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"RNase III"”.
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Regulation of escherichia coli ribonuclease III by the bacteriophage T7 protein kinase
Ribonuclease III (RNase III) is a double-stranded-RNA-specific nuclease of Escherichia coli. RNase III functions as a homodimer. The 25.6 kDa subunit contains two domains, a 148 residue catalytic domain and a 78 residue double-stranded-RNA-binding domain (dsRBD). RNase III is involved in the …
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Endonuclease processing in the rif region of the Escherichia coli chromosome
… it is thought that endonuclease processing by RNase E and RNase III plays some role in translational regulation and/or degradation of these transcripts. The purpose of this study was to investigate the effect that RNase E and RNase III cleavage had on the stability of transcripts derived from …
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Characterization of Inducible SMC-specific Drosha Knockout Mice
… study SMC-specific knockout of Dicer, one of two RNase III proteins in miRNA biogenesis, showed that contractile motility in the mutant intestine was dramatically decreased (Park, Yan, et al., 2011). The purpose of this study was to investigate the function of Drosha, another of the two RNase III …
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Novel Ribosome Biogenesis in the Lyme Disease Spirochete Borrelia burgdorferi
… we demonstrate the first characterization of an RNase III enzyme from a spirochete and its role in processing rRNA transcripts from the unusual rRNA gene operons of Borrelia burgdorferi. In most bacteria, the three rRNA transcripts (16S, 23S, and 5S rRNAs) that form the ribosome are produced as a …
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Novel Ribosome Biogenesis in the Lyme Disease Spirochete Borrelia burgdorferi
… we demonstrate the first characterization of an RNase III enzyme from a spirochete and its role in processing rRNA transcripts from the unusual rRNA gene operons of Borrelia burgdorferi. In most bacteria, the three rRNA transcripts (16S, 23S, and 5S rRNAs) that form the ribosome are produced as a …
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A survey of fly and nematode small RNAs by deep sequencing
… in primary transcripts through two sequential RNase III-mediated cleavages; Drosha cleaves near the base of the stem to liberate a pre-miRNA hairpin, then Dicer cleaves near the loop to generate a miRNA:miRNA* duplex. Large-scale sequencing of cDNAs derived from endogenously expressed small …
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An Investigation of Bacterial Ribonucleases as an Antibiotic Target
… is processed by bacterial ribonucleases (RNases). RNases are also involved in the degradation and turnover of this RNA during times of stress, such as the presence of an antibiotic. This makes ribonucleases a potential target for novel antibiotics.</p><p>It was shown that <em>Escherichia …
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Antibiotics that Inhibit 30S or 50S Ribosomal Subunit Formation: Hygromycin B, Quinupristin-Dalfopristin and XRP 2868.
… fragments in cells treated with hygromycin B. RNase II and RNase III deficient strains of <em>E. coli</em> exhibited the most accumulation of 16S rRNA fragments upon treatment with hygromycin B. Examination of total RNA from treated cells showed an increase in RNA corresponding to precursor to …
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Sequence determinants of pri-miRNA processing
… Microprocessor, a complex containing Drosha, an RNase III enzyme, and an RNA-binding partner DGCR8. However, it is unclear how the Microprocessor distinguishes between these hairpins and authentic pri-miRNAs. In fact, C. elegans pri-miRNAs are not processed in human cells, illustrating the …
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Regulatory Mechanism of the RNAi Pathway
… gene silencing initiated by Dicer, a RNase III that processes double-stranded RNA (dsRNA) precursors into small interfering RNA (siRNA). In Drosophila, Dicer2 and R2D2 coordinately recruit duplex siRNA to the effector RNA-induced silencing complex (RISC), wherein single-stranded siRNA …
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Diverse RNA processing pathways important for post-transcriptional gene regulation
… the miRNA. MicroRNAs mature through sequential RNase III cleavage of characteristic stem-loop precursors. Cleavage by Drosha defines the premiRNA hairpin, which is then cleaved by Dicer to generate a mature miRNA. This biogenesis pathway ensures high fidelity definition of miRNA 5' ends, which …
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Defining the Precision and Sequence Determinants of Protein Synthesis Rates
… expand and refine the known set of targets of RNase Y, its specificity factor YlbF, and RNase III. Through this, I capture sequence and structural features recognized by RNase Y, the primary endonuclease initiating RNA decay in B. subtilis. I additionally provide evidence for a novel RNA 5′ end …
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Characterization of the Genes Essential for Mouse Spermatogenesis
… hairpin structures by the cleavages of DROSHA, a RNase III enzyme, in the nucleus and DICER, another RNase III enzyme, in the cytoplasm. endo-siRNAs are distinguished from miRNAs in that endo-siRNAs are processed from naturally occurring long dsRNAs and the biogenesis of endo-siRNAs is …
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The Roles of Rnt1 and Putative Endoribonucleases in Eukaryotic mRNA Degradation
… suggesting that these enzymes are broad-spectrum RNases or have an indirect role in modulating global mRNA turnover. Overall, my work uncovers a wider target range for the yeast RNase III enzyme, exposes a vital role for the well-known endonuclease in regulating protein-coding genes, identifies …
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Fantastic ribonucleoprotein complexes and how to study them. Architectural principles of regulatory assemblies in bacteria.
… protect sRNAs from other ribonucleases such as RNase E and RNase III, and potentially lend their KH-S1 portal as an interaction platform to promote sRNA/RNA target pairing. A fraction of PNPase in the cell associates with the endoribonuclease RNase E to form the E. coli ‘RNA degradosome’, a …
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The Epstein-Barr Virus-Encoded BHRF1 miRNAs Regulate Viral Gene Expression in Cis
… EBV infection and latency. LCLs have a latency III gene expression profile akin to the EBV-associated tumors diffuse large B cell lymphoma (DLBCL) and post-transplant lymphoproliferative disorder (PTLD). During latency III, EBV expresses the full repertoire of latency genes including two …
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Epigenetics and sex expression in trioecious papaya
… sRNAs are processed from an RNA duplex by the RNase III enzyme dicer. Since the sRNA duplex is formed by Watson-Crick base pairing, equal proportion of purine-rich and pyrimidine-rich sequences are expected in the endogenous sRNA population. However, the endogenous sRNA population in papaya was …